Immunomic Identification of Malaria Antigens Associated With Protection in Mice

Siau, Anthony; Huang, Xiucai; Loh, Han Ping; Zhang, Neng; Meng, Wei; Sze, Siu Kwan; Rénia, Laurent; Preiser, Peter R.

doi:10.1074/mcp.ra118.000997

Cited by 2 publications

(2 citation statements)

References 101 publications

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“…Reverse Previous proteomics screening and analysis has generated a list of proteins associated with protection prior to me joining the lab as a PhD student [135]. I have included these works in Section 4.1 and they served as background information for the actual work that was done by me from section 4.2 onwards.…”

Section: Cdna Synthesis By Reverse Transcriptionmentioning

confidence: 99%

“…A list of 202 proteins that are potentially associated with protection was generated by a proteomics screen in our laboratory [135]. Using the scoring matrix defined in table 7, the 202 proteins potentially associated with protection were ranked, with proteins having a score of ≥8 being considered for subsequent analysis.…”

Section: Three Novel Proteins Of Unknown Function Were Shortlistedmentioning

confidence: 99%

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Exported proteins and their role in Plasmodium infection

Loh¹

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Section: Cdna Synthesis By Reverse Transcriptionmentioning

confidence: 99%

Section: Three Novel Proteins Of Unknown Function Were Shortlistedmentioning

confidence: 99%

Exported proteins and their role in Plasmodium infection

Loh¹

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Identification of the immunogenic membrane proteins, catalase, PgbA, and PgbB, as potential antigens against Helicobacter pylori

Li,

Zhang,

et al. 2023

Journal of Applied Microbiology

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Aims This study aims to investigate the specific membrane antigens that are targeted by antibodies raised against H. pylori. Methods and results Bovine milk antibodies were prepared using whole H. pylori, purified membrane proteins, or both. Enzyme-linked immunosorbent assay (ELISA) and sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) experiments revealed that these immunogens triggered anti-H. pylori antibody production in milk. The highest antibody titer was induced by the mixture of whole bacteria and purified membrane proteins. The antibodies induced by mixed immunogens significantly inhibited H. pylori growth in vitro and were used to identify catalase, plasminogen-binding protein A (PgbA), and PgbB via western blotting, immunoprecipitation and two-dimensional western blotting (2D-WB) followed by LC–MS/MS. The immunogenicity of PgbA and PgbB was verified in mice vaccinated with their B-cell epitope vaccines. Following prophylactic vaccination of C57BL/6 mice, each of the three antigens alone and their combination reduced the weight loss in mice, increased antibody titers, and relieved the inflammatory status of the gastric mucosa following H. pylori infection. Conclusions Catalase, PgbA, and PgbB could serve as valuable membrane antigens for the development of anti-H. pylori immunotherapies.

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Immunomic Identification of Malaria Antigens Associated With Protection in Mice

Cited by 2 publications

References 101 publications

Exported proteins and their role in Plasmodium infection

Exported proteins and their role in Plasmodium infection

Identification of the immunogenic membrane proteins, catalase, PgbA, and PgbB, as potential antigens against Helicobacter pylori

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