Immunological conception of African swine fever

Макаров, В. В.; Недосеков, В. В.; Sereda, A.D.; Matvienko, N. I.

doi:10.1080/21658005.2016.1182822

Cited by 10 publications

(7 citation statements)

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“…Eight ASFV serogroups (SG) were defined and thoroughly characterized; however, more SG may exist. Additional parameters such as HA density (number of erythrocytes per infected cells) and erythrocyte contact maps were used to refine ASFV isolate classification (Makarov et al, 2016;Sereda et al, 1994). The HAI based assay was used for ASFV strains selection and screening for development a potential liveattenuated vaccine candidates (Sereda et al, 1992;Sereda and Balyshev, 2011).…”

Section: Antigenic Diversity Serogroupsmentioning

confidence: 99%

Genetic and antigenic diversity of African swine fever virus

Malogolovkin

Kolbasov

2019

Virus Research

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Highlights ASFV demonstrates extreme genetic diversity and use multiple mechanisms for acquisition novel phenotype characteristics  Evolutionary rate of ASFV variable regions may approach similar values to RNA viruses  More complete ASFV sequences are needed to better understand viral genome plasticity, antigenic diversity, and evolution  Heamdsorbtion inhibition assay is a reliable tool in defining ASFV antigenic diversity and candidate vaccine efficiency ABSTRACT African swine fever virus ( ASFV) is the only known DNA arbovirus, and the ability to replicate efficiently in both insect and mammalian cells is encoded in its viral genome.Despite having a relatively low overall genomic mutation rate, ASFV demonstrates genetic diversity in certain genes and complexity in gene content in other genomic regions, indicating that ASFV may exploit multiple mechanisms for diversification and acquire new phenotype characteristics. ASFV antigenic diversity is reflected in the ability to type cross-protective viruses together into serogroups, largely based on antibody-mediated inhibition of hemadsorption. Here we review ASFV genetic signatures of ASFV type specificity, genome variability, and the hemadsorption as a means of defining virus antigenic type, and how these may be used toward defining antigenic and phenotypic diversity that is problematic for development of vaccine solutions to ASF.ASFV causes explosive transboundary epidemics of ASF. In recent years, ASF has spread dramatically and now poses a threat to swine production worldwide (Cisek et al.,

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Section: Antigenic Diversity Serogroupsmentioning

confidence: 99%

Genetic and antigenic diversity of African swine fever virus

Malogolovkin

Kolbasov

2019

Virus Research

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“…Later, it was confirmed that due to the high probability of reversion and poor immunobiological characteristics of many attenuated strains the live vaccines based on them were unsuitable for widespread use [9]. The experiments to develop inactivated or subunit vaccines against ASF using conventional methods have also failed because of their inability to induce cell-mediated immunity, which plays a crucial role in the formation of protection at ASF [10][11][12]. However, the research on the development of safe protective candidate drugs for the temporary protection of swine, ensuring their protection in enzootic areas or the planned slaughter on large pig farms, is ongoing [9,13].…”

Section: Introductionmentioning

confidence: 99%

Expression of Recombinant Genes Encoding Fragments of the Protective Important Proteins of African Swine Fever Virus in Eucaryotic Cells

Imatdinov¹,

Sereda²,

Imatdinov³

et al. 2016

S-h. biol.

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, 2015). In view to develop a DNA vaccine against ASFV seroimmunotype 3 we have constructed a set of hybrid plasmids containing fragments of ASFV genes CP204L, E183L and EP402R from attenuated strain MK-200 (pCI-neo/ASFV/p30, pCI-neo/ASFV/p54 and pCI-neo/ASFV/CD2v). To study expression of the antigenically active polypeptide products for recombinant proteins rp30, rp54 and rCD2v in the eukaryotic cells, we transfected human embryonic kidney cells HEK293T, which stably express the SV40 large T antigen, with recombinant plasmids pCI-neo/ASFV/p30, pCI-neo/ASFV/p54 and pCI-neo/ASFV/CD2v. By immunoblotting, the polypeptides of the expressed recombinant proteins were identified in the HEK293T cell lysates and characterized for their molecular weights. Regarding size, some antigenically active recombinant polypeptides were as calculated, whereas the other ones apparently resulted from post translational modification. We identified a 21.6 kDa polypeptide after pCI-neo/ASFV/p30 transfection, a major (20.9 kDa) and a minor (36.3 kDa) polypeptides after pCIneo/ASFV/p54 transfection, and, finally, major polypeptides of 39.8 kDa and 63.1 kDa, together with minor polypeptides of 28.8 kDa and 104.7 kDa when pCI-neo/ASFV/CD2v transfected. These genetic constructions will be helpful to investigate antigenic, immunogenic and protective properties of ASFV recombinant proteins rp30, rp54 and rCD2v.

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“…It was shown that cytotoxic Т-lymphocytes (CTL) are important for maintenance of virus-specific protection in the early period following ASFV infection [19,20]. Cytolysis percentage of macrophages infected by homologous isolate was higher than that for heterologous isolates, which evidenced on immunotype specificity of CTL [21].…”

mentioning

confidence: 99%

“…Therefore, immune protection against ASFV is likely due to effect of killer T-lymphocytes and ADCC against viral proteins at surface of infected monocytes (macrophages) [20,23]. Synergy of CTLs and antibodies in ADCC had been proved by in vitro tests with the use of homological cultures of leukocyte cells obtained before and on day 6 post injection of high dose of the attenuated ASFV strain.…”

mentioning

confidence: 99%

MECHANISMS OF IMMUNE RESPONSE AND PROSPECTS FOR DNA VACCINES AGAINST AFRICAN SWINE FEVER (review)

Sereda¹,

Imatdinov²,

Dubrovskaya³

et al. 2017

S-h. biol.

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The agent of African swine fever (ASF) is a large envelope virus (ASFV) belonging to family Asfarviridae and containing a double-stranded linear DNA of 170 to 190 kb in size coding for more than 150 proteins, most of which are involved in host-virus interactions (L.K. Dixon et al., 2004). Its virulent isolates cause a contagious hemorrhagic disease with 100 % mortality both among domestic pigs (Sus scrofa domesticus) and wild boars (Sus scrofa). The disease control is complicated by the lack of any specific preventive methods (R.J. Rowlands et al., 2008; D.A. Chapman et al., 2011; P. Rahimi et al., 2010). The attempts to protect pigs against ASF with experimental live and inactivated subunit vaccines developed by standard methods failed (S. Blome et al., 2014). This paper discusses immunological mechanisms to provide the specific defense base on potentially protective virus-specific proteins, and immunogenic and some protective properties of ASFV gene-based DNA constructs. Immune protection at ASF is due to cytotoxic T-lymphocytes (CTL) and antibody-dependent cellmediated cytotoxicity (ADCC) effectors against viral proteins located on infected monocyte/macrophage. There is a synergism of these effectors (A.D. Sereda, 2013). Based on i) the location, structure and functional properties of viral proteins, ii) the polypeptide specificity of blood antibodies after injecting pigs with ASFV attenuated or virulent strains, iii) the effects of pig immunization using purified proteins from infected cells or the recombinant proteins, and DNN constructs, p30, p54 and CD2v proteins are considered as potentially protective (S.D. Kollnberger et al., 2002; M.G. Barderas et al., 2001; J.G. Neilan et al., 2004). A significant disadvantage of the candidate DNA vaccine is a relatively low immune response, especially in large mammals. There were attempts of overcoming the problem using various strategies (

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Immunological conception of African swine fever

Cited by 10 publications

References 1 publication

Genetic and antigenic diversity of African swine fever virus

Genetic and antigenic diversity of African swine fever virus

Expression of Recombinant Genes Encoding Fragments of the Protective Important Proteins of African Swine Fever Virus in Eucaryotic Cells

MECHANISMS OF IMMUNE RESPONSE AND PROSPECTS FOR DNA VACCINES AGAINST AFRICAN SWINE FEVER (review)

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