Immunological tumour profile: Organ-specific carcinoma diagnosis in patients employing the macrophage electrophoretic mobility test

Immunodiagnostics of Malignant Disease VI. Electrophoretic Mobility Test (EMT) in Malignant Melanoma

“…Recently tumor specific or tumor associated antigens have been demonstrated in several tumor types [16,19,25]. The tumor antigens are located either intracellularly or in the cell membrane, and induce specific humoral or cellular immune reactions [6, 9, 12, 13.…”

“…MOller et at. isolated tumor antigens by KCI extraction and demonstrated a reaction only with lymphocytes of patients whose tumor was histologically identical to that from which the antigen was isolated [24,25], In the case of melanoma Jehn et al showed a soluble antigen which gava a specific reaction in Similar results were obtained by Cochran et al, using a soluble melanoma antigen in a leucocyte-migration-inhibition tion assay [2], This study shows a tumor specific reaction in melanoma pa tients using a 3M KCI extract from melanoma tissue as antigen in the EMT.…”

Cell electrophoresis for diagnostic purposes. I. Diagnostic value of the electrophoretic mobility test (EMT) for the detection of gynaecological malignancies

“…Several centres (using EF, CaBP or the KCI extracts as "antigens") confirmed the results of MEM (Pritchard et al, 1972(Pritchard et al, , 1973Preece & Light, 1974;Field, 1976;Irmscher et al, 1975;Klausch et al, 1975;Meyer-Rienecker et al, 1975;Jenssen et al, 1976a,b;Nowak et al, 1976;Klausch et al, 1977;Light & Preece, 1977;Muller et al, 1977;Gunther et al, 1978), MOD-MEM (Pritchard et al, 1976) and EMT (Jenssen & Shenton, 1975;Lampert et al, 1977;Shenton et al, 1977;Douwes et al, 1978;Dyson & Corbett, 1978;Tautz et al, 1978;Ritter & Oehme, 1978;Kreienberg et al, 1979;B6gelspacher et al, 1980), though the procedures have not gained universal recognition (see also Bagshawe, 1973;leading article in Nature, 1973;editorial in Lancet, 1976;Bagshawe, 1977;Moore & Lajtha, 1977;Moore, 1978).…”

“…After a small and at first confirmatory test series in 1973 (Goldstone et al, 1973), Lewkonia et al (1974) Rahi et al (1976), Rawlins et al (1976), Arvilommi et al (1977), Chiu et al (1977 and Nakajima et al (1977) rejected the test because of its inability to distinguish between subjects with malignant disease and the controls, others have criticized the theoretical basis of the test system (Forrester et al, 1977). Shenton et al (1977) found, in a mutual study with the Rostock team, a positive concordance of the EMT and MEM results and a high correlation with clinical diagnosis, but Harlos & Weiss (1978) failed to do so; only 23 of the 42 samples analysed showed agreement between the two systems.…”

Interaction of myelin basic protein with mononuclear cells: The primary reaction for the MEM and EMT tests

“…Brain extract, homogeneous preparations of myelin basic protein MBP (= encephalitogenic factor EF) or extracts from cancer tissue have been used as "antigens" (e.g. Dyson & Corbett, 1978;Muller et al, 1977); macrophages as indicator cells have been substituted by sheep erythrocytes tanned and stabilized with sulphosalicylic acid (ETS) (Porzsolt et al, 1975). Whatever the modifications, the results are contradictory: some investigators claim to detect differences in the response of healthy subjects and cancer patients; others cannot reproduce these findings (reviews by Porzsolt, 1978;Mross & Wolfrum, 1980 (Dyson, 1979;Schumacher & Grol, 1981).…”