Immunological Studies on Experimental Infection of Pigs withAscaris suumGoeze, 1782 I. An Introduction with a Review of the Literature and the Demonstration of Complement-fixing Antibodies in the Serum

Abstract

1. An introduction to a study of immunity againstA. suumin the pig is given. A review of the literature on the life cycle, a note on terminology and a general discussion on immunity to helminths has also been included.2. By first removing the procomplementary activity of pig serum to guineapig complement with hydrochloric acid, it was possible to apply the complement fixation test to a study of the antibody response to experimental infection withA. suum.3. Using an alcoholic extract of uterus, oviducts and ovaries of the adult femaleAscarisworm as antigen, antibodies against this nematode were first detected in two experimentally infected pigs ten days after infection. A peak of antibody concentration from the thirteenth to the seventeenth day of infection was followed by a decline in the antibody content of the serum to the forty-first day; after which no antibody was detected.

Immunological Studies on Experimental Infection of Guineapigs and Rabbits with Ascaris suum Goeze, 1782. I.—The Minimum Lethal Dose (M.L.D.) and the Minimum Immunizing Dose (M.I.D.) of Ascaris Eggs in Guineapigs

“…The object of the present investigation, a preliminary to immunological studies in the natural host, the pig (part already published, Taffs, 1961b, c ;1962 ;1963 ;1964a, b, c), was to confirm and extend the researches of previous workers. In the present paper the minimal lethal dose (M.L.D.)…”

Immunological Studies on Experimental Infection of Pigs with Ascaris suum Goeze, 1782. II. The Use of the Conglutinating Complement Absorption Test for the Demonstration of Ascaris Antibody

“…It has been shown that complement-fixing antibodies can be detected in the serum of pigs infected with A. suum, after the removal of the procomplementary activity of pig serum with hydrochloric acid (Taffs, 1961). This paper describes the use of the conglutinating complement absorption test (C.C.A.T.…”

Immunological Studies on Experimental Infection of Pigs withAscaris suumGoeze, 1782. VI. The Histopathology of the Liver and Lung

“…G). It has been shown in previous work (Taffs, 1961c ;1964b) that complement-fixing antibodies can be detected in the circulation ten days after infection ; there is no reason to assume, however, that these or other antibodies-possibly precipitins-are not present in the tissues by the seventh day. If the material which is found around larvae represents a union of antigen with antibody, then this reaction may explain why some larvae which were found in the lungs of resistant animals were not surrounded by inflammatory cells.…”