Immunological Relationship Between Pernicious Anaemia and Thyroid Disease

Irvine, W. J.; Davies, S. H.; Delamore, I. W.; Williams, A. Wynn

doi:10.1136/bmj.2.5302.454

Cited by 159 publications

(40 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The findings presented in this paper extend the initial observations made by Irvine et al [1962] and fulfil the prophecy that the gastric antibody detected by complement-fixation is specific for the cytoplasm of parietal cells. It has also been confirmed that complement-fixing antibody is distinct from antibody to intrinsic factor, but in accordance with the findings of Taylor et al [1962] antibody to intrinsic factor does not appear to stain any other cell type in the mucosa of the body of human stomach.…”

Section: Disciussionsupporting

confidence: 74%

“…COMPLEMENT-FIXING antibodies to a saline extract of the mucosa of the stomach body were first described by Irvine et al [1962] in their study of the immunological relationship between Addisonian pernicious anaemia and thyroid disease. The relevant gastric antigen could only be extracted from the body of stomach and not from the pyloric antrum nor from lower levels in the alimentary tract.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Gastric Antibodies Studied by Fluorescence Microscopy

Irvine

1963

Exp Physiol

Self Cite

View full text Add to dashboard Cite

Antibody specific for the mucosa of the body of human stomach was detected by the method of complement-fixation in 62 per cent of patients with Addisonian pernicious anammia, in 20 per cent of patients with Hashimoto thyroiditis, in 25 per cent of patients with spontaneous hypothyroidism, and in 3 per cent of blood donors. Using the technique of fluorescence microscopy the specificity of this gastric antibody has been further defined to a particulate component of parietal cell cytoplasm. It is an auto-antibody that will react equally well with homologous gastric mucosa provided the mucosa contains a normal concentration of healthy parietal cells. The indirect fluorescent antibody technique is a more sensitive test for parietal cell antibody than the method of complementfixation and the incidence of positive results in Addisonian pernicious anoemia, Hashimoto thyroiditis, spontaneous hypothyroidism and blood donors is correspondingly higher at 78, 25, 32 and 4 per cent respectively. Complementfixing gastric antibody is distinct from antibody to intrinsic factor but the latter does not appear to stain any other cell type in the gastric body mucosa with the fluorescent antibody technique.The relationship between pernicious anaemia, chronic thyroiditis and idiopathic adrenal insufficiency is discussed and it is argued that one of the basic factors in the pathogenesis of these three conditions is a genetically determined defect in immunological tolerance.COMPLEMENT-FIXING antibodies to a saline extract of the mucosa of the stomach body were first described by Irvine et al. [1962] in their study of the immunological relationship between Addisonian pernicious anaemia and thyroid disease. The relevant gastric antigen could only be extracted from the body of stomach and not from the pyloric antrum nor from lower levels in the alimentary tract. The presence of gastric complement-fixing antibody in the serum of fourteen patients with spontaneous hypothyroidism was correlated with hypochlorhydria in response to an augmented histamine test. These two observations led to the conclusion that the occurrence of gastric antibodies could be either the result or the cause of damage to the parietal cell mass. The purpose of the present work was to study this gastric antigenantibody reaction in greater detail using the fluorescent antibody technique. MATERIALS AND METHODSThe sera studied were obtained from sixty-seven patients with proven Addisonian pernicious ansemia, 189 patients with either Hashimoto thyroiditis or spontaneous hypothyroidism and from 195 blood donors. While many of the patients with Addisonian pernicious an2emia or thyroid disease were newly diagnosed, others had received treatment for varying periods with parenteral cyanocobalamin or thyroxine.Both the direct and the indirect fluorescent antibody techniques of Coons and Kaplan [1950] have been employed. For the indirect method anti-human globulin conjugated with fluorescein (Progressive Laboratories, Baltimore) was used and for the direct method fluorescein iso...

show abstract

Section: Disciussionsupporting

confidence: 74%

mentioning

confidence: 99%

Gastric Antibodies Studied by Fluorescence Microscopy

Irvine

1963

Exp Physiol

Self Cite

View full text Add to dashboard Cite

show abstract

“…It has been suggested, however, on the basis of indirect evidence, that the mucosal glands in the body and fundus of the stomach may be destroyed by an autoimmune process (2). Antibodies to gastric intrinsic factor (3)(4)(5)(6) and to gastric parietal cell cytoplasmic antigen (2,(7)(8)(9)) may be present in sera from patients with pernicious anemia. Furthermore, treatment of pernicious anemia patients with prednisolone may result in a regeneration of chief and parietal cells in the gastric mucosa, with secretion of acid and intrinsic factor and improvement in vitamin B12 absorption (10,11).…”

mentioning

confidence: 99%

“…The distribution of this antibody in the immunoglobulin fractions of serum has not been determined, and the titer of antibody in serum has been measured only by a complement fixation reaction using crude gastric mucosal extracts as antigen (2,7,14).…”

mentioning

confidence: 99%

Studies of parietal cell antibody in pernicious anemia.

Jeffries¹,

Sleisenger²

1965

J. Clin. Invest.

View full text Add to dashboard Cite

“…There is extensive overlap between thyroid and gastric autoimmunity (Irvine et al 1962, Irvine 1965. It is not possible to separate these two tendencies by selecting first-degree relatives with only one type of antibody in their serum (Roitt & Doniach 1967).…”

Section: Genetic Aspectsmentioning

confidence: 99%

Autoimmunity in Endocrine Disease

Irvine

1980

Proceedings of the 1979 Laurentian Hormone Conference

View full text Add to dashboard Cite

Immunological Relationship Between Pernicious Anaemia and Thyroid Disease

Cited by 159 publications

References 4 publications

Gastric Antibodies Studied by Fluorescence Microscopy

Gastric Antibodies Studied by Fluorescence Microscopy

Studies of parietal cell antibody in pernicious anemia.

Autoimmunity in Endocrine Disease

Contact Info

Product

Resources

About