Immunological effects of feeding macroalgae and various vitamin E supplements in Norwegian white sheep-ewes and their offspring

Novoa-Garrido, Margarita; Aanensen, Lise; Lind, Vibeke; Larsen, H.J.S.; Jensen, Søren Krogh; Govasmark, Espen; Steinshamn, Håvard

doi:10.1016/j.livsci.2014.05.021

Cited by 18 publications

(9 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…*P < 0.05; **P < 0.01. effects of dietary antioxidants on immunoglobulin production in ruminants (Chew, 1996;Novoa-Garrido et al, 2014). Overall, our results suggest an immunomodulatory effect of grape seed, as evidenced by the reduction in IgG titers on d 60 and in SFT.…”

Section: Immunological Determinationssupporting

confidence: 57%

Effects of diets containing grape seed, linseed, or both on milk production traits, liver and kidney activities, and immunity of lactating dairy ewes

Nudda

Correddu

Marzano

et al. 2015

Journal of Dairy Science

View full text Add to dashboard Cite

This study aimed to evaluate the effects of the dietary inclusion of grape seed, alone or in combination with linseed, on milk production traits, immune response, and liver and kidney metabolic activity of lactating ewes. Twenty-four Sarda dairy ewes were randomly assigned to 4 dietary treatments consisting of a control diet (CON), a diet containing 300 g/d per head of grape seed (GS), a diet containing 220 g/d per head of extruded linseed (LIN), and a diet containing a mix of 300 g/d per head of grape seed and 220 g/d per head of extruded linseed (MIX). The study lasted 10 wk, with 2 wk of adaptation period and 8 wk of experimental period. Milk yield was measured and samples were collected weekly and analyzed for fat, protein, casein, lactose, pH, milk urea nitrogen, and somatic cell count. Blood samples were collected every 2 wk by jugular vein puncture and analyzed for hematological parameters, for albumin, alkaline phosphatase, bilirubin, creatinine, gamma glutamyltransferase, aspartate aminotransferase, alanine aminotransferase, protein, blood urea nitrogen, and for anti-albumin IgG, IL-6, and lymphocyte T-helper (CD4(+)) and lymphocyte T-cytotoxic (CD8(+)) cells. On d 0, 45, and 60 of the trial, lymphocyte response to phytohemagglutinin was determined in vivo on each animal by measuring skin-fold thickness (SFT) at the site of phytohemagglutinin injection. Humoral response to chicken egg albumin was stimulated by a subcutaneous injection with albumin. Dietary treatments did not affect milk yield and composition. Milk urea nitrogen and lactose were affected by diet × period. Diets did not influence hematological, kidney, and liver parameters, except for blood urea nitrogen, which decreased in LIN and increased in MIX compared with CON and GS. Dietary treatments did not alter CD4(+), CD8(+), and CD4(+)-to-CD8(+) ratio. The SFT was reduced in GS and MIX and increased in LIN compared with CON. The IgG and IL-6 were affected by diet × period. The reduction in IgG on d 60 and SFT in ewes fed GS suggests an immunomodulatory effect of this residue. The limited variation in milk and hematological and metabolic parameters suggests that GS and LIN can be included, alone or in combination, in the diet of dairy ewes without adverse effects on milk production and health status.

show abstract

Section: Immunological Determinationssupporting

confidence: 57%

Effects of diets containing grape seed, linseed, or both on milk production traits, liver and kidney activities, and immunity of lactating dairy ewes

Nudda

Correddu

Marzano

et al. 2015

Journal of Dairy Science

View full text Add to dashboard Cite

show abstract

“…Recently, there is an increased interest in studying the effects of marine algae supplementation on sheep health and production (Bichi et al 2013;Caroprese et al 2014;Novoa-Garrido et al 2014;Ciliberti et al 2016). The supplementation in the diet of goats and cows of micro-algae, such as Spirulina platensis and Chlorella kessleri, resulted in an enrichment of nutraucetical fatty acids of milk with health benefit for consumers (Póti et al 2015).The effects of marine algae supplementation depend on basal diet composition and level of algae used in the diet (Reynolds et al 2006).…”

mentioning

confidence: 99%

Polyunsaturated fatty acid supplementation: effects of seaweed Ascophyllum nodosum and flaxseed on milk production and fatty acid profile of lactating ewes during summer

Caroprese

Ciliberti

Marino

et al. 2016

Journal of Dairy Research

View full text Add to dashboard Cite

The research reported in this Research Communication was undertaken to evaluate the effects of different sources of polyunsaturated fatty acids (PUFA) supplemented in the diet on milk production and milk fatty acid profile of lactating ewes exposed to long term heat stress. The experiment was conducted during summer, involved 32 ewes divided into 4 groups of 8 each, and lasted 6 weeks. The ewes in all groups were fed twice daily and received 1·8 kg/d of oat hay and 1 kg/d of concentrate. Flaxseed group (FS) was supplemented with 250 g/d of whole flaxseed, Ascophyllum nodosum group (AG) was supplemented with 25 g/d of seaweed Ascophyllum nodosum, and the combination group (FS + AG) received both flaxseed and Ascophyllum nodosum supplementation. The control group (CON) was fed with 1 kg/d of pelleted concentrate without PUFA supplementation. Milk samples were collected twice daily per week, and analysed for fat, total protein, casein, and lactose content. At the beginning and then at 2, 4 and 6 week of the experiment each milk sample was analysed for milk fatty acids. Temperature-humidity index (THI) was calculated daily. Supplementation of flaxseed and of the combination of flaxseed and Ascophyllum nodosum increased milk yield. The total content of saturated fatty acids (SFA) in milk decreased for ewes fed FS, followed by FS + AG. On the contrary, monounsaturated fatty acids (MUFA) increased for ewes fed FS and FS + AG. The total n-3 FA was found higher in FS and FS + AG than in AG and CON groups mainly because of the increase in C 18 : 3 n-3 in FS and FS + AG milk. Milk from FS + AG resulted in the highest n-3/n-6 ratio and decreases in atherogenic and thrombogenic indices. The combination of seaweed Ascophyllum nodosum and flaxseed can be suggested as an adequate supplementation to sustain milk production and milk fatty acid profile of sheep during summer season.

show abstract

“…Several studies evaluated the possible effects of A. nodosum utilization in animal diet to improve the oxidative state and immune functions during stressful situations (Saker et al, 2004;Archer et al, 2007;Novoa-Garrido et al, 2014). However, a direct effect of A. nodosum dietary supplementation on PBMC proliferation in sheep was not investigated.…”

Section: Discussionmentioning

confidence: 99%

“…This study is the first attempt to investigate this relation, to better explain the effect of A. nodosum on immune regulation found by other research groups (Saker et al, 2004;Novoa-Garrido et al, 2014).…”

mentioning

confidence: 80%

“…Recently, A. nodosum was administrated in the diet of dairy cows and sheep to verify the effects on their hematological parameters and immunological responses. An increase of blood glucose and a decrease in sorbitol dehydrogenase in dairy cows was found (Karatzia et al, 2012;Novoa-Garrido et al, 2014). To the best of our knowledge, no studies have evaluated the effects of supplementation of A. nodosum to dairy sheep during summer, and on the activation of their inflammatory responses in terms of cytokine secretion.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Alterations in sheep peripheral blood mononuclear cell proliferation and cytokine release by polyunsaturated fatty acid supplementation in the diet under high ambient temperature

Ciliberti

Albenzio

Annicchiarico

et al. 2015

Journal of Dairy Science

View full text Add to dashboard Cite

The aim of this study was to investigate the effects of polyunsaturated fatty acid (PUFA) supplementation from different sources in the diet of dairy sheep under high ambient temperatures on ex vivo lymphocyte proliferation and inflammatory responses. The experiment was carried out during summer: 32 Comisana ewes were divided into 4 groups of 8. The FS group was supplemented with whole flaxseed, the AG group was supplemented with Ascophyllum nodosum, the FS+AG group was supplemented with a combination of flaxseed and A. nodosum. The fourth group (CON group) was a control and received a diet containing no supplement. The average maximum temperature was around 33°C during wk 2 and 3, whereas the mean temperature never decreased below 26°C. Following 15 d of treatment with respective diets, peripheral blood mononuclear cells (PBMC) from sheep who received a diet supplemented with A. nodosum had impaired cell proliferation responses and IL-6 production after mitogen stimulation compared with PBMC from FS+AG sheep. In addition, PBMC from AG sheep displayed impaired cell proliferation compared with cells from the CON group. The FS+AG cells produced lower levels of IL-10 than CON cells, and higher IL-6 than AG and CON cells. Results demonstrated that the supplementation with PUFA from different sources in a sheep's diet can influence their immunological responses under high ambient temperatures depending on the composition of fatty acid supplementation. In particular, synergistic effects of different PUFA from flaxseed and A. nodosum, simultaneously administrated in the sheep diet, were observed on activation of inflammation response.

show abstract

Immunological effects of feeding macroalgae and various vitamin E supplements in Norwegian white sheep-ewes and their offspring

Cited by 18 publications

References 41 publications

Effects of diets containing grape seed, linseed, or both on milk production traits, liver and kidney activities, and immunity of lactating dairy ewes

Effects of diets containing grape seed, linseed, or both on milk production traits, liver and kidney activities, and immunity of lactating dairy ewes

Polyunsaturated fatty acid supplementation: effects of seaweed Ascophyllum nodosum and flaxseed on milk production and fatty acid profile of lactating ewes during summer

Alterations in sheep peripheral blood mononuclear cell proliferation and cytokine release by polyunsaturated fatty acid supplementation in the diet under high ambient temperature

Contact Info

Product

Resources

About