Immunological evaluation of the intestinal mucosa of broiler chicks treated with Lactobacillus Spp. and challenged with Salmonella Enteritidis

Okamoto, Adriano Sakai; Filho, RL Andreatti; Lima, Edna Tereza de; Pereira, R. F.; Menconi, A.; Rocha, Ticiana Silva; Marietto-Gonçalves, GA

doi:10.1590/s1516-635x2007000400009

Cited by 12 publications

(8 citation statements)

References 6 publications

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“…Christensen et al, (2002) suggested that some of these effects were mediated by cytokines secreted by immune system cells stimulated with probiotic bacteria. On the other hand, Okamoto et al, (2007) demonstrated that this type of treatment had few beneficial effects for chicks, particularly during the first days of life.…”

Section: Results Listed In Tablementioning

confidence: 99%

A Trial to Prevent Salmonella Enteritidis Infection in Broiler Chickens Using Autogenous Bacterin Compared with Probiotic Preparation

El-Ghany¹,

El-Shafii²,

Hatem³

et al. 2012

JAS

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This study was carried out to investigate the efficacy of the locally prepared autogenous Salmonella Enteritidis (S. Enteritidis) bacterin as well as a probiotic preparation in the prevention of broiler chickens from S. Enteritidis infection. A total of three hundred and ten, one day-old Hubbard broiler chicks were used. At day old, ten chicks were sacrificed and examined bacteriologically to prove their freedom from S. Enteritidis infection. Three hundred birds were divided into four equal groups. Chickens in group (1) were kept as blank control negative non infected-non treated birds, while those of group (2) were challenged non treated birds. Group (3) was vaccinated intramuscularly by the autogenous bacterin at the first day of age in a dose of 0.2 ml/bird and boostered as a second dose at 10 days of age in a dose 0.5 ml/bird, however, group (4) was given a commercial probiotic preparation as 1gm/ 4 liter of the drinking water from the first day of age and continued for 5 successive days. All birds in groups 2, 3, and 4 were challenged orally by 0.5 ml containing 10 9 CFU/ml S. Enteritidis at 20 days of age. All the groups were kept under complete observation for three weeks for recording signs, moralities, gross lesions, shedding rate of S. Enteritidis, re-isolation of the organism, the performance as well as detection of the titer of antibodies serologically using microagglutination test and enzyme linked immunosorbant assay (ELISA) test. The results showed that the both the bacterin and the probiotic are equally effective in reducing signs, mortalities, gross lesions, the shedding rate and the re-isolation of S. Enteritidis and also increasing in the performance of chickens. The effect of the bacterin and the probiotic was significant (P≤0.05) when compared with the infected non treated chickens. Moreover, the serological investigation revealed an improvement in the titer of antibodies after vaccination and probiotic treatment. In conclusion, double doses of locally prepared autogenous S. Enteritidis bacterin and the probiotic preparation were effective and safe methods for prevention of S. Enteritidis infection in broiler chickens.

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Section: Results Listed In Tablementioning

confidence: 99%

A Trial to Prevent Salmonella Enteritidis Infection in Broiler Chickens Using Autogenous Bacterin Compared with Probiotic Preparation

El-Ghany¹,

El-Shafii²,

Hatem³

et al. 2012

JAS

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“…It was centrifuged at 3000 rpm for 10 min and the sediment was diluted with sterile phosphate buffer saline and adjusted to contain 10 9 CFU/mL using a McFerland matching tube to be used for challenging. According to the method of Timms [22], the challenge inoculum was prepared at 28 days of age, each bird in the challenged groups was infected orally with 0.5 mL suspension containing 10 9 CFU/mL SE [23]. The commercial balanced ration that met the broiler chicken requirements according to the National Research Council [24] was used as follows: starter (23% crude protein and metabolizable energy 3008 Kcal/kg), grower (21% crude protein and 3080 Kcal/kg diet) and finisher feed (19% crude protein and 3190 Kcal/kg diet) until 12, 26 and 42 days of age, respectively.…”

Section: The Challenge Bacteriummentioning

confidence: 99%

The Simultaneous Administration of a Probiotic or Prebiotic with Live Salmonella Vaccine Improves Growth Performance and Reduces Fecal Shedding of the Bacterium in Salmonella-Challenged Broilers

El‐Shall

Awad

El‐Hack

et al. 2019

Animals

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Salmonellosis is one of the most important bacterial diseases in poultry, causing heavy economic losses, increased mortality and reduced production. The aim of this study was the comparative efficacy of a commercial probiotic and/or prebiotic with a live attenuated Salmonella Enteritidis (SE) vaccine on the protection of broiler chickens from SE challenge. The efficacy of probiotic or prebiotic products, as well as a live Salmonella Enteritidis (SE) vaccine at the 7th day of age, administered via drinking water, were evaluated for clinical protection and effects on growth performance of broiler chickens experimentally challenged with SE at the 28th day of age. The use of probiotic or prebiotic simultaneously with the live Salmonella vaccine can diminish the negative effect of live vaccine growth performance, reducing mortality rate, fecal shedding, and re-isolation of SE from liver, spleen, heart and cecum. The use of probiotic or prebiotic simultaneously with the application of the live Salmonella vaccine is a good practice to diminish the negative effect of the harmful bacteria and improve the growth performance of broilers. Thus, further studies may be carried out with layers and breeders.

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“…Increasing evidence indicates that Lactobacillus and Pediococcus strains exert beneficial effects by a variety of complementary mechanisms, including an ability to modulate host immune function, high adhesion capacity, cancerpreventing attributes, reduction of serum cholesterol levels, high antimicrobial activity against pathogens, and normalization of the microbiota composition (Okamoto et al, 2007;Noujaim et al, 2009; Isolation and Probiotic Potential of Lactobacillus salivarius and Pediococcus pentosaceus in Specific Pathogen Free Chickens Meng et al, 2010;Zhang et al, 2011;Kiran et al, 2015;Xie et al, 2015;Zhang et al, 2015). Promising probiotic strains should have some superior biological properties, especially improving growth performance, intestinal microbiota balance, and meat quality, and decreasing noxious gas emission (Meng et al, 2010;Fan et al, 2013;Jeong et al, 2014;Peng et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Isolation and Probiotic Potential of Lactobacillus Salivarius and Pediococcus Pentosaceus in Specific Pathogen Free Chickens

Chen

Zhu

Qiu

2017

Rev. Bras. Cienc. Avic.

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This study was conducted to isolate Lactobacillus salivarius and Pediococcus pentosaceus strains from cecal content and investigate their probiotic potential in specific pathogen free (SPF) chickens. L. salivarius and P. pentosaceus strains were isolated from the cecal content of SPF chickens and identified by 16s rDNA sequence analysis by BLAST analysis at the National Center for Biotechnology Information and phylogenetic analysis using DNAStar software. In an in vivo experiment, 180 7-day-old SPF chickens were randomly assigned into three groups. Group 1 served as a control that was fed a basal diet without probiotic supplementation, and groups 2 and 3 were fed the basal diets supplemented with L. salivarius and P. pentosaceus at 2×108 CFU/g, respectively. Body weight (BW), average daily gain (ADG), feed conversion ratio (FCR), dressing percentage (DP), and the apparent digestibility of crude protein (AD-CP) were calculated. We also determined meat color, fat content, shear force, water content and pH value of breast and thigh muscles; ammonia, urea nitrogen and uric acid content in plasma; fecal ammonia emission level and pH value; and Lactobacillus and Escherichia coli in ceca. Compared with the control group, L. salivarius and P. pentosaceus supplementation significantly increased BW, ADG, DP, AD-CP, fat content of meat, and the number of Lactobacillus in ceca (p<0.05), and decreased FCR, plasma ammonia content, fecal ammonia emission, and pH value and the number of E. coli in ceca (p<0.05). In the in vitro experiment, L. salivarius and P. pentosaceus treatments significantly decreased the ammonia content in medium compared with the control group without probiotic treatment (p<0.05). These results suggest that P. pentosaceus and L. salivarius strains show promising probiotic properties for improving growth, meat quality and microenvironment in chickens and decreasing ammonia content in the medium.

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Immunological evaluation of the intestinal mucosa of broiler chicks treated with Lactobacillus Spp. and challenged with Salmonella Enteritidis

Cited by 12 publications

References 6 publications

A Trial to Prevent Salmonella Enteritidis Infection in Broiler Chickens Using Autogenous Bacterin Compared with Probiotic Preparation

A Trial to Prevent Salmonella Enteritidis Infection in Broiler Chickens Using Autogenous Bacterin Compared with Probiotic Preparation

The Simultaneous Administration of a Probiotic or Prebiotic with Live Salmonella Vaccine Improves Growth Performance and Reduces Fecal Shedding of the Bacterium in Salmonella-Challenged Broilers

Isolation and Probiotic Potential of Lactobacillus Salivarius and Pediococcus Pentosaceus in Specific Pathogen Free Chickens

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