Immunomodulating and antiviral activities of Uncaria tomentosa on human monocytes infected with Dengue Virus-2

Reis, Sonia Regina I.N.; Valente, Lígia M. M.; Sampaio, André Luiz Franco; Siani, Antonio C.; Gandini, Mariana; Azeredo, Elzinandes Leal de; d’Avila, Luiz Antonio; Mazzei, José Luiz; Henriques, Maria das Graças; Kubelka, Claire Fernandes

doi:10.1016/j.intimp.2007.11.010

Cited by 85 publications

(92 citation statements)

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“…Carbohydrate-binding agents originated from plant lectins can affect the binding of the virus to cell and subsequently inhibit viral replication (Talarico et al 2007, Alen et al 2009). Inhibitory activities by both extract and alkaloidal fraction of Uncaria tomentosa on infection rates and cytokine levels in treated monocytes-DENV infected have been reported (Reis et al 2008). It seems that the zosteric acid derived from Zostera marina interferes with binding to the receptor required for DENV entry into the host cell (Rees et al 2008).…”

Section: Resultsmentioning

confidence: 99%

Virucidal activity of Colombian Lippia essential oils on dengue virus replication in vitro

Ocazionez

Meneses

Torres

et al. 2010

Mem. Inst. Oswaldo Cruz

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The inhibitory effect of Lippia alba and Lippia citriodora essential oils on dengue virus serotypes replication in vitro was investigated. The cytotoxicity (CC 50 ) was evaluated by the MTT assay and the mode of viral inhibitory effect was investigated with a plaque reduction assay. The virus was treated with the essential oil for 2 h at 37 o C before cell adsorption and experiments were conducted to evaluate inhibition of untreated-virus replication in the presence of oil. Antiviral activity was defined as the concentration of essential oil that caused 50% reduction of the virus plaque number (IC 50 ). L. alba oil resulted in less cytotoxicity than L. citriodora oil (CC 50 : 139.5 vs. 57.6 µg/mL). Virus plaque reduction for all four dengue serotypes was observed by treatment of the virus before adsorption on cell. The IC 50 values for L. alba oil were between 0. 4-32.6 µg/mL and between 1.9-33.7 µg/mL for L. citriodora oil. No viral inhibitory effect was observed by addition of the essential oil after virus adsorption. The inhibitory effect of the essential oil seems to cause direct virus inactivation before adsorption on host cell.Key words: essential oil -antiviral -virucidal activity -dengue virus -Lippia sp.Dengue is an acute illness characterised by the hemorrhagic fever caused by dengue virus (DENV) that is transmitted to humans by Aedes mosquitoes. DENV infection results in a spectrum of disease ranging from a mild febrile illness or dengue fever to a severe disease or dengue hemorrhagic fever/dengue shock syndrome. Among the human arboviral diseases, dengue is responsible for causing most illness and death. It has been estimated that 50-100 million dengue infections occur each year in tropical urban areas around the world with 20,000-25,000 deaths (Gubler 2006).DENV is a member of the Flaviviridae family and it exists as four related serotypes (DENV-1 2, 3 and 4). The virus possesses an icosahedral nucleocapsid core surrounded by a host-derived lipid membrane (envelope), in which the envelope (E) protein and membrane (M) protein are embedded. The Flavivirus enters into the host cell by receptor-mediated endocytosis into coated vesicles, a process in which the E protein plays the major role. The E protein mediates interactions within the virus and the components of the host cell membrane involved in virus adsorption and the E protein interacts with the membrane cell during fusion (Lindenbach & Rice 2007). Compounds that interact with sites on the E protein that binds to structures on the cellular membrane affect the adsorption and fusion process. That interferes There is no antiviral drug for treatment for any of the Flavivirus and an effective vaccine for human use is not yet available to prevent dengue. Consequently, the development of antiviral drugs licensed for treatment of patients remains an urgent need to prevent dengue fatalities. Compounds obtained from traditional medicinal plants and herbs species have been reported to have antiviral activity and a wide variety of active phytochemicals hav...

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Section: Resultsmentioning

confidence: 99%

Virucidal activity of Colombian Lippia essential oils on dengue virus replication in vitro

Ocazionez

Meneses

Torres

et al. 2010

Mem. Inst. Oswaldo Cruz

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“…Medicine-Uncaria interactions have not been objectively evaluated in published peer-reviewed studies (Rotblatt et al, 2002;Pilarski et al, 2006;Reis et al, 2008). Thus, it is important to develop models that can describe the possible in vitro and in vivo drug interactions.…”

Section: Discussionmentioning

confidence: 99%

“…Uncaria tomentosa, also known as unha de gato ('Cats claw' in English), has become widely known as an useful plant in ethnomedicine. This plant is traditionally used to treat arthritis and rheumatism, ulcers and other disorders of the gastrointestinal tract, asthma, gonorrea, dengue, dysentery and tumors (Rotblatt et al, 2002;Pilarski et al, 2006;Reis et al, 2008). No side effects were reported in the few human studies, and no adverse effects were found in rodents (Rotblatt et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

“…No side effects were reported in the few human studies, and no adverse effects were found in rodents (Rotblatt et al, 2002). Uncaria-drugs interactions have not been well reported, but one in vitro study revealed Cats claw inhibition activity on the 3A4 isozime of cytochrome P450 (Reis et al, 2008). There are some studies about the effect of natural products on the labeling of blood constituents using technetium-99m (Tc-99m) (Lima et al, 2002;Oliveira et al, 1997;Braga et al, 2000;Oliveira et al, 2003;Dantas et al, 2005;Oliveira-Fernandes et al, 2005;Abreu et al, 2006;Neves et al, 2007;Rebello et al, 2007;Sinzinger et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

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Uncaria tomentosa extract: evaluation of effects on the in vitro and in vivo labeling of blood constituents with technetium-99m

Moreno

Carvalho

Nascimento

et al. 2008

Braz. arch. biol. technol.

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“…The crude aqueous extract of seeds of Guettarda angelica (Rubiaceae) showed in vitro antiviral activity against bovine (BoHV-1), swine (SuHV-1) herpes viruses type 1 (Barros et al, 2010) and avian viruses (Barros et al, 2013). It is reported (Reis et al, 2008) that the bark of the liana Uncaria tomentosa which is used traditionally (Williams, 2001) shows, in particular the alkaloid fraction, activity against the dengue virus type 2. Alkaloids are found to serve as a potential candidate to stop viral replication as in the case of dengue ns3 protease (Qamar et al 2014).…”

Section: Introductionmentioning

confidence: 99%

Antiviral activity of leaf-bud gum-resin of <i>Tarenna asiatica</i>

Ramabharathi¹,

Saigopal

Rajitha

2014

Bangladesh J Pharmacol

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The leaf-bud exudate of Tarenna asiatica (Rubiaceae: Ixoroideae, Pavetteae) is investigated for its biological activity. The crude benzene extract and corymbosin (pure compound isolated) were screened for antiviral activity by using ELISA and PCR methods against animal (blue tongue and chikungunya) and plant (papaya ring spot, sesbania mosaic and common bean mosaic) viruses. Both corymbosin and benzene extract showed significant antiviral activity though corymbosin was found relatively more potent against the animal and plant viruses tested. This is the first report of antiviral activity for the gumresin of T. asiatica, so also for the compound corymbosin, against the plant viruses. Article Info

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Immunomodulating and antiviral activities of Uncaria tomentosa on human monocytes infected with Dengue Virus-2

Cited by 85 publications

References 54 publications

Virucidal activity of Colombian Lippia essential oils on dengue virus replication in vitro

Virucidal activity of Colombian Lippia essential oils on dengue virus replication in vitro

Uncaria tomentosa extract: evaluation of effects on the in vitro and in vivo labeling of blood constituents with technetium-99m

Antiviral activity of leaf-bud gum-resin of <i>Tarenna asiatica</i>

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