Immunological studies on LATS-immunoglobulin by the reaction with staphylococcal protein A.

Abstract

The reaction of LATS activity with Staphylococcal Protein A, a specific binding protein with the Fc part of human IgG(1), IgG(2) and Ig(4), was examined. When IgG(1), IgG(2) and Ig(4) subclasses were removed from LATS positive sera or LATS-IgG fractions by affinity chromatography on Protein A-Sepharose, LATS activity decreased. Almost all LATS activity was found in the fraction that reacted with Protein A. It is suggested that LATS has an expression of a very distinct immunoglobulin G structure, and that LATS activity is distributed mainly in the fraction containing IgG(1), Ig(2) and Ig(4) in LATS positive serum;

Neutralization and purification of thyroid stimulating antibody (TSAb) and thyroid blocking antibody (TBAb) by heterophilic antibody to animal IgG in Graves' disease

“…Protein A method [23]. TSAb-IgG was applied to the affinity column (1.5 X5 cm) and incubated 30 min.…”

Distribution of Lats Activity in Immunoglobulin G Subclass

“…In a previous experiment, we reported that LATS activity was dis¬ tributed in the fraction containing the 3 subclasses, IgG(l), IgG(2) and LG(4) (Ochi et al 1976). The present paper examines the separation of LATSimmunoglobulin from LATS positive serum using affinity chromatography on Anti-IgG, Anti-Fab, Anti-Fc, and Staphylococcal Protein A bound Sepharose, and the immunological properties of the biologically active fragment in LATSimmunoglobulin after hydrolysis.…”

Interaction of Thyroid-Stimulating Antibody with Graves’ Thyroid-Stimulating Hormone-Binding Antibody

“…TSAb-IgG (by Protein A method [14]) was purified from other Graves’ serum with high TSAb activities. The crude IgG obtained by PEG 13.5% precipitated fraction (PF) from serum (0.2 ml) of patients with both anti-bTSH antibody and no TSAb activity was dissolved with modified Hanks’ buffer (TSAb kit).…”

“…All fractions which had been lyophilized after dialysis against distilled water for 3 days at 5°C were used for TSAb assay. An F(ab′) 2 fragment from other Graves’ TSAb-IgG was obtained from pepsin-digested TSAb-IgG using Protein A-Sepharose as previously reported [14, 15]and was affinity-purified.…”