Immunomagnetic Separation of Fat Depot-specific Sca1&lt;sup&gt;high&lt;/sup&gt; Adipose-derived Stem Cells (ASCs)

Barnes, Richard H.; Chun, Tae Hwa

doi:10.3791/53890

Cited by 2 publications

(2 citation statements)

References 17 publications

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“…After adding growth medium (DMEM containing 10% FBS, 20 mM glutamine, 100 units/ml penicillin, 100 units/ml streptomycin, and 250 ng/ml amphotericin B), the cell suspension was passed through a 100-m cell strainer again and centrifuged for 10 min at 1,500 rpm and seeded onto a culture plate. The cultured primary cells were applied to MACS separator (Miltenyi Biotec, Auburn, CA) to enrich for Sca1 high ASCs as previously reported (9,29).…”

Section: Tcf21 In Adipose Il6 and Ecm Remodeling Isolation Of Sca1 Himentioning

confidence: 99%

Transcription factor 21 (TCF21) promotes proinflammatory interleukin 6 expression and extracellular matrix remodeling in visceral adipose stem cells

Akama

Chun

2018

Journal of Biological Chemistry

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The visceral (VIS) and subcutaneous (SQ) fat pads are developmentally distinct white adipose tissue depots and contribute differently to inflammation and insulin resistance associated with obesity. The basic helix-loop-helix transcriptional regulator, transcription factor 21 (TCF21), is a marker gene for white adipose tissues and is abundantly expressed in VIS-derived adipose stem cells (ASCs), but not in SQ-derived ASCs. However, TCF21's role in regulating fat depot-specific gene expression and function is incompletely understood. Here, using siRNA-mediated knockdowns and lentiviral gene transfer of in mouse ASCs, we demonstrate that TCF21 is required for the VIS ASC-specific expression of interleukin 6 (IL6), a key cytokine that contributes to the proinflammatory nature of VIS depots. Concurrently, TCF21 promotes MMP-dependent collagen degradation and type IV collagen deposition through the regulation of the extracellular matrix (ECM) modifiers, matrix metalloproteinase (MMP) 2, MMP13, and tissue inhibitor of MMP1 (TIMP1), as well as collagen type IV α1 chain (COL4A1) in VIS ASCs. We also found that although IL6 mediates the expression of and in VIS ASCs, the TCF21-dependent expression of and is IL6-independent. These results suggest that TCF21 contributes to the proinflammatory environment in VIS fat depots and to active ECM remodeling of these depots by regulating IL6 expression and MMP-dependent ECM remodeling in a spatiotemporally coordinated manner.

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Section: Tcf21 In Adipose Il6 and Ecm Remodeling Isolation Of Sca1 Himentioning

confidence: 99%

Transcription factor 21 (TCF21) promotes proinflammatory interleukin 6 expression and extracellular matrix remodeling in visceral adipose stem cells

Akama

Chun

2018

Journal of Biological Chemistry

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“…Mouse adipose tissue progenitor cells were isolated and cultured as previously described [23]. Differentiation medium consisting of DMEM supplemented with 10% FBS, 1% penicillin/streptomycin, 0.5 m m isobutylmethylxanthine (IBMX) (Sigma), 1 μg·mL −1 insulin (Sigma) and 1 μ m dexamethasone (Sigma) was added to the cells 2 days after they reached confluence (day 0).…”

Section: Methodsmentioning

confidence: 99%

Periostin deficiency attenuates lipopolysaccharide‐ and obesity‐induced adipose tissue fibrosis

et al. 2021

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Periostin (POSTN) is a type of matricellular protein, but its functions in adipose fibrosis remain unclear. Here, we found that POSTN expression is significantly increased in mouse adipose tissue after treatment with lipopolysaccharide (LPS) or a high‐fat diet (HFD) and that adipose progenitor cells are the main source of POSTN. In our mouse model of fibrosis, POSTN deletion protected mice from adipose fibrosis, probably through reducing the accumulation of macrophages and promoting adipocyte differentiation of progenitor cells. Taken together, our study demonstrates that POSTN deficiency attenuates adipose tissue fibrosis and improves insulin resistance, providing new insights into the diagnosis and treatment of type II diabetes by targeting adipose tissue fibrosis.

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Immunomagnetic Separation of Fat Depot-specific Sca1<sup>high</sup> Adipose-derived Stem Cells (ASCs)

Cited by 2 publications

References 17 publications

Transcription factor 21 (TCF21) promotes proinflammatory interleukin 6 expression and extracellular matrix remodeling in visceral adipose stem cells

Transcription factor 21 (TCF21) promotes proinflammatory interleukin 6 expression and extracellular matrix remodeling in visceral adipose stem cells

Periostin deficiency attenuates lipopolysaccharide‐ and obesity‐induced adipose tissue fibrosis

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