Immunoluminometric assay for quantification of peroxiredoxin 4 in human serum

Schulte, Janin; Struck, Joachim; Bergmann, Andreas; Köhrle, Josef

doi:10.1016/j.cca.2010.05.016

Cited by 22 publications

(33 citation statements)

References 25 publications

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“…In earlier studies, specific antibodies have been prepared using an inactive denatured polypeptide or a synthetic polypeptide as the antigen (21,23,27). The use of the native form of a protein can occassionally prove more benificial for immunization.…”

Section: Antibody Raised Against Native Prx-4 Proteinmentioning

confidence: 99%

“…Thus, the quantification of Prx-4 may also be useful for the clinical examination of pathological conditions using serum samples. Although an attempt to examine the serum levels of Prx-4 has recently been reported for sepsis in humans, a complete quantification was not accomplished due to the unavailability of a standard sample and the use of an arbitrary unit which was unique to this and its use as a potential marker for hepatic disease RITSU ITO 1 , MOTOKO TAKAHASHI 2 , HIDEYUKI IHARA 1 , HIROKI TSUKAMOTO 1 , earlier study (23). In addition, it has not yet been fully clarified in which pathological conditions or in which types of disease this protein is implicated, although available evidence suggests that Prx-4 plays a role in ER stress and the oxidative folding of proteins in the ER (24,25).…”

Section: Introductionmentioning

confidence: 99%

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Measurement of peroxiredoxin-4 serum levels in rat tissue and its use as a potential marker for hepatic disease

Ito

Takahashi

Ihara

et al. 2012

Molecular Medicine Reports

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Abstract. Peroxiredoxin (Prx)-4, a secretable endoplasmic reticulum (ER)-resident isoform of the mammalian Prx family, functions as a thioredoxin-dependent peroxidase. It is acknowledged that Prx-4 plays a role in the detoxification of hydrogen peroxide, and potentially other peroxides, which may be generated during the oxidative folding of proteins and oxidative stress in the ER. The present study was undertaken in order to specifically quantify the tissue levels of Prx-4. To accomplish this, an enzyme-linked immunosorbent assay was developed using a specific polyclonal antibody produced by immunizing a rabbit with native recombinant rat Prx-4 protein. The assay was used to detect Prx-4 in the range of 0.1 and 10 ng/ml, and to investigate tissue distribution in rats. Using this immunoassay, we found that the serum levels of Prx-4 were substantially lower in asymptomatic Long-Evans Cinnamon rats, a rat model of Wilson's disease, compared to normal rats. In addition, the treatment of rat hepatoma cells with N-acetylcysteine led to a significant increase in the release of Prx-4 protein into the medium; thus, it appears likely that the secretion of Prx-4 is associated with the redox state within cells. These results suggest that serum Prx-4 has potential for use as a biomarker for hepatic oxidative stress.

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Section: Antibody Raised Against Native Prx-4 Proteinmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Measurement of peroxiredoxin-4 serum levels in rat tissue and its use as a potential marker for hepatic disease

Ito

Takahashi

Ihara

et al. 2012

Molecular Medicine Reports

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“…Under such conditions, damaged postsynaptic membranes could release PRX5 and induce further inflammatory reactions. Several studies have reported increased PRXs levels in carcinoma, sepsis, sarcoidosis, and stroke (Shichita et al, 2012;Schulte et al, 2010;Silva et al, 2007;Fujita et al, 2006). Autoantibodies against PRXs have been described in patients with rheumatoid arthritis, systemic lupus erythematosus, and systemic vasculitis (Karasawa et al, 2005).…”

Section: Discussionmentioning

confidence: 96%

Increased serum peroxiredoxin 5 levels in myasthenia gravis

Uzawa

Kawaguchi

Kanai

et al. 2015

Journal of Neuroimmunology

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“…The signal sequence is cleavable and allows the enzyme to be extracellularly released. In cultured cells, secretion of PRDX4 protein into the medium has been observed, and significant levels of PRDX4 were also detected in human and rat serum by immunological assay using specific antibodies [20,21].…”

Section: Structure and Molecular Organization Of Prdx4mentioning

confidence: 96%

“…Immunological assay using specific antibodies has enabled quantification of the plasma levels of PRDX4 [20,21]. However, the fate of PRDX4, either as ER resident or in secretion, appears to depend on the types of the cells, because no secretion has been observed in some types of cells [19].…”

Section: Extracellular Prdx4mentioning

confidence: 99%