Immunological rapid urease test using monoclonal antibody for <i>Helicobacter pylori</i>

Nakata, Hajime; Itoh, Hidekazu; Ishiguchi, Tadashi; Iwata, Tatsuya; Sato, Hiroaki; Higashimoto, Yuji; Fujimoto, Hisashi; Ichinose, Masakazu

doi:10.1111/j.1440-1746.2004.03403.x

Cited by 9 publications

(6 citation statements)

References 18 publications

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“…It provided an accuracy (sensitivity and specificity) comparable to those reported in previous studies in general practice [19,20].…”

Section: Discussionsupporting

confidence: 77%

“…Moreover, there was a significantly positive correlation between the pH of the IRUT and values for the UBT, which is widely used after eradication therapy against the infection [16,17]. Again, the IRUT is equally rapid (within 20 min) and less expensive compared to the UBT ($5 vs. $28 for reagent and $8200 vs. $15,600 for the apparatus, respectively) [20].…”

Section: Discussionmentioning

confidence: 96%

“…The IRUT was performed using a commercially available kit (HLS-2000; Olympus Optical Co.), as described previously [19,20]. In brief, the brush with the gastric mucus was washed in a dilution buffer (for each sample solution) and the solid-phase tip with a monoclonal antibody against H. pylori urease was introduced into the sample solution for 15 min at room temperature.…”

Section: Methodsmentioning

confidence: 99%

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Usefulness of the Immunological Rapid Urease Test for Detection of Helicobacter pylori in Patients Who are Reluctant to Undergo Endoscopic Biopsies

et al. 2006

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Cardiovascular diseases and liver cirrhosis are in the list of Helicobacter pylori-related extragastric diseases. Patients with cirrhosis and cardiovascular diseases under the control of aspirin have increased risk of gastrointestinal bleeding. The immunological rapid urease test (IRUT) provides a rapid and safe diagnostic test for H. pylori using gastric mucus collected at endoscopy. We investigated its usefulness in 93 patients with these extragastric diseases including 46 with H. pylori infection assessed by urea breath test and serology. Twenty H. pylori-infected patients received eradication therapy and the IRUT was assessed to evaluate the efficacy of bacterial eradication. The sensitivity, specificity, and positive and negative predictive values for IRUT were 96%, 90%, 90%, and 96%, respectively. The results of IRUT completely agreed with those of urea breath test following anti-H. pylori therapy. The IRUT has acceptable diagnostic performance in such cohorts reluctant to undergo endoscopic biopsies due to the risk of bleeding.

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“…It provided an accuracy (sensitivity and specificity) comparable to those reported in previous studies in general practice [19,20].…”

Section: Discussionsupporting

confidence: 77%

Section: Discussionmentioning

confidence: 96%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Usefulness of the Immunological Rapid Urease Test for Detection of Helicobacter pylori in Patients Who are Reluctant to Undergo Endoscopic Biopsies

et al. 2006

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“…Two new rapid urease tests (ProntoDry and HpONE) were found to have the same sensitivity as the CLO test, however, the new tests had faster reaction times [28,29]. A urease test using a monoclonal antibody against H. pylori urease was developed to increase both sensitivity and specificity [30].…”

Section: Molecular Methodsmentioning

confidence: 99%

Diagnosis of Helicobacter pylori Infection

2005

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“…The sensitivity of urease testing on antral biopsy specimens is 79-100%, and the specificity ranges from 92% to 100%. 27,28 However, an alternative diagnostic approach is in vivo identification of Helicobacter pylori using acriflavine-guided endomicroscopy. Helicobacter pylori infection was first detected with endomicroscopy in 2005 in a 70-year-old man.…”

Section: Helicobacter Pylorimentioning

confidence: 99%

Virtual histology

Kießlich¹,

Goetz²

2008

Best Practice & Research Clinical Gastroenterology

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Confocal laser endomicroscopy enables in vivo microscopy of the mucosal layer of the GI-tract with subcellular resolution during ongoing endoscopy. Endomicroscopy opens a new door for immediate tissue and vessel analysis. Different types of diseases can be diagnosed with optical surface and subsurface analysis. Analysis of the in vivo microarchitecture can be used for targeting biopsies to relevant areas. Furthermore, subsurface imaging can unmask microscopic diseases - (microscopic colitis) or bacterial infection (Helicobacter pylori), for example. Molecular imaging is becoming feasible, and this will shortly open the door to new indications in gastrointestinal endoscopy. This chapter reviews the currently rapidly expanding clinical data about endomicroscopy and gives a look into future research.

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Immunological rapid urease test using monoclonal antibody for Helicobacter pylori

Abstract: IRUT is a sensitive, specific and very rapid (within 20 min) method of detecting H. pylori infection.

Cited by 9 publications

References 18 publications

Usefulness of the Immunological Rapid Urease Test for Detection of Helicobacter pylori in Patients Who are Reluctant to Undergo Endoscopic Biopsies

Usefulness of the Immunological Rapid Urease Test for Detection of Helicobacter pylori in Patients Who are Reluctant to Undergo Endoscopic Biopsies

Diagnosis of Helicobacter pylori Infection

Virtual histology

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