Immunological relationships during primary infection with Heligmosomoides polygyrus (Nematospiroides dubius): parasite specific IgG1 antibody responses and primary response phenotype

Wahid, Faisal; Behnke, Jerzy M.

doi:10.1111/j.1365-3024.1993.tb00625.x

Cited by 39 publications

(29 citation statements)

References 42 publications

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“…In this study, we have focussed on the helminth H. polygyrus , which is a natural parasite of the mouse, and to which different mouse strains show diverse patterns of susceptibility to infection, 36 , 38 with resistance clearly associating with strength of Th2 responsiveness 40 , 60 , 61 . Our new data highlight the significance of early events in infection, as by day 14 post inoculation, dramatic differences were apparent in worm fecundity between the strains, which preceded subsequent worm expulsion in the resistant genotypes.…”

Section: Discussionmentioning

confidence: 99%

Innate and adaptive type 2 immune cell responses in genetically controlled resistance to intestinal helminth infection

et al. 2014

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The nematode Heligmosomoides polygyrus is an excellent model for intestinal helminth parasitism. Infection in mice persists for varying lengths of time in different inbred strains, with CBA and C57BL/6 mice being fully susceptible, BALB/c partially so and SJL able to expel worms within 2–3 weeks of infection. We find that resistance correlates not only with the adaptive Th2 response, including IL-10 but with activation of innate lymphoid cell and macrophage populations. In addition, the titer and specificity range of the serum antibody response is maximal in resistant mice. In susceptible strains, Th2 responses were found to be counterbalanced by IFN-γ-producing CD4+ and CD8+ cells, but these are not solely responsible for susceptibility as mice deficient in either CD8+ T cells or IFN-γ remain unable to expel the parasites. Foxp3+ Treg numbers were comparable in all strains, but in the most resistant SJL strain, this population does not upregulate CD103 in infection, and in the lamina propria the frequency of Foxp3+CD103+ T cells is significantly lower than in susceptible mice. The more resistant SJL and BALB/c mice develop macrophage-rich IL-4Rα-dependent Type 2 granulomas around intestinal sites of larval invasion, and expression of alternative activation markers Arginase-1, Ch3L3 (Ym1) and RELM-α within the intestine and the peritoneal lavage was also strongly correlated with helminth elimination in these strains. Clodronate depletion of phagocytic cells compromises resistance of BALB/c mice and slows expulsion in the SJL strain. Thus, Type 2 immunity involves IL-4Rα-dependent innate cells including but not limited to a phagocyte population, the latter likely involving the action of specific antibodies.

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Section: Discussionmentioning

confidence: 99%

Innate and adaptive type 2 immune cell responses in genetically controlled resistance to intestinal helminth infection

et al. 2014

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“…Most previous studies (2,13) have examined effector responses in circulating blood, yet these may not reflect responses occurring at the intestinal site of nematode infection. For example, Ͼ99% of intestinal IgE raised against Trichinella spiralis is produced locally in the gut (14) and intestinal MMC responses to Hp are associated with arrested larval development and damage of young adult worms (15,16). In our study, the higher worm burdens in Hp-challenged mice fed low protein were associated with lower intestinal but not peripheral eosinophilia as well as diminished MMC proliferation and activation, thus supporting the concept that effector responses in GALT may be important during acquired resistance to nematode parasites.…”

Section: Discussionmentioning

confidence: 99%

Suppressed T helper 2 immunity and prolonged survival of a nematode parasite in protein-malnourished mice

Ing

Scott

et al. 2000

Proc. Natl. Acad. Sci. U.S.A.

112

106

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“…The same pools of reference sera were employed throughout this work. See Wahid & Behnke (1993 16), for a full description.…”

Section: Methodsmentioning

confidence: 99%

Cellular and serological responses in resistant and susceptible mice exposed to repeated infection with Heligmosomoides polygyrus bakeri

Behnke

Lowe

Clifford

et al. 2003

Parasite Immunology

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An experiment was carried out to compare the parasitological and immunological responses of SWR and CBA mice to trickle (repeated) infection with Heligmosomoides polygyrus. Male mice were given 125 L3 once per week and were killed in groups, together with naïve control mice, weekly until week 8. Worm burdens accumulated in CBA, stabilizing in week 5 in excess of 400 worms and remaining high until week 8. In contrast in SWR worm burdens peaked in week 3 at a mean worm burden of 129 and then fell sharply so that by week 6, despite continuing re-infection, no more worms were recovered from these mice. SWR mice showed a marked mast cell and mMCP-1 response, peaking in weeks 2-3, whereas in CBA mice these responses were slower, and even at their height in week 8 still less intense than those in SWR mice. Both strains responded initially with a very similar goblet cell response, which declined in SWR mice as worms were eliminated, but was sustained in CBA mice until week 8. Serum TNFalpha concentrations were higher in SWR mice throughout the experiment. Infection elicited strong serological responses against adult and L4 antigens in both SWR and CBA mice, involving all the isotypes tested (IgG1, IgA and IgE). Anti-L3 responses were examined only for IgG1. However, only two responses differed significantly between the strains: the IgE response to L4 antigens was more intense in SWR mice, and interestingly and unexpectedly, the IgG1 response to adult worm antigens was more intense in CBA mice. These results reflect the activation of predominantly Th2-driven effector mechanisms, that may be associated with host-protective immunity developing under the trickle infection protocol exploited in these experiments.

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Immunological relationships during primary infection with Heligmosomoides polygyrus (Nematospiroides dubius): parasite specific IgG1 antibody responses and primary response phenotype

Abstract: SUMMARYIgGl antibody responses to Heligmosomoides polygyrus were measured in eight mouse strains supporting acute (

Cited by 39 publications

References 42 publications

Innate and adaptive type 2 immune cell responses in genetically controlled resistance to intestinal helminth infection

Innate and adaptive type 2 immune cell responses in genetically controlled resistance to intestinal helminth infection

Suppressed T helper 2 immunity and prolonged survival of a nematode parasite in protein-malnourished mice

Cellular and serological responses in resistant and susceptible mice exposed to repeated infection with Heligmosomoides polygyrus bakeri

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