Immunological study of the glial fibrillary acidic protein

Uyeda, Charles T.; Eng, Lawrence F.; Bignami, A.

doi:10.1016/0006-8993(72)90347-2

Cited by 176 publications

(43 citation statements)

References 12 publications

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“…The apparent selectivity of the antisera for the astroglial component could be explained with the fact that the original preparations used as antigen were isolated from tissue selectively enriched in glial fibers (multiple sclerosis plaques) [16,17]. However, we have recently prepared antisera Fig.2.…”

Section: Resultsmentioning

confidence: 99%

Isolation from peripheral nerve of a protein similar to the glial fibrillary acidic protein

Dahl

Bignami

1976

FEBS Letters

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Section: Resultsmentioning

confidence: 99%

Isolation from peripheral nerve of a protein similar to the glial fibrillary acidic protein

Dahl

Bignami

1976

FEBS Letters

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“…GFA preparation.-GFA was partially purified by ammonium sulphate precipitation (Uyeda, Eng and Bignami, 1972) using sheep and human brains obtained within 12 h of death.…”

Section: Methodsmentioning

confidence: 99%

Glial fibrillary acidic protein in tumours of the nervous system

Delpech¹,

Delpech²,

Vidard³

et al. 1978

Br J Cancer

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Summary.-Glial fibrillary acidic protein (GFA) was assayed in nerve-tumour extracts and located in these tumours by indirect immunofluorescence study. We conclude that GFA is a specific marker of both malignant and normal astrocytes. Non-astrocytic tumours (oligodendroglioma, meningioma) do not contain GFA. Tumours with astrocytic differentiation potential (medulloblastoma) may contain GFA. Comparison of microscopic and GFA assays leads us to conclude that GFA concentration is proportional to the amount of malignant astrocytes in the tumour and inversely proportional to the necrotic area of a tumour. Normal tissue GFA and glioblastoma GFA were found to be immunologically identical.

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“…However, this protein is also found in the pyramidal tracts on the ninth postnatal day of rats at the time of myelination gliosis and the cell bodies of the ependymal cells did not stain for the glial fibrillary acidic protein (MORI et al, 1968;UYEDA et al, 1972;WICKREMSINGHE, 1972;BIGNAMI et al, 1972BIGNAMI et al, , 1973LUDWIN et al, 1977;BOCK, 1978;SCHMECHEL et al, 1978). Our glial specific protein was different from the glial fibrillary acidic protein and other proteins on the basis of electrophoretic mobility and cellular localization.…”

Section: Discussionmentioning

confidence: 73%

“…On the basis of their topography and morphology the Muller cells have been described as possessing the nature of both ependymal and glial cells (WOLTER, 1959;LADMAN and SOPER, 1962;HOSOKAWA and MANNEN, 1963;HANSSON, 1971 are usually considered of glial origin (MORI et al, 1968;UYEDA et al 1972;WICKREM-SINGHE, 1972;BIGNAMI et al, 1972BIGNAMI et al, , 1973DELAUNOY et al, 1977;LUDWIN et al, 1977;BOCK, 1978;SCHMECHEL et al, 1978). By immunofluorescence techniques with anti-glial fibrillary acidic protein serum, BIGNAMI et al (1972) found a selective staining of fibrous astrocytes and their processes.…”

Section: Discussionmentioning

confidence: 99%