Immunological Cross-Reactivity Involving Mollusc Species and Mite–Mollusc and Cross-Reactive Allergen PM Are Risk Factors of Mollusc Allergy

Abstract

Marine molluscs are seafood consumed worldwide and could cause food allergies, while investigation on their sensitizing components and cross-reactivity seems to be rare. Furthermore, allergy to mites may result in anaphylaxis in molluscallergic individuals owing to their cross-reactivity. The aim of the study was to identify cross-reactive allergens and investigate the cross-reactivity between different mollusc groups and mite−mollusc. The extracted mollusc and dust mite proteins were separated by SDS-PAGE, and IgE-binding components were recognized by immunoblotting with sera from patients sensitized to mollusc and mite. Cross-reactivity of different mollusc groups and mite−mollusc was assessed using ELISA and inhibition ELISA. The results of the immune detection, ELISA, and inhibition ELISA indicated that different mollusc groups and mite−mollusc showed varying degrees of cross-reactivity. The most frequently recognized cross-reactive protein was paramyosin from different mollusc groups and dust mite, while cross-reactive allergen paramyosin in the mite extract was identified and evaluated by MS and Allermatch, respectively. Inhibition ELISA studies also revealed that paramyosin played an important role in molluscan and mite−molluscan cross-reactivity. These findings contribute to a better understanding of the cross-reactivity involving mollusc species and mite− mollusc, which can be used to assist in the diagnosis and treatment of mite-and mollusc-allergic disorders.

Emerging approaches in the diagnosis and therapy in shellfish allergy

“…Filamin C and paramyosin from are on the other hand identified from mud crab ( Scylla paramamosain ) and veined rapa whelk ( Rapana venosa ) respectively [19 ▪ ,20 ▪ ]. By means of inhibition ELISA, Yu et al [21 ▪▪ ] showed that paramyosin played an important role in molluscan and mite-molluscan cross-reactivity, and the relevance of these proteins in other edible shellfish would warrant more characterization. Karnaneedi et al [22 ▪▪ ] recently revealed up to 39 unreported allergens from five shrimp species including whiteleg shrimp ( L. vannamei ), black tiger shrimp ( P. monodon ), banana shrimp ( Fenneropenaeus merguiensis ), king shrimp ( Melicertus latisculcatus ) and endeavour shrimp ( Metapenaeus endeavouri ) by de novo transcriptome assembly and BLAST searching against known allergens on WHO/IUIS and AllergenOnline.…”