Immunological assessment of plant-derived avian flu H5/HA1 variants

Spitsin, Sergei; Andrianov, V. M.; Pogrebnyak, Natalia; Smirnov, Yuriy; Borisjuk, Nikolai; Portocarrero, Carla; Veguilla, Vic; Koprowski, Hilary; Golovkin, Maxim

doi:10.1016/j.vaccine.2008.12.050

Cited by 27 publications

(22 citation statements)

References 20 publications

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“…Transgenic plants were produced with all four constructs using Agrobacterium -mediated transformation (Table 1). Whereas the in planta expression of hemagglutinin (H5) has already been achieved elsewhere [24,28,29], we believe that this is the first report of in planta synthesis of NA N1.…”

Section: Resultsmentioning

confidence: 80%

“…Similarly, plant-produced hemagglutinin vaccines are effective in chickens in a challenge study [28]. Plant-produced transgenic antigens have been purified from the plant matrix using either Protein A based affinity chromatography via Fc fusions [29], Ni-column chromatography and anion exchange chromatography [27], or a combination of two phase separations, several membrane filtration steps and gel filtration [28]. Here we demonstrate the purification of both ELPylated avian flu hemagglutinin and neuraminidase (NA) synthesized in transgenic tobacco plants using a membrane-based ITC (mITC) method.…”

Section: Introductionmentioning

confidence: 99%

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Membrane-Based Inverse Transition Cycling: An Improved Means for Purifying Plant-Derived Recombinant Protein-Elastin-Like Polypeptide Fusions

Phan

Conrad

2011

IJMS

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Elastin-like peptide (ELP) was fused to two different avian flu H5N1 antigens and expressed in transgenic tobacco plants. The presence of the ELP tag enhanced the accumulation of the heterologous proteins in the tobacco leaves. An effective membrane-based Inverse Transition Cycling was developed to recover the ELPylated antigens and antibodies from plant material. The functionality of both the ELPylated neuraminidase and an ELPylated nanobody was demonstrated.

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Section: Resultsmentioning

confidence: 80%

Section: Introductionmentioning

confidence: 99%

Membrane-Based Inverse Transition Cycling: An Improved Means for Purifying Plant-Derived Recombinant Protein-Elastin-Like Polypeptide Fusions

Phan

Conrad

2011

IJMS

View full text Add to dashboard Cite

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“…AIV is also recognized as an important constraint to international trade in animals and hence has substantial economic impact [41]. Recently, attention of the scientific community has focused on the development of a plant-made vaccine against avian influenza using transient transformation of Nicotiana [10], [11], [12], [42], [43], [44], [45]. These studies used purified proteins to induce an immune response thereby avoiding possible negative health consequences due to the presence of secondary metabolites such as pyridine alkaloids.…”

Section: Discussionmentioning

confidence: 99%

An Interspecific Nicotiana Hybrid as a Useful and Cost-Effective Platform for Production of Animal Vaccines

et al. 2012

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The use of transgenic plants to produce novel products has great biotechnological potential as the relatively inexpensive inputs of light, water, and nutrients are utilised in return for potentially valuable bioactive metabolites, diagnostic proteins and vaccines. Extensive research is ongoing in this area internationally with the aim of producing plant-made vaccines of importance for both animals and humans. Vaccine purification is generally regarded as being integral to the preparation of safe and effective vaccines for use in humans. However, the use of crude plant extracts for animal immunisation may enable plant-made vaccines to become a cost-effective and efficacious approach to safely immunise large numbers of farm animals against diseases such as avian influenza. Since the technology associated with genetic transformation and large-scale propagation is very well established in Nicotiana, the genus has attributes well-suited for the production of plant-made vaccines. However the presence of potentially toxic alkaloids in Nicotiana extracts impedes their use as crude vaccine preparations. In the current study we describe a Nicotiana tabacum and N. glauca hybrid that expresses the HA glycoprotein of influenza A in its leaves but does not synthesize alkaloids. We demonstrate that injection with crude leaf extracts from these interspecific hybrid plants is a safe and effective approach for immunising mice. Moreover, this antigen-producing alkaloid-free, transgenic interspecific hybrid is vigorous, with a high capacity for vegetative shoot regeneration after harvesting. These plants are easily propagated by vegetative cuttings and have the added benefit of not producing viable pollen, thus reducing potential problems associated with bio-containment. Hence, these Nicotiana hybrids provide an advantageous production platform for partially purified, plant-made vaccines which may be particularly well suited for use in veterinary immunization programs.

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“…However, in this study, recombinant GA733-2-Fc did not show any Fc effects in the antibody response against GA733-2. Spitsin et al [17] reported no detectable fusion-related enhancement of immunogenicity or quality of the immune response for fusion of the avian flu antigen with the IgG Fc fragment.…”

Section: Immunogenicity Of Recombinant Ga733-2-fc In Micementioning

confidence: 99%

Expression and Immunogenicity of a Recombinant Chimeric Protein of Human Colorectal Cancer Antigen GA733-2 and an Fc Antibody Fragment in Stably Transformed Drosophila melanogaster S2 Cells

Seok

Kim

Yoo

et al. 2010

Appl Biochem Biotechnol

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Human colorectal cancer antigen GA733-2 fused to the immunoglobin Fc fragment (GA733-2-Fc) was expressed in stably transformed Drosophila melanogaster S2 cells, and the immunogenicity of recombinant GA733-2-Fc was investigated in mice. Recombinant GA733-2-Fc was secreted into a culture medium with a molecular mass of approximately 65 kDa. Recombinant GA733-2-Fc was purified to homogeneity using affinity fractionation with Protein A sepharose 4 Fast Flow. Recombinant GA733-2-Fc proteins elicited production of specific antibodies against recombinant GA733-2 by immunization through an intraperitoneal route. Recombinant GA733-2-Fc-induced antibodies showed a binding activity to human colorectal carcinoma HCT-116 cells. Secretory recombinant GA733-2-Fc from Drosophila S2 cell systems can be used as an effective experimental antigen for research in cancer vaccine development.

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Immunological assessment of plant-derived avian flu H5/HA1 variants

Cited by 27 publications

References 20 publications

Membrane-Based Inverse Transition Cycling: An Improved Means for Purifying Plant-Derived Recombinant Protein-Elastin-Like Polypeptide Fusions

Membrane-Based Inverse Transition Cycling: An Improved Means for Purifying Plant-Derived Recombinant Protein-Elastin-Like Polypeptide Fusions

An Interspecific Nicotiana Hybrid as a Useful and Cost-Effective Platform for Production of Animal Vaccines

Expression and Immunogenicity of a Recombinant Chimeric Protein of Human Colorectal Cancer Antigen GA733-2 and an Fc Antibody Fragment in Stably Transformed Drosophila melanogaster S2 Cells

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