Immunomagnetic Removal of Calla Positive Cells From Human Bone Marrow

Poynton, C. J.; Dicke, K. A.; Culbert, Steven J.; Frankel, LawrenceS; Jagannath, Sundar; Reading, C. L.

doi:10.1016/s0140-6736(83)92206-7

Cited by 41 publications

(9 citation statements)

References 5 publications

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“…Heat (13,14), light (15)(16)(17)(18)(19), or magnetism (20,21) have been used to activate drugs in particular tissues, and magnetism has been used to concentrate in tumors drugs held in microspheres (22). Only a subset of the cells in the region subjected to physical activation would be destroyed if the drug were delivered to them as a protein conjugate.…”

mentioning

confidence: 99%

Selective killing of T lymphocytes by phototoxic liposomes.

Yemul¹,

Berger²,

Estabrook³

et al. 1987

Proc. Natl. Acad. Sci. U.S.A.

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Two-fold specificity in drug delivery obtained through (i) the localized activation of drugs by physical means and (ii) the attachment of drugs to proteins that bind to target cells might be used for highly selective cancer chemotherapy or for immunosuppression. Toward this end, a monoclonal antibody against an antigen on the surface of T lymphocytes was covalently attached to liposomes containing a phototoxic drug, pyrene, bound to the lipid bilayer. When unfractionated peripheral blood lymphocytes, or B-and T-cell lines, were irradiated after treatment with these liposomes, T cells were killed while B cells were spared, demonstrating the validity of the approach in a simple in vitro assay.

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mentioning

confidence: 99%

Selective killing of T lymphocytes by phototoxic liposomes.

Yemul¹,

Berger²,

Estabrook³

et al. 1987

Proc. Natl. Acad. Sci. U.S.A.

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“…Overall, these are ‘bulk' sorting methods that allow isolation of large numbers of cells in a relatively short period of time. Among these techniques, the most extensively used are based on cell density properties that can be revealed by centrifugation; however, cell sedimentation (density-based) and filtration (size-based) methods have also been extensively employed [41,42]. In practice, density-gradient centrifugation techniques are mostly used for the isolation of different types of cells from PB and blood-containing samples, as well as to exclude dying cells from other tissue samples.…”

Section: Cell Purification Technologiesmentioning

confidence: 99%

“…Of note, these techniques are relatively simple methods which are generally employed for cell enrichment as a preparative tool for further cell purification steps [42]. This is due to the fact that filtration techniques are useful approaches for the removal of debris, dead cells and cell aggregates, particularly during the preparation of single cell suspensions from solid tissues.…”

Section: Cell Purification Technologiesmentioning

confidence: 99%

Cell Purification: A New Challenge for Biobanks

Almeida¹,

García‐Montero²,

Órfão³

2014

Pathobiology

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Performing ‘-omics' analyses on heterogeneous biological tissue samples, such as blood or bone marrow, can lead to biased or even erroneous results, particularly when the targeted cells and/or molecules are present at relatively low percentages/amounts. In such cases, whole sample analysis will most probably dilute and mask the features of the cell and/or molecules of interest, and this will negatively impact the results and their interpretation. Therefore, frequently it is critically important to have well-characterized and high-quality purified cell populations for the reliable detection of subtle variations in their specific features, such as gene expression profile, protein expression pattern and metabolic status. Biobanks are technological platforms which aim to provide researchers access to a large number of high-quality biological samples and their associated data, particularly to support high-quality scientific and clinical research projects, and such projects will benefit enormously by having access to high-quality purified cell populations or their biological components (e.g. DNA, RNA, proteins). Therefore, a clear opportunity exists for preparative cell sorting techniques in biobanks. Although multiple different cell purification approaches exist or are under development (e.g. cell purification techniques based on cell adherence, density and/or cell size properties, methods based on antibody binding as well as new lab-on-a-chip purification techniques), the choice for a specific technology depends on multiple variables, including cell recovery, purity and yield, among others. In addition, most cell purification approaches are not well suited for high-throughput (HT) purification of multiple cell populations coexisting in a sample. Here we review the most (currently) used cell sorting methods that may be applied for sample preparation in biobanks. For the different approaches, technical considerations about their advantages and limitations are highlighted, and the requirements to be met by a HT cell sorting technology to be used in biobanks are also discussed.

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“…When combined with the use of antibodies, physical activation permits the controlled destruction of a preselected subset of cells within the area of drug activation. In a related approach, target cells that have taken up metallic microspheres have been removed from bone marrow by magnets [33,42].…”

Section: Introductionmentioning

confidence: 99%

Phototoxic liposomes coupled to an antibody that alone cannot modulate its cell-surface antigen kill selected target cells

Yemul

Berger

Katz

et al. 1990

Cancer Immunol Immunother

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Molecules such as antibodies that bind to cell surfaces can be used to deliver cytotoxic drugs to selected cells. To be effective the drug must usually be taken into the cells by endocytosis. In this study a T-cell line (CCRF-CEM) was effectively killed by liposomes carrying a photosensitizer and bearing the antibody OKT4 (anti-CD4). The unconjugated antibody does not induce antigenic modulation in the target cells, an indication of the absence of endocytosis, and would therefore not normally have been selected as an agent for drug delivery. It cannot, however, be concluded with certainty that the conjugates act at the cell surface and several alternative explanations of their efficacy are offered.

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Immunomagnetic Removal of Calla Positive Cells From Human Bone Marrow

Cited by 41 publications

References 5 publications

Selective killing of T lymphocytes by phototoxic liposomes.

Selective killing of T lymphocytes by phototoxic liposomes.

Cell Purification: A New Challenge for Biobanks

Phototoxic liposomes coupled to an antibody that alone cannot modulate its cell-surface antigen kill selected target cells

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