Immunological studies on Vibrio anguillarum

Johnsen, Geir

doi:10.1016/0044-8486(77)90003-5

Cited by 31 publications

(19 citation statements)

References 12 publications

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“…The numerous isolates of V. anguillarum have been characterized and classified according to their genetical, biochemical and serologieal properties. Different international serologieal classification systems are used (Johnsen 1977;Kitao, Aoki, Fukudome, Kawano, Wada & Mizuno 1983;Tajima, Ezura & Kimura 1985;Skov S0rensen & Larsen 1986). Serotyping gives valuable information about antigenic similarities and differences between bacteria, which is of major importance for the development of effective vaccines.…”

Section: Introductionmentioning

confidence: 99%

Vaccination experiments and studies of the humoral immune responses in cod, Gadus morhua L., to four strains of monoclonal‐defined Vibrio anguillarum

Espelid

Rødseth

Jørgensen

1991

Journal of Fish Diseases

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Abstract. Vibrio anguillarum isolated from diseased cod, Gadus morhua L., were serotyped by use of a panel of monoclonal antibodies. Four serotypes could be distinguished, having different lipopolysaccharide determinants. These phenotypic differences were also reflected in the genetic map, as revealed by fingerprinting of bacterial DNA. Antisera were raised in cod after immunization with the V. anguillarum serotypes, and Western blot techniques demonstrated production of specific antibodies mainly to LPS‐antigens. The immune system in cod discriminates to a eertain degree between the four serotypes as shown by crossreactions of the immune sera in elisa. Moreover, it was also shown that natural antibodies to bacterial antigens are present in non‐immune sera, but these specificities are non‐LPS in nature. As a consequence of the heterogeneity of the V. anguillarum strains, vaccination experiments were performed under laboratory conditions to compare the effectiveness of bacterins based on either single vaccines or polyvaccines. The results from these experiments were promising since challenge with one strain demonstrated 100% protection both in fish vaccinated with the homologous serotype as well as a mixture of all the four serotypes.

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Section: Introductionmentioning

confidence: 99%

Vaccination experiments and studies of the humoral immune responses in cod, Gadus morhua L., to four strains of monoclonal‐defined Vibrio anguillarum

Espelid

Rødseth

Jørgensen

1991

Journal of Fish Diseases

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“…Since then, several serotyping systems based on the slide agglutination method with thermostable 0 antigens have been developed. Up to 1986, 3 serotypes were recognized in Norway (Johnsen 1977), 2 or 3 in the United States (Harrell et al 1976, Strout et al 1978, and 9 in Japan (Kitao et al 1984). Ssrensen & Larsen (1986) recommended an international harmonization of the diagnostic efforts and presented a new antigenic typing scheme in which 10 distinct 0 serotypes of V. anguillarum were recognized.…”

Section: Serologymentioning

confidence: 99%

A review of the taxonomy and seroepizootiology of Vibrio anguillarum, with special reference to aquaculture in the northwest of Spain

Toranzo¹,

Barja²

1990

Dis. Aquat. Org.

135

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ABSTRACT. A review of the literature shows that although the number of serotypes of Vibrio anguillarum reported from different countries varies, most of the vibriosis outbreaks throughout the world are caused by only 2 serotypes: 0 1 and 0 2 (European serotype designation). The remaining serotypes, associated mainly with environmental samples such as water, sediment, phyto-and zooplankton, are generally non-pathogenic to fish. This raises the question as to whether the 2 major pathogenic serotypes of V. anguillarum (isolated only from diseased or carrier fish) should be considered opportunistic or obligate fish pathogens. In addition, information useful in epizootiological and vaccination studies is presented about the antigenic heterogeneity detected within serotype 0 2 . In this review, emphasis is placed on the presence in the marine environment of vibrios (presumptively assigned to the V. splendidus and V. pelagius) species that are taxonomically and serologically related with V. anguillarum and some of which have been associated with disease in fish cultured on the Atlantic coast of Spain and Norway. The precise phylogenetic position of these V. anguillarum-like organisms are well as their actual threat to marine aquaculture remains to be clarified.

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“…Various microorganisms and their components, particularly bacterial LPS, are known to exhibit these activities (6,23,28). Johnsen extracted LPS from V. anguillarum by the hot phenol-water method and serological groups were determined according to the type of LPS in the cell wall (15), but their chemical characteristics and biological activities were not examined.…”

Section: Acid Phosphatase Actioity In Peritoneal Exudate Cellsmentioning

confidence: 99%

“…At present, V. anguillarum is considered to have at least three different 0 serotypes (1, 15,21). A difference in the neutral sugar composition in whole cells of the three serotype strains was detected by gas-liquid chromatography (manuscript in preparation).…”

Section: Acid Phosphatase Actioity In Peritoneal Exudate Cellsmentioning

confidence: 99%

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Mitogenicity and Adjuvanticity of a Marine Bacterium, Vibrio anguillarum, in Mice

Shimizu

Nitta

Itoh

et al. 1981

Microbiology and Immunology

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The effects of whole cells of three different 0 serotypes of Vibrio anguillarum on the murine immune response were studied. The addition of different doses (1-100 p,g/ml) of V. anguillarum cells, as well as Salmonella typhimurium lipopolysaccharide, markedly increased the incorporation of [3H]thymidine into in vitro cultured spleen cells of C57BL/6 mice. All three serotype strains of V. anguillarum were able to induce the mitogenic effect at 10 p,g/ml and 100 p,g/m1, but serotype I strains were more potent than the others. Since pretreatment of spleen cells with rabbit anti-mouse thymocyte antiserum did not affect the mitogenic activity of V. anguillarum, Vibrio cells may be a B-lymphocyte mitogen.When sheep or horse erythrocytes and Vibrio cells were injected intraperitoneally into ddY mice, Vibrio cells exhibited an enhancing effect on antibody response in vivo, regardless of the different serotypes. Vibrio cells, when injected intraperitoneally into mice before the antigen, markedly suppressed the antibody response. Several days after the injection of Vibrio cells, these mice showed an enhanced carbon clearance activity. Acid phosphatase activity in their peritoneal cells was also augmented, suggesting that Vibrio cells activated macrophages in the mice.Vibrio anguillarum, a gram-negative and halophilic marine bacterium, causes vibriosis in fish, both wild and cultured (I, 17,25); this is one of the most serious diseases affecting different kinds of fish (25).We previously reported that V. anguillarum has antitumor activity against Ehrlich carcinoma cells in mice, which may be mediated by immune lymphocytes of the host (24). Although numerous physiological, biochemical and serological studies on V. anguillarum have been carried out (1,15,17,21,25), no report has been published on the effect of the bacteria on the host immune systems.In this paper, we describe the following effects of whole V. anguillarum cells of three different 0 serotypes: a) a mitogenic effect on splenocytes (3[H]thymidine uptake), b) an adjuvant effect on antibody response to xenogeneic erythrocyte antigen, c) stimulation of the carbon clearance system, and d) enhancement of acid phosphatase activity in peritoneal exudate cells.929

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Immunological studies on Vibrio anguillarum

Cited by 31 publications

References 12 publications

Vaccination experiments and studies of the humoral immune responses in cod, Gadus morhua L., to four strains of monoclonal‐defined Vibrio anguillarum

Vaccination experiments and studies of the humoral immune responses in cod, Gadus morhua L., to four strains of monoclonal‐defined Vibrio anguillarum

A review of the taxonomy and seroepizootiology of Vibrio anguillarum, with special reference to aquaculture in the northwest of Spain

Mitogenicity and Adjuvanticity of a Marine Bacterium, Vibrio anguillarum, in Mice

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