Immunological equivalence between mouse brain-derived and Vero cell-derived Japanese encephalitis vaccines

Current use and development of vaccines for Japanese encephalitis

“…Preclinical studies with the Kaketsuken vaccine in mice showed that it was as immunogenic as inactivated mouse-brain derived vaccine and induced antibodies capable of neutralising multiple genotype I and III JEV strains [80] . Consistent with these findings, studies using monoclonal antibodies identified no significant differences in presentation of critical neutralising epitopes on the Vero cell-derived vaccine compared with the mouse-brain derived vaccine [81] . A subsequent Phase I clinical trial in 269 adult males identified a low rate of mild adverse events and greater or equivalent seroconversion rates compared to mouse-brain-derived vaccine [82] .…”

Japanese Encephalitis: Epidemiology and Current Perspectives in Vaccines

“…A phase 1 study administering the test and conventional MBDI vaccine (n=30 each) showed no SAEs, few mild AEs (6.7%) and seroconversion of 100% (95% CI, 88%-100%) after 3 doses [84]. Immunological agreement with the conventional MBDI vaccine has also been demonstrated and a promising phase 3 trial has also been recently completed [85]. Development appears to be limited to the Japanese market only [75].…”

Estimation of Lactose Interference in Vaccines and a Proposal of Methodological Adjustment of Total Protein Determination by the Lowry Method

“…In Japan, freeze-dried, cell culture-derived JE vaccines (inactivated) developed by two Japanese manufacturers are utilized for JE prevention (11)(12)(13). JE vaccines are prepared by growing the JE virus (Beijing strain) in Vero cells (derived from African green monkey kidney cells) and then inactivating the resulting virus with formalin.…”