Immunological detection of keratan sulfate in meat products with and without mechanically separated chicken meat

Nakano, T.; Ozimek, L.; Betti, Mirko

doi:10.1016/j.meatsci.2012.06.015

Cited by 5 publications

(5 citation statements)

References 8 publications

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“…The immunodiffusion method and the anti-KS antibody are the same of those reported in Ref. [29]. In Ref.…”

Section: Methods Of Detection and Analysismentioning

confidence: 88%

“…The study of Nakano and coworkers aimed at detecting KS products containing mechanically separated chicken meat (MSCM) with cartilage particles [29]. To accomplish the analytical work, drydefatted samples of MSCM and meat products, containing or not MSCM, were digested with papain and non-dialyzable fractions of each digestion were examined by immunodiffusion analysis using an anti-KS monoclonal antibody belonging to the IgM family.…”

Section: Methods Of Detection and Analysismentioning

confidence: 99%

“…Antibody-based detection was negative for all samples of meat products without MSCM, while the samples of MSCM and all samples of meat products containing MSCM gave clear reaction with the antibody. The immunodiffusion test described at this reference seems to be a very simple and sensitive method for qualitative analysis of KS, especially in case of detection of KS in MSCM-included meat products [29].…”

Section: Methods Of Detection and Analysismentioning

confidence: 99%

See 2 more Smart Citations

Keratan sulfate: An up-to-date review

Pomin

2015

International Journal of Biological Macromolecules

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“…The immunodiffusion method and the anti-KS antibody are the same of those reported in Ref. [29]. In Ref.…”

Section: Methods Of Detection and Analysismentioning

confidence: 88%

Section: Methods Of Detection and Analysismentioning

confidence: 99%

Section: Methods Of Detection and Analysismentioning

confidence: 99%

See 1 more Smart Citation

Keratan sulfate: An up-to-date review

Pomin

2015

International Journal of Biological Macromolecules

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“…Other trials focused on the detection of cartilage glycosaminoglycan (keratan sulphate) by immunodiffusion analysis using anti-keratan sulfate monoclonal antibody (IgM) in meat products containing mechanically separated chicken meat (MSCM) having cartilage particles (Nakano et al, 2012). The immunodiffusion test appears to be a simple sensitive specific method for qualitative analysis of keratan sulfate, but should be used in combination with other methods.…”

Section: Immunological Methodsmentioning

confidence: 99%

Scientific Opinion on the public health risks related to mechanically separated meat (MSM) derived from poultry and swine

Hald

Baggesen

2013

EFS2

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The purpose of this assessment was to identify public health risks linked to mechanically separated meat (MSM) types from pork and poultry and compare them with fresh meat, minced meat and meat preparations (non-MSM); and to select, rank and suggest objective measurement methods and values for parameters to distinguish MSM types. Microbial hazards in MSM are expected to be similar to those in non-MSM, although the risk of microbial growth increases with the degree of muscle fibre degradation, thus with the separation pressure. For the distinction between the different types of MSM and non-MSM chemical, histological, molecular, textural and rheological parameters were considered as potential indicators. The analysis of available published data suggested that calcium and, if confirmed cholesterol content, was the only appropriate chemical parameters which could be used to distinguish MSM from non-MSM products. On the basis of published data, a model was developed to derive probabilities for a product to be classified as MSM based on the calcium content. Calcium content of 100 mg/100 g, as specified in the Reg. (EC) No. 2074/2005, corresponds to probability of 93.6% for a product to be classified as MSM, according to the model developed. Calcium content alone does not allow differentiation between low pressure MSM and other meat products, and other validated tests would be necessary. Histological parameters considered include microscopic detection of different tissues and their changes. The latter is a promising method for distinction of MSM types, but further validation is needed. In order to improve methods for MSM identification, specifically designed studies for the collection of data obtained by standardised methods on indicators such as calcium and cholesterol should be undertaken, while studies based on combinations of different parameters could also be useful

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“…Finally, KS was detected in batches from shark cartilage, averaging ~16% of the total GAGs [26]. This is most important, considering that KS is immunogenic, being able to produce immunological responses in humans [27,28].…”

Section: Pharmaceutical Grade Chondroitin Sul-fate Is the Active Drugmentioning

confidence: 98%

Condrosulf<sup>®</sup>: Structural Characterization, Pharmacological Activities and Mechanism of Action

Volpi¹

2014

CMC

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Condrosulf(®) is a pharmaceutical formulation containing chondroitin sulfate (CS) as an active component possessing high quality and purity and standardized properties. CS is currently applied as a SYSADOA (Symptomatic Slow Acting Drug for Osteoarthritis) agent in Europe in the treatment of osteoarthritis (OA). Furthermore, Condrosulf(®) and pharmaceutical grade CS have also been proven to possess structure-modifying effects belonging to the S/DMOAD (structure/disease modifying anti-osteoarthritis drug) class. This review summarizes current knowledge on CS/Condrosulf(®) structure and its properties, its pharmacological activity as proved by many clinical trials and metaanalysis studies and focuses attention on its mechanisms of action in the pathophysiology of osteoarthritic joint tissues. Finally, future perspectives are discussed in connection with the possibility to apply new outcome measures, such as MRI and biomarkers, which can yield important advances in the use of Condrosulf(®) as well as the development of new drugs with different structures useful in particular for the treatment of inflammatory symptoms and able to retard the progression of arthritis.

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Immunological detection of keratan sulfate in meat products with and without mechanically separated chicken meat

Cited by 5 publications

References 8 publications

Keratan sulfate: An up-to-date review

Keratan sulfate: An up-to-date review

Scientific Opinion on the public health risks related to mechanically separated meat (MSM) derived from poultry and swine

Condrosulf<sup>®</sup>: Structural Characterization, Pharmacological Activities and Mechanism of Action

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Immunological detection of keratan sulfate in meat products with and without mechanically separated chicken meat

Cited by 5 publications

References 8 publications

Keratan sulfate: An up-to-date review

Keratan sulfate: An up-to-date review

Scientific Opinion on the public health risks related to mechanically separated meat (MSM) derived from poultry and swine

Condrosulf<sup>&#174;</sup>: Structural Characterization, Pharmacological Activities and Mechanism of Action

Contact Info

Product

Resources

About

Condrosulf<sup>®</sup>: Structural Characterization, Pharmacological Activities and Mechanism of Action