Immunological patterns of distribution of bacterial denitrifying enzymes

Michalski, Wojciech P.; Nicholas, D. J. D.

doi:10.1016/0031-9422(88)87011-0

Cited by 22 publications

(18 citation statements)

References 17 publications

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“…ELISA and Western blot analysis revealed that the molybdenum-containing nitrate reductase and the multi-copper nitrous oxide reductase appear to be common proteins among denitrifying bacteria. The pattern of immunological cross-reactivity for nitrite reductase, however, con-¢rmed that there are two di¡erent kinds of the enzyme, namely cd 1 NIR and Cu NIR, commonly distributed amongst bacteria [138]. The Cu NIR type from R. sphaeroides was also detected in Alcaligenes denitri¢cans, two Pseudomonas spp.…”

Section: Serological Approaches To Study Denitri¢cationmentioning

confidence: 99%

“…A DNA probe encoding cd 1 NIR showed a broader range of reactivity with different cd 1 NIR‐containing bacteria than did the serological results [137]. Michalski and Nicholas [138] used highly specific polyclonal antisera against the nitrate, nitrite, and nitrous oxide reductases of a photosynthetic, denitrifying bacterium ( Rhodobacter sphaeroides sp.f. denitrificans ) to investigate the distribution of bacterial denitrifying enzymes.…”

Section: Denitrificationmentioning

confidence: 99%

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Molecular analysis of ammonia oxidation and denitrification in natural environments

Bothe¹,

Jost²,

Schloter³

et al. 2000

FEMS Microbiol Rev

249

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This review summarizes aspects of the current knowledge about the ecology of ammonia-oxidizing and denitrifying bacteria. The development of molecular techniques has contributed enormously to the rapid recent progress in the field. Different techniques for doing so are discussed. The characterization of ammonia-oxidizing and -denitrifying bacteria by sequencing the genes encoding 16S rRNA and functional proteins opened the possibility of constructing specific probes. It is now possible to monitor the occurrence of a particular species of these bacteria in any habitat and to get an estimate of the relative abundance of different types, even if they are not culturable as yet. These data indicate that the composition of nitrifying and denitrifying communities is complex and apparently subject to large fluctuations, both in time and in space. More attempts are needed to enrich and isolate those bacteria which dominate the processes, and to characterize them by a combination of physiological, biochemical and molecular techniques. While PCR and probing with nucleotides or antibodies are primarily used to study the structure of nitrifying and denitrifying communities, studies of their function in natural habitats, which require quantification at the transcriptional level, are currently not possible.

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Section: Serological Approaches To Study Denitri¢cationmentioning

confidence: 99%

Section: Denitrificationmentioning

confidence: 99%

Molecular analysis of ammonia oxidation and denitrification in natural environments

Bothe¹,

Jost²,

Schloter³

et al. 2000

FEMS Microbiol Rev

249

View full text Add to dashboard Cite

show abstract

“…sphaeroides forma sp. denitrificans, 74 suggesting that they exhibit substantial structural similarity. Originally, many of these enzymes were reported to be dimers or tetramers of identical subunits with molecular weights in the 30-40 kDa range ( Table 2).…”

Section: B Copper-containing Nitrite Reductasesmentioning

confidence: 99%

Dissimilatory Nitrite and Nitric Oxide Reductases

1996

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“…denitrificans according to the procedures described previously [3][4][5]. These enzymes were used as antigens for the production of antibodies in rabbits as described in [3,11]. Specificity of purified lgG fractions was tested by a cross-immunoelectrophoresis technique [10] as well as by two-dimensional gel electrophoresis followed by Western immuno-blotting [11].…”

Section: Introductionmentioning

confidence: 99%

Identification of two new denitrifying strains of Rhodobacter sphaeroides

Michalski

1988

FEMS Microbiology Letters

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Highly specific polyclonal and antibodies against either nitrate, nitrite or nitrous oxide reductases from a photosynthetic denitrifying bacterium Rhodobacter sphaeroides f. sp. denitrificans were used to show the presence of immunologically reactive proteins in strains that Pellerin and Gest had shown to grow in the dark with nitrate as a terminal acceptor [9]. Two strains of this bacterium, namely 81‐3 and 2.4.3 synthesized the three denitrifying enzymes and were capable of denitrification. Strains 81‐1 and 2.4.1 (neotype) both expressed nitrate reductase activities but nitrite reductase was not detected since these strains did not reduce nitrite. They also did not grow in the dark with nitrate as a terminal acceptor. Each of strains 81‐1, 81‐3, 2.4.1 and 2.4.3 contain four plasmids. R. sphaeroides f. sp. denitrificans, however, contains only one large 108 kb plasmid, which is distinctly different in size from those detected in the other strains. This indicates that the 108 kb plasmid is not necessarily specific for denitrification.

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Immunological patterns of distribution of bacterial denitrifying enzymes

Cited by 22 publications

References 17 publications

Molecular analysis of ammonia oxidation and denitrification in natural environments

Molecular analysis of ammonia oxidation and denitrification in natural environments

Dissimilatory Nitrite and Nitric Oxide Reductases

Identification of two new denitrifying strains of Rhodobacter sphaeroides

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