Immunomodulating activity of the polysaccharide TLH-3 from Tricholomalobayense in RAW264.7 macrophages

Zhang, Mingzhu; Tian, Xiaohe; Wang, Ya; Wang, Dandan; Wan, Li; Chen, Lei; Pan, Wenjun; Mehmood, Shomaila; Chen, Yan

doi:10.1016/j.ijbiomac.2017.10.165

Cited by 53 publications

(24 citation statements)

References 32 publications

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“…Moreover at higher dose of 200 μg/ml treatment, phagocytic activity was decreased by 85%. Similar activity was noticed in case of pure polysaccharide, TLH3 but at much higher dose of about 200 μg/ml polysaccharide treatment suggesting the superiority of crude fraction over the purified element on macrophage cells …”

Section: Resultssupporting

confidence: 77%

“…The similar phenomenon has been reported by other authors. [39] In previous publication by Zhang et al [18] the pure polysaccharide, TLH3 activated macrophages proliferation at 200 lg/ml implying that the crude form acted much better at lower concentration than pure one.…”

Section: Mlhwp Effect On Raw 2647 Macrophage Cells Proliferationmentioning

confidence: 93%

“…Similar activity was noticed in case of pure polysaccharide, TLH3 but at much higher dose of about 200 lg/ml polysaccharide treatment suggesting the superiority of crude fraction over the purified element on macrophage cells. [18] Influence of MLHWP on nitric oxide production from macrophages NO plays a diverse role in cardiovascular and immune system as a secondary messenger as well as an effector molecule. Herein, polysaccharide administration significantly enhanced NO production (28.4 lM) maximally at 50 lg/ml and approximatey twofold higher in comparison with the control set (13.3 lM; Figure 2c).…”

Section: Determination Of Phagocytic Activitymentioning

confidence: 99%

“…Among them, one of the purified polysaccharide (TLH 3) obtained from fruiting bodies of this mushroom was mainly evaluated for biological properties . This pure polysaccharide exhibited strong free radical scavenging potency, antiageing activity as well as immunomodulatory efficacy . Recently, our research group for the first time documented the phenolic profile and bioactivities of the methanolic extract obtained from wild M. lobayensis .…”

Section: Introductionmentioning

confidence: 99%

“…[15,16] This pure polysaccharide exhibited strong free radical scavenging potency, antiageing activity as well as immunomodulatory efficacy. [17,18] Recently, our research group for the first time documented the phenolic profile and bioactivities of the methanolic extract obtained from wild M. lobayensis. [19] However, there is no such research regarding the crude polysaccharide of this mushroom until now.…”

Section: Introductionmentioning

confidence: 99%

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Crude polysaccharide from a wild mushroom enhances immune response in murine macrophage cells by TLR/NF-κB pathway

Ghosh

Khatua

Acharya

2019

Journal of Pharmacy and Pharmacology

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Objective Mushroom crude polysaccharides offer a complete package of various medicinal activities. In this context, the present study aimed to unveil structural and biomedical properties of crude polysaccharide (MLHWP) obtained from an edible wild mushroom Macrocybe lobayensis (R. Heim) Pegler & Lodge. Method Chemical characterization was accomplished with the help of spectrophotometry, Fourier‐transform infrared spectroscopy, HPTLC and GC‐MS. Immunomodulatory activity of the crude polysaccharide and its signalling mechanism was assessed using RAW 264.7 cells. Furthermore, antioxidant activity was analysed based on radical scavenging, metal ion chelating and reducing effect. Key findings Compositional study revealed that MLHWP possessed triple helical structure and its backbone consisted of β‐linked glucan along with xylose, rhamnose, mannose and galactose. Investigation on bioactive potency revealed that MLHWP augmented macrophage activity in terms of viability, phagocytosis, NO and ROS generation. Gene expression studies indicated that MLHWP signalled through TLR and modulated expression of immunomodulation‐related genes including NF‐κB, COX‐2, IFN‐γ, TNF‐α, iNOS and Iκ‐βα. Besides, MLHWP displayed noticeable antioxidant potential as reflected in all investigating assays. Conclusions Overall, the results portrayed possibility of MLHWP as pharmaceutical agent with multidimensional application.

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Section: Resultssupporting

confidence: 77%

Section: Mlhwp Effect On Raw 2647 Macrophage Cells Proliferationmentioning

confidence: 93%

Section: Determination Of Phagocytic Activitymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Crude polysaccharide from a wild mushroom enhances immune response in murine macrophage cells by TLR/NF-κB pathway

Ghosh

Khatua

Acharya

2019

Journal of Pharmacy and Pharmacology

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Garlic‐Derived Organosulfur Compounds Regulate Metabolic and Immune Pathways in Macrophages and Attenuate Intestinal Inflammation in Mice

Zhu

Myhill

Andersen-Civil

et al. 2022

Molecular Nutrition Food Res

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Scope: Garlic is a source of bioactive phytonutrients that may have anti‐inflammatory or immunomodulatory properties. The mechanism(s) underlying the bioactivity of these compounds and their ability to regulate responses to enteric infections remains unclear. Methods and Results: This study investigates if a garlic‐derived preparation (PTSO‐PTS) containing two organosulfur metabolites, propyl‐propane thiosulfonate (PTSO), and propyl‐propane thiosulfinate (PTS), regulate inflammatory responses in murine macrophages and intestinal epithelial cells (IEC) in vitro, as well as in a model of enteric parasite‐induced inflammation. PTSO‐PTS decreases lipopolysaccharide‐induced secretion of TNFα, IL‐6, and IL‐27 in macrophages. RNA‐sequencing demonstrates that PTSO‐PTS strongly suppresses pathways related to immune and inflammatory signaling. PTSO‐PTS induces the expression of a number of genes involved in antioxidant responses in IEC during exposure to antigens from the parasite Trichuris muris. In vivo, PTSO‐PTS does not affect T. muris establishment or intestinal T‐cell responses but significantly alters cecal transcriptomic responses. Notably, a reduction in T. muris‐induced expression of Tnf, Saa2, and Nos2 is observed. Conclusion: Garlic‐derived organosulfur compounds exert anti‐inflammatory effects in macrophages and IEC, and regulate gene expression during intestinal infection. These compounds and related organic molecules may thus hold potential as functional food components to improve gut health in humans and animals.

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Structural elucidation and immunomodulatory activity of a neutral polysaccharide from the Kushui Rose (Rosa setate x Rosa rugosa) waste

Feng²,

Song

et al. 2020

Carbohydrate Polymers

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Immunomodulating activity of the polysaccharide TLH-3 from Tricholomalobayense in RAW264.7 macrophages

Cited by 53 publications

References 32 publications

Crude polysaccharide from a wild mushroom enhances immune response in murine macrophage cells by TLR/NF-κB pathway

Crude polysaccharide from a wild mushroom enhances immune response in murine macrophage cells by TLR/NF-κB pathway

Garlic‐Derived Organosulfur Compounds Regulate Metabolic and Immune Pathways in Macrophages and Attenuate Intestinal Inflammation in Mice

Structural elucidation and immunomodulatory activity of a neutral polysaccharide from the Kushui Rose (Rosa setate x Rosa rugosa) waste

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