Immunological behavior after mycobacterial infection in selected lines of mice with high or low antibody responses

Abstract

Resistance and susceptibility to mycobacterial infection in the Biozzi high and low lines of mice which were genetically selected for their responses to heterologous erythrocytes have been found to be related to the innate ability of nonimmune macrophages to kill or inhibit the growth of the organisms during the first two weeks after infection and to their ability to mount specific and nonspecific immune responses. High antibody-producer mice were more capable of expressing cell-mediated immune parameters than low antibody-producer mice. A direct relationship was observed between the ability of bacteria (BCG vaccine) to multiply inside the reticuloendothelial system and the development of cell-mediated immunity, as measured by the delayed local reaction at the injection site, the lymphoproliferative response in the draining nodes, the tuberculin delayed-type hypersensitivity, the acquired resistance, and the adjuvant effect after BCG inoculation. In high line mice, apart from the inability of their macrophages to inhibit the early growth of bacteria, their lymphocytes in spleen and thymus were more capable of being stimulated in vitro by varying concentrations of living BCG. The data presented in this report are compatible with the hypothesis that a group of genes segregated in each line during the selective breeding controls the innate microbicidal activity.

Design and development of a multi‐epitope vaccine for the prevention of latent tuberculosis infection

“…Upon inhalation by the host, M. tuberculosis primarily targets the alveoli, which is captured by innate immune cells via pattern recognition receptors [14, 74]. The activation of these cells then triggers the host's nonspecific immune response to eliminate the invading mycobacteria [75]. Our results demonstrate that the C624P vaccine significantly increases the population of macrophages, dendritic cells, and NK cells following three rounds of injections.…”

Immune response to Mycobacterium bovis-AN5 infection in genetically selected mice (selection IV-A)

“…Lagrange et al (19) indicated that H mice, Selection I, were more resistant to virulent M. tuberculosis than L mice and were effectively protected after BCG vaccination.…”

“…Despite the inefficiency of the destruction mechanism, the infected macrophage starts to release substances to attract T lymphocytes to the infection site, through the major histocompatibility complex (MHC), class II, at the same time that it displays immunogenic epitopes (proteins and polysaccharides) on its surface. Stimulated T lymphocytes then produce lymphokines, like interferon-γ (INF-γ), that enhance macrophage efficiency during enzymatic digestion by releasing NO, a potent antimicrobial agent (2,12,14,19,21).…”

The long‐term evolution of mycobacterial BCG and preformed immune complex BCG/anti‐BCG granulomas in rats

“…lepruemurium in macrophages assisted the immune response in mice (Closs, 1975;. Similarly, mice bred for high antibody production mount a more efficient immune response, and control better a challenge with virulent H37 Rv tubercle bacilli, than do low antibody producers (Lagrange, Hurtel and Thickstun, 1979). There was, however, another difference between the two experiments, and that was that in the preformed complex lesions the antibody was purely specific.…”