Immunology of bovine heart valves. II. Reaction with connective tissue components.

Abstract

EnglandIn the previous paper we examined the evidence for a cross-reaction between heart valves and the Cpolysaccharide of Group A streptococci. In this paper we examine the reactions of sera raised against whole heart valves with the chemical fractions of the valves to see if the fractionation techniques separate the antigenic components. Also we examine the reaction of the sera with cryostat sections of human tissue to see in which tissues of the human body antigenic determinants shared with the valves are present. In this immunofluorescent study the reactions were compared with those given by antistreptococcal sera to see The CTC extracts isolated with either technique gave three to five lines with antiBHV sera, the number of lines being reduced to two with sera which had been absorbed with dried bovine serum (100 mg./ml.).The crude soluble collagen (CSC) fractions gave patterns very similar to those given by CTC extracts. As has been discussed in the previous paper, CSC-D gave a faint non-specific line with all sera. The solutions of this particular fraction for double diffusion purpose were made by dissolving it in 8 M urea and dialysing against buffered saline pH 7-2.The CTC extract and crude soluble collagen contained at least one antigen in common which gave a fused identity line.VSGP (valvular structural glycoprotein), isolated according to the original procedure, gave two or three lines with homologous sera, of which one or two persisted when sera absorbed with bovine serum were used. VSGP-D gave only one line with one of the lines given by VSGP. VSGP (but not VSGP-D) gave a weak non-specific line with all sera, probably owing to the collagen present.AntiBHV sera were absorbed with different fractions and the absorbed sera reacted with the fractions. Double absorption with CTC at concentration 10 mg./ml. removed all antibodies against any valvular fraction. Sera absorbed with CSC or CSC-D still gave one line when tested against CTC or VSGP fractions. VSGP absorbed sera gave single line with CTC and CSC and no linewith VSGP-D. VSGP-D absorbed sera were still able to give two or three on 27 April 2019 by guest. Protected by copyright.

Polyphenol-Stabilized Tubular Elastin Scaffolds for Tissue Engineered Vascular Grafts

“…1 Artificial blood vessels made of synthetic materials have been successful in large-diameter replacements, but they fail when used in small-diameter applications (<5-6 mm) because of poor patency and early graft occlusion. [2][3][4][5] In the United States, more than 1 million vascular procedures are performed each year involving smalldiameter vessels.…”

Induction of myosin cross-reactive antibody and cytolytic T cell responses in mice with Streptococcus pyogenes

“…Likewise, antibodies raised against bovine heart valve homogenates were not cross-reactive to GAS polysaccharide (Kasp-Grochowska et al 1972a). Upon histological staining of human heart and kidney tissue with this bovine heart antisera and anti-streptococcal sera they found that the bovine heart sera reacted to connective tissue and basement membrane while the anti-streptococcal sera reacted to cellular components of both myocytes and fibroblasts in the heart and the glomerular tufts of the kidney (Kasp-Grochowska et al 1972b). Demonstration of this cross-reactivity leads to another interesting facet of the autoimmune response; a bacterial carbohydrate is capable of eliciting antibodies that can cross-react with protein determinants.…”