Immunological uterine response to pig embryos before and during implantation

Parrilla, Inmaculada; Gil, M.A.; Cuello, C.; Cambra, Josep M.; Plaza, Alejandro Gonzalez; Lucas, Xiomara; Vázquez, José Luís; Vázquez, Juan M.; Rodriguez‐Martinez, Heriberto; Rodríguez‐Martínez, H.

doi:10.1111/rda.14142

Cited by 10 publications

(7 citation statements)

References 63 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The unique proteins involved in the immune system of PS are of particular interest, given that the modulation of the endometrial immune system is crucial for endometrial function and implantation [ 9 , 22 ]. Among the 53 PS-unique proteins annotated, 10 proteins were involved in cytokine signaling in immune system pathway in ClueGo analysis.…”

Section: Discussionmentioning

confidence: 99%

“…Research has shown that the mid-implantation stage (d 18) is characterized by an increase in pro-inflammatory cytokine expression, which facilitates embryo implantation interactions between the placenta and uterus during porcine pregnancy [ 23 , 24 ]. Conversely, during the late implantation phase (d 20–28), anti-inflammatory expression is observed to support placental and conceptus development [ 22 ]. Practically all identified PS-unique proteins involved in immune system process were pro-inflammatory proteins, with the exception of HMOX1, which has anti-inflammatory properties.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

In-depth proteome characterization of endometrium and extraembryonic membranes during implantation in pig

Gil,

Cambra,

Rodriguez-Martinez

et al. 2024

J Animal Sci Biotechnol

Self Cite

View full text Add to dashboard Cite

Background Proteome characterization of the porcine endometrium and extraembryonic membranes is important to understand mother-embryo cross-communication. In this study, the proteome of the endometrium and chorioallantoic membrane was characterized in pregnant sows (PS) during early gestation (d 18 and 24 of gestation) and in the endometrium of non-pregnant sows (NPS) during the same days using LC-MS/MS analysis. The UniProtKB database and ClueGO were used to obtain functional Gene Ontology annotations and biological and functional networks, respectively. Results Our analysis yielded 3,254 and 3,457 proteins identified in the endometrium of PS and NPS, respectively; of these, 1,753 being common while 1,501 and 1,704 were exclusive to PS and NPS, respectively. In addition, we identified 3,968 proteins in the extraembryonic membranes of PS. Further analyses of function revealed some proteins had relevance for the immune system process and biological adhesion in endometrium while the embryonic chorion displayed abundance of proteins related to cell adhesion and cytoskeletal organization, suggesting they dominated the moment of endometrial remodeling, implantation and adhesion of the lining epithelia. Data are available via ProteomeXchange with identifier PXD042565. Conclusion This is the first in-depth proteomic characterization of the endometrium and extraembryonic membranes during weeks 3 to 4 of gestation; data that contribute to the molecular understanding of the dynamic environment during this critical period, associated with the majority of pregnancy losses.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

In-depth proteome characterization of endometrium and extraembryonic membranes during implantation in pig

Gil,

Cambra,

Rodriguez-Martinez

et al. 2024

J Animal Sci Biotechnol

Self Cite

View full text Add to dashboard Cite

show abstract

“…The H&E staining results showed dynamic changes in the placental structure across p21, p28, and p35. Embryo attachment is completed on day 18 in pigs, and then, the placenta begins to form [ 36 ]. Our study showed that trophoblast thickness constantly increased from p21 to p35.…”

Section: Discussionmentioning

confidence: 99%

Integrated Analysis of DNA Methylation and Gene Expression in Porcine Placental Development

Tan

Zhou

Zang

et al. 2023

IJMS

View full text Add to dashboard Cite

Proper placental development is crucial for the conceptus to grow and survive, because the placenta is responsible for transporting nutrients and oxygen from the pregnant female to the developing fetus. However, the processes of placental morphogenesis and fold formation remain to be fully elucidated. In this study, we used whole-genome bisulfite sequencing and RNA sequencing to produce a global map of DNA methylation and gene expression changes in placentas from Tibetan pig fetuses 21, 28, and 35 days post-coitus. Substantial changes in morphology and histological structures at the uterine–placental interface were revealed via hematoxylin–eosin staining. Transcriptome analysis identified 3959 differentially expressed genes (DEGs) and revealed the key transcriptional properties in three stages. The DNA methylation level in the gene promoter was negatively correlated with gene expression. We identified a set of differentially methylated regions associated with placental developmental genes and transcription factors. The decrease in DNA methylation level in the promoter was associated with the transcriptional activation of 699 DEGs that were functionally enriched in cell adhesion and migration, extracellular matrix remodeling, and angiogenesis. Our analysis provides a valuable resource for understanding the mechanisms of DNA methylation in placental development. The methylation status of different genomic regions plays a key role in establishing transcriptional patterns from placental morphogenesis to fold formation.

show abstract

“…The implantation of an embryo for pregnancy initiation represents the most important stage in the onset of pregnancy and involves a process of superimposition of a blastocystderived signature onto the receptive endometrium during the mid-secretory phase of the menstrual cycle, the so-called "window of receptivity". Both a receptive endometrium and timely signaling between the blastocyst and endometrium determine the success of implantation [17]. For instance, such endometrial factors as calcitonin [18], lysophosphatidic acid (LPA) [19], heparin-binding epidermal growth factor (HB-EGF) [20], leukemia-inhibiting factor (LIF) [21], and epidermal growth factor (EGF) [16] promote embryo implantation.…”

Section: Discussionmentioning

confidence: 99%

Local Immune Biomarker Expression Depending on the Uterine Microbiota in Patients with Idiopathic Infertility

Tapilskaya

Savicheva

Shalepo

et al. 2023

IJMS

View full text Add to dashboard Cite

The endometrium has traditionally been considered sterile. Nowadays, active studies are performed on the female upper genital tract microbiota. Bacteria and/or viruses colonizing the endometrium are known to alter its functional properties, including receptivity and embryo implantation. Uterine cavity inflammation caused by microorganisms leads to disrupted cytokine expression, which, in turn, is mandatory for the successful implantation of the embryo. The present study assessed the vaginal and endometrial microbiota composition and its relation to the levels of cytokines produced by the endometrium in reproductive-aged women complaining of secondary infertility of unknown origin. The multiplex real-time PCR assay was applied for vaginal and endometrial microbiota analysis. The quantitative measurement of endometrial α-defensin (DEFa1), transforming growth factor (TGFβ1), and basic fibroblast growth factor (bFGF2) was carried out using the ELISA (Cloud-Clone Corporation (Katy, TX, USA; manufactured in Wuhan, China). A reliable decline in endometrial TGFβ1 and bFGF2 and an increase in DEFa1 were demonstrated in women with idiopathic infertility when compared to fertile patients. However, TGFβ1, bFGF2, and DEFa1 expression correlated reliably only with the presence of Peptostreptococcus spp. and HPV in the uterine cavity. The obtained results highlight the importance of local immune biomarker determination in the assessment of certain bacteria and viruses’ significance as causative agents of infertility.

show abstract

Immunological uterine response to pig embryos before and during implantation

Cited by 10 publications

References 63 publications

In-depth proteome characterization of endometrium and extraembryonic membranes during implantation in pig

In-depth proteome characterization of endometrium and extraembryonic membranes during implantation in pig

Integrated Analysis of DNA Methylation and Gene Expression in Porcine Placental Development

Local Immune Biomarker Expression Depending on the Uterine Microbiota in Patients with Idiopathic Infertility

Contact Info

Product

Resources

About