Immunological detection of <i>Kudoa thyrsites</i> spores in muscle tissues of farmed Atlantic salmon, <i>Salmo salar</i> L.

Chase, J C; Booy, M H; Dawson-Coates, J A; Haddow, Jody D.; Haines, Lee R.; Whitaker, David; Olafson, Robert W.; Pearson, Terry W.

doi:10.1046/j.1365-2761.2003.00469.x

Cited by 4 publications

(2 citation statements)

References 6 publications

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“…E. leei spores were demonstrated to be immunogenic for rabbits, as the aPabEleei specifically detected surface epitopes on spore valves by immunohistochemistry (Estensoro et al 2013). The immunogenicity of myxospores in organisms different than the corresponding hosts (mice and rabbits) has already been observed in other studies (Clouthier et al 1997, Chase et al 2001, 2003. Furthermore, the role of myxospores in resistance development in fish has been suggested.…”

Section: Discussionmentioning

confidence: 77%

Antigenic characterization of Enteromyxum leei (Myxozoa: Myxosporea)

Estensoro¹,

Álvarez-Pellitero²,

Sitjà‐Bobadilla³

2013

Dis. Aquat. Org.

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Enteromyxum leei, an intestinal myxozoan parasite affecting a wide range of fish, was partially purified, and the immunogenic composition of its glycoproteins as well as the proteolytic activity were studied. Parasite extracts, mainly containing spores, were separated by SDS-PAGE, and thereafter, immunoblots were carried out with a polyclonal antiserum (Pab) raised against E. leei. Periodic acid/Schiff staining of gels, periodate- and Proteinase K-treated Western blots and Lectin blots were performed to analyse the terminal carbohydrate composition of the parasite's antigens. Additionally, the cross-reaction of the parasite extracts with a Pab raised against the polar filament of the myxozoan Myxobolus pendula was studied. Both Pabs detected proteic epitopes on antigenic proteins and glycoproteins of E. leei, ranging between 15 and 280 kDa. In particular, 2 glycoproteic bands (15 and 165 kDa), immunoreactive to both Pabs and with glucose and mannose moieties, could correspond to common antigens shared among myxozoans. The 165 kDa band also presented galactose, N-acetyl-galactosamine and N-acetyl-glucosamine, pointing to its possible origin on chitin-built spore valves and to its possible involvement in host-parasite interactions. The molecular weight of the 15 kDa glycoproteic antigen matches that of minicollagen, a cnidarian-specific protein of nematocysts with a myxozoan homologue. Several proteases with apparent molecular weights ranging between 43 and 245 kDa were found in zymographies of E. leei extracts, and these may have a potential role in the parasite's pathogenesis. This is a useful approach for further studies to detect targets for antiparasitic therapy.

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Section: Discussionmentioning

confidence: 77%

Antigenic characterization of Enteromyxum leei (Myxozoa: Myxosporea)

Estensoro¹,

Álvarez-Pellitero²,

Sitjà‐Bobadilla³

2013

Dis. Aquat. Org.

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“…Serial sections (5 µm) were alternately assayed with either ISH or IHC, allowing direct comparison between locations on sequential slides. We tested 4 monoclonal antibodies (mAb: 2F4, 4H2, 1H2 and 3E8; Chase et al 2001) and 1 polyclonal antibody (pAb) raised in rabbits against mature Kudoa thyrsites spores as described in Chase et al (2003). Tissue sections were deparaffinized and rehydrated in phosphate-buffered saline (PBS) (pH 7.4), plus 0.15% Tween-20 (PBS/T).…”

Section: Methodsmentioning

confidence: 99%

Epitopes associated with mature spores not recognized on Kudoa thyrsites from recently infected Atlantic salmon smolts

Young¹,

Jones²

2005

Dis. Aquat. Org.

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Atlantic salmon Salmo salar skeletal muscle was examined for Kudoa thyrsites by polymerase chain reaction (PCR) and positive fish were further examined by in situ hybridization (ISH) and immunohistochemistry (IHC). The infection was detected in 42% of salmon by PCR following a 60 d exposure to infective seawater at a temperature of 10°C (= 600 degree-days, °D). The parasite was detected by ISH in skeletal and cardiac muscle but not in gill, kidney, spleen, liver, stomach, intestine, pyloric caeca and skin. None of 4 monoclonal antibodies (2F4, 4H2, 1H2, 3E8) raised against mature K. thyrsites spores reacted with the stages identified by ISH following a 600 °D exposure, but they did react with ISH-identified stages following a 1600 °D exposure. In contrast, a polyclonal antibody reacted with K. thyrsites stages in salmon with both 600 and 1600 °D exposures, suggesting that the parasite observed in 600 °D infections represents an antigenically distinct developmental stage of K. thyrsites.

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An enzyme linked immunosorbent assay for the detection of Kudoa thyrsites in Atlantic salmon Salmo salar

Taylor

Jones

2005

Aquaculture

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Immunological detection of Kudoa thyrsites spores in muscle tissues of farmed Atlantic salmon, Salmo salar L.

Cited by 4 publications

References 6 publications

Antigenic characterization of Enteromyxum leei (Myxozoa: Myxosporea)

Antigenic characterization of Enteromyxum leei (Myxozoa: Myxosporea)

Epitopes associated with mature spores not recognized on Kudoa thyrsites from recently infected Atlantic salmon smolts

An enzyme linked immunosorbent assay for the detection of Kudoa thyrsites in Atlantic salmon Salmo salar

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