Immunological findings in whole and parotid saliva of patients with Crohn's disease and healthy controls

Crama-Bohbouth, G; P, Lems-van Kan; It, Weterman; Biemond, I.; Peña, A. S.

doi:10.1007/bf01317081

Cited by 18 publications

(16 citation statements)

References 5 publications

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“…This sug gestion is in agreement with the observation of a generally activated immunoglobulinsynthesizing system in CD in contrast with the local activated system in UC [5][6][7][8]. The relevance of these findings toward the patho genesis of IBD has still to be clarified in studies dealing with intestinal cells and 'spe cific' antigens.…”

Section: Discussionsupporting

confidence: 87%

“…Involvement of the immunoglobulin-syn thesizing system is supported by the in creased numbers of B cells [4] and plasma cells in the lamina propria of the colon in both CD and UC [5,6], Moreover, in CD other parts of the gastrointestinal tract also seem to take part in this process, since macroscopically unaffected buccal mucosa con tained increased numbers of immunoglobu lin-containing plasma cells, while saliva of CD patients had significantly elevated IgA, IgG and IgM levels compared with controls [7,8], The systemic activation of the immu noglobulin synthesis is suggested to be the result of the migration of committed lym phocytes from a site of antigenic stimulation to the mucosal tissue, including the secretory glands. This should be reflected in the in vitro response of (re)circulating lympho cytes.…”

Section: Introductionmentioning

confidence: 99%

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Differences in the Immunoglobulin Synthesis by Peripheral Blood Lymphocytes in Crohn’s Disease and Ulcerative Colitis

et al. 1987

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To determine whether patients with Crohn’s disease and ulcerative colitis dif-fere in their capacity to produce immunoglobulins, we have studied the in vitro immunoglobulin synthesis by peripheral blood lymphocytes of such patients and compared the results with those of healthy controls. The spontaneous immunoglobulin production by peripheral blood lymphocytes from Crohn’s disease patients was significantly increased for both IgA and IgG, whereas for IgM the increase did not reach statistical significance. In ulcerative colitis, however, the spontaneous IgA and IgG production was almost identical to that of the healthy controls and thus lower than in Crohn’s disease. Pokeweed mitogen stimulation resulted in a significantly enhanced immunoglobulin production in Crohn’s disease and in controls, whereas peripheral blood lymphocytes from ulcerative colitis patients were found to be rather insensitive to pokeweed mitogen stimulation. Suppression of the stimulated immunoglobulin production by concanavalin A revealed considerable reduction in all groups studied. In general, the highest suppression was found in patients with Crohn’s disease. In patients with Crohn’s disease or ulcerative colitis there was no relation between the changes in the in vitro immunoglobulin synthesis and the population of B or T lymphocytes and monocytes present in the peripheral blood. The differences in the spontaneous and stimulated immunoglobulin synthesis by peripheral blood lymphocytes, as found in this study, point to major changes in the regulation of the immunoglobulin synthesis in Crohn’s disease and ulcerative colitis.

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Section: Discussionsupporting

confidence: 87%

Section: Introductionmentioning

confidence: 99%

Differences in the Immunoglobulin Synthesis by Peripheral Blood Lymphocytes in Crohn’s Disease and Ulcerative Colitis

et al. 1987

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“…In summary, non‐specific IgA and IgA2 immunoglobulin levels in whole and parotid saliva are raised in patients with OFG, with or without gut inflammation, suggesting that salivary gland involvement may occur in this disorder. Although a previous study has reported raised salivary IgA in whole saliva in CD [28], no previous studies have compared OFG with and without gut involvment. Serum IgA responses to S. cerevisiae were raised only in those with existing gut disease and thus assay of serum IgA antibody responses may prove useful in indicating concurrent gut disease and possibly progression of OFG to gut involvment.…”

Section: Discussionmentioning

confidence: 99%

Serum and salivary IgA antibody responses to Saccharomyces cerevisiae, Candida albicans and Streptococcus mutans in orofacial granulomatosis and Crohn's disease

Savage

Barnard²,

Shirlaw³

et al. 2004

Clinical and Experimental Immunology

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Orofacial granulomatosis (OFG) is a condition of unknown aetiology with histological and, in some cases, clinical association with Crohn's disease (CD). However, the exact relationship between OFG and CD remains uncertain. The aim of this study was to determine whether OFG could be distinguished immunologically from CD by comparing non-specific and specific aspects of humoral immunity in serum, whole saliva and parotid saliva in three groups of patients: (a) OFG only (n = 14), (b) those with both oral and gut CD (OFG + CD) (n = 12) and (c) CD without oral involvement (n = 22) and in healthy controls (n = 29). Non-specific immunoglobulin (IgA, SigA, IgA subclasses and IgG) levels and antibodies to whole cells of Saccharomyces cerevisiae , Candida albicans and Streptococcus mutans were assayed by enzyme-linked immunosorbent assay (ELISA) in serum, whole saliva and parotid saliva. Serum IgA and IgA1 and IgA2 subclasses were raised in all patient groups (P < 0·01). Salivary IgA (and IgG) levels were raised in OFG and OFG + CD (P < 0·01) but not in the CD group. Parotid IgA was also raised in OFG and OFG + CD but not in CD. The findings suggest that serum IgA changes reflect mucosal inflammation anywhere in the GI tract but that salivary IgA changes reflect involvement of the oral cavity. Furthermore, the elevated levels of IgA in parotid saliva suggest involvement of the salivary glands in OFG. Serum IgA antibodies to S. cerevisiae were raised markedly in the two groups with gut disease while serum IgA (or IgG) antibodies to C. albicans were elevated significantly in all three patient groups (P < 0·02). No differences were found with antibodies to S. mutans. Whole saliva IgA antibodies to S. cerevisiae (and C. albicans) were raised in the groups with oral involvement. These findings suggest that raised serum IgA antibodies to S. cerevisiae may reflect gut inflammation while raised SIgA antibodies to S. cerevisiae or raised IgA or IgA2 levels in saliva reflect oral but not gut disease. Analysis of salivary IgA and IgA antibodies to S. cerevisiae as well as serum antibodies in patients presenting with OFG may allow prediction of gut involvement.

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“…Patients with IBD may complain of dry mouth, similar to the sicca syndrome observed in transplanted patients under immunosuppressive therapy. Interleukin‐6 changes in saliva of patients with IBD have been reported as well as several abnormal immunological findings in whole and parotid saliva …”

Section: Spectrum Of Oral Manifestations In Ibdmentioning

confidence: 99%

Review article: non‐malignant oral manifestations in inflammatory bowel diseases

Κατσάνος

Torres

Roda

et al. 2015

Aliment Pharmacol Ther

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Summary Background Patients with inflammatory bowel diseases (IBD) may present with lesions in their oral cavity. Lesions may be associated with the disease itself representing an extraintestinal manifestation, with nutritional deficiencies or with complications from therapy. Aim To review and describe the spectrum of oral nonmalignant manifestations in patients with inflammatory bowel diseases [ulcerative colitis (UC), Crohn's disease (CD)] and to critically review all relevant data. Methods A literature search using the terms and variants of all nonmalignant oral manifestations of inflammatory bowel diseases (UC, CD) was performed in November 2014 within Pubmed, Embase and Scopus and restricted to human studies. Results Oral lesions in IBD can be divided into three categories: (i) lesions highly specific for IBD, (ii) lesions highly suspicious of IBD and (iii) nonspecific lesions. Oral lesions are more common in CD compared to UC, and more prevalent in children. In adult CD patients, the prevalence rate of oral lesions is higher in CD patients with proximal gastrointestinal tract and/or perianal involvement, and estimated to range between 20% and 50%. Oral lesions can also occur in UC, with aphthous ulcers being the most frequent type. Oral manifestations in paediatric UC may be present in up to one‐third of patients and are usually nonspecific. Conclusions Oral manifestations in IBD can be a diagnostic challenge. Treatment generally involves managing the underlying intestinal disease. In cases presenting with local disabling symptoms and impaired quality of life, local and systemic medical therapy must be considered and/or oral surgery may be required.

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Immunological findings in whole and parotid saliva of patients with Crohn's disease and healthy controls

Cited by 18 publications

References 5 publications

Differences in the Immunoglobulin Synthesis by Peripheral Blood Lymphocytes in Crohn’s Disease and Ulcerative Colitis

Differences in the Immunoglobulin Synthesis by Peripheral Blood Lymphocytes in Crohn’s Disease and Ulcerative Colitis

Serum and salivary IgA antibody responses to Saccharomyces cerevisiae, Candida albicans and Streptococcus mutans in orofacial granulomatosis and Crohn's disease

Review article: non‐malignant oral manifestations in inflammatory bowel diseases

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