Immunological considerations for heart valve replacements

Atcha, Hamza; Liu, Wendy F.

doi:10.1016/b978-0-12-814661-3.00012-5

Cited by 2 publications

(1 citation statement)

References 103 publications

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“…P(3HO) is already known for its numerous advantages in terms of biocompatibility and biodegradability. Due to its beneficial elasticity (Young's modulus 11.4-34 MPa) [4,14], endurance (friction coefficient 0.198-0.202), and low fragility (hardness 3.2-4.5 MPa) [1], it is being reviewed in terms of potential heart valve replacement, in wound-healing studies, and for joint endoprostheses [15][16][17]. Although in our previous works we ran a series of experiments describing the influence of P(3HO) on cell behavior, migration, morphology, and cytoskeleton, still there are some uncovered areas of interest on the specific substrate-cell interaction [1,4,18].…”

Section: P(3ho)mentioning

confidence: 99%

Vimentin Cytoskeleton Architecture Analysis on Polylactide and Polyhydroxyoctanoate Substrates for Cell Culturing

Feliksiak

Solarz

Guzik

et al. 2021

IJMS

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Polylactide (PLA), widely used in bioengineering and medicine, gained popularity due to its biocompatibility and biodegradability. Natural origin and eco-friendly background encourage the search of novel materials with such features, such as polyhydroxyoctanoate (P(3HO)), a polyester of bacterial origin. Physicochemical features of both P(3HO) and PLA have an impact on cellular response 32, i.e., adhesion, migration, and cell morphology, based on the signaling and changes in the architecture of the three cytoskeletal networks: microfilaments (F-actin), microtubules, and intermediate filaments (IF). To investigate the role of IF in the cellular response to the substrate, we focused on vimentin intermediate filaments (VIFs), present in mouse embryonic fibroblast cells (MEF). VIFs maintain cell integrity and protect it from external mechanical stress, and also take part in the transmission of signals from the exterior of the cell to its inner organelles, which is under constant investigation. Physiochemical properties of a substrate have an impact on cells’ morphology, and thus on cytoskeleton network signaling and assembly. In this work, we show how PLA and P(3HO) crystallinity and hydrophilicity influence VIFs, and we identify that two different types of vimentin cytoskeleton architecture: network “classic” and “nutshell-like” are expressed by MEFs in different numbers of cells depending on substrate features.

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