Immunological studies of<i>Morinda officinalis</i>: How polysaccharides act as adjuvants

Feng, Haibo; Zhi, Hui; Hu, Xin; Yang, Yan; Zhang, Linzi; Liu, Qianqian; Feng, Yangyang; Wu, Daiyan; Yang, Xiaoyan

doi:10.1080/07328303.2021.1954657

Cited by 2 publications

(2 citation statements)

References 44 publications

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“…[ 25 ]. Moreover, MOP has been used as an immunopotentiator [ 26 ] and leukopenia treatment in mice [ 27 ]. Furthermore, the MOP has numerous active hydroxyl groups and excellent water solubility with non-toxicity.…”

Section: Introductionmentioning

confidence: 99%

Selenium Nanoparticles Based on Morinda officinalis Polysaccharides: Characterization, Anti-Cancer Activities, and Immune-Enhancing Activities Evaluation In Vitro

et al. 2023

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Recently, selenium nanoparticles have been drawing attention worldwide, and it is crucial to increase the stability of nano-Se. Morinda officinalis polysaccharides (MOP) are the main active component in Morinda officinalis radix. However, their low activity has limited their application. A novel selenium nanoparticle (Se-MOP) was prepared to solve these problems using MOP as a dispersant. The zeta potential was measured to evaluate the stability, and UV and ATR-FTIR were used to investigate the binding type of selenium and MOP. The morphology was observed by the TEM method. Furthermore, the inhibitory effect on five selected cancer cells (HepG2, MCF-7, AGS, PC9, and HCT8) was evaluated, showing remarkable inhibition of all five cancer cells. The mechanism of inhibition was also investigated by cell circle assay, and it was found that Se-MOP could induce cell circle G0/G1 phase arrest. Immune-enhancing activities were evaluated by measuring the proliferation and cytokines of mouse spleen lymphocytes in vitro and quantitative RT-PCR. The results indicated that single stimulation of Se-MOP and synergistic stimulation with PHA or LPS increased immune capacity and improved immune by increasing the expression of cytokines.

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Section: Introductionmentioning

confidence: 99%

Selenium Nanoparticles Based on Morinda officinalis Polysaccharides: Characterization, Anti-Cancer Activities, and Immune-Enhancing Activities Evaluation In Vitro

et al. 2023

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“…[7] In recent years, polymer materials have attracted extensive attention in the field of vaccine adjuvants due to their good biological safety, biocompatibility, and because they are not easily decomposed by enzymes in the body. Commonly used polymer materials mainly include polylactic acid, [8] polysaccharides, [9] and polyacrylate. [10] Among these, polyacrylate materials have significant advantages when used as vaccine adjuvants because of their low price, simple preparation process, and easy-to-scale production.…”

Section: Introductionmentioning

confidence: 99%

Preparation and Evaluation of Polyacrylate Microgels and Their Adjuvant Activities Using Ovalbumin as a Model Antigen

Zhou

et al. 2023

ChemistryOpen

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As vaccine adjuvants, polyacrylate materials can induce a specific immune response in the body and have been widely studied in recent years due to their advantages, such as their safety, effectiveness, and low required dosage. In this study, a series of polyacrylates with hydrophobic physical crosslinking and chemical crosslinking were prepared using precipitation polymerization, and their structures were characterized by nuclear magnetic resonance spectroscopy and Fourier‐transform infrared spectroscopy. The optimal reaction conditions were determined according to the effect of reaction time, azodiisobutyronitrile, Span 60, allyl pentaerythritol, and octadecyl methacrylate (OMA) contents on the viscosity of the polyacrylate microgel, combined with the effects of allyl pentaerythritol and OMA contents on the subcutaneous immune safety of the polyacrylate microgel in BALB/c mice. The polyacrylate microgels with different OMA contents showed good biological safety. In addition, in vivo immunity experiments were carried out in mice to analyze the adjuvant properties of ovalbumin as a model antigen. Based on the titer results of the IgG1 and IgG2a antibodies, with 1 wt % OMA content, the polyacrylate microgel vaccine could optimally induce the body to produce an immune response type dominated by Th2‐type humoral immune response and supplemented by Th1‐type cellular immune response.

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Immunological studies ofMorinda officinalis: How polysaccharides act as adjuvants

Cited by 2 publications

References 44 publications

Selenium Nanoparticles Based on Morinda officinalis Polysaccharides: Characterization, Anti-Cancer Activities, and Immune-Enhancing Activities Evaluation In Vitro

Selenium Nanoparticles Based on Morinda officinalis Polysaccharides: Characterization, Anti-Cancer Activities, and Immune-Enhancing Activities Evaluation In Vitro

Preparation and Evaluation of Polyacrylate Microgels and Their Adjuvant Activities Using Ovalbumin as a Model Antigen

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