Immunomodulating effects of casein-derived peptides QEPVL and QEPV on lymphocytes in vitro and in vivo

Zhou, Jian; Ma, Lu; Hai-Hong, Xu; Gao, Yang; Yingkai, Jin; Lei, Z. H.; Li, David Xi An; Zhan, Dongsheng; Zhang, Shaohui

doi:10.1039/c3fo60657k

Cited by 48 publications

(29 citation statements)

References 27 publications

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“…In an intestinal immune system, moderate pro‐inflammatory cytokines could play a beneficial role in host as a defence against intracellular pathogens, while the overproduction might result in pathological conditions and immune function decline (Barilli et al ., ; Jiehui et al ., ); therefore, the introduction of anti‐inflammatory cytokines is essential for maintaining a homoeostasis. The results in Fig.…”

Section: Resultsmentioning

confidence: 99%

Enzymatic preparation of immunomodulatory hydrolysates from defatted wheat germ (Triticum Vulgare) globulin

Zhang

Sun

et al. 2016

Int J of Food Sci Tech

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Summary Defatted wheat germ globulin (DWGG) was hydrolysed using the enzymes alcalase, neutrase, papain, pepsin or trypsin to improve the immunomodulatory effects of the protein. The degree of hydrolysis (DH), molecular weight distribution, hydrophobicity, secondary structures and immunomodulatory activities were determined. The DH of hydrolysate using alcalase or neutrase was higher (P < 0.05) than that of hydrolysate prepared by other proteases. Alcalase hydrolysate (AH) had the strongest (P < 0.05) immunomodulatory activity with respect to lymphocyte proliferation, phagocytosis of neutral red and secretion of pro‐inflammatory cytokines. The molecular weight of AH ranged between 300 and 1450 Da and was composed of α‐helix and β‐turns. AH also showed the highest hydrophobic value (P < 0.05). The DH and hydrophobicity appear to be related to immunomodulatory activities. The hydrolysate prepared by the alcalase treatment on DWGG may be used as an immune supplement by the food industry.

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Section: Resultsmentioning

confidence: 99%

Enzymatic preparation of immunomodulatory hydrolysates from defatted wheat germ (Triticum Vulgare) globulin

Zhang

Sun

et al. 2016

Int J of Food Sci Tech

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“…In another study, both these peptides were also shown to attenuate atherosclerosis development in apolipoprotein in E‐deficient mice (Nakamura et al, ). Another milk casein‐derived peptide, QEPV (Gln‐Glu‐Pro‐Val), was shown to inhibit the release of nitric oxide (NO) while increased the production of anti‐inflammatory mediators IL‐4 and IL‐10 (Jiehui et al, ). High hydrostatic pressure treated whey protein hydrolysates have also been shown to have anti‐inflammatory effects on intestinal epithelial and respiratory cells (Piccolomini, Iskandar, Lands & Kubow, ), although the peptides responsible for the activity has not been identified yet from the crude hydrolysate.…”

Section: Food‐derived Anti‐inflammatory Peptidesmentioning

confidence: 99%

Structural-features of food-derived bioactive peptides with anti-inflammatory activity: A brief review

2018

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Chronic inflammatory conditions such as obesity, type-2 diabetes, and cardiovascular diseases are the leading causes of mortality worldwide. Hence, much research interest in bioactive peptides has been stimulated due to lack of potent pharmacological interventions. Although many such peptides have been identified from food proteins, insufficient information is available on their structurefunction relationship. Presence of hydrophobic and positively charged amino acids is a common occurrence for the peptides with anti-inflammatory properties. However, inconsistent findings have also been reported. Most of the food-derived peptides exhibited their anti-inflammatory activities primarily by inhibiting signaling components of either NF-jB or MAPK pathway, which are the two major pathways involved in chronic inflammation following uncontrolled signal activation. This review highlighted the structural requirements of the peptides to exhibit antiinflammatory activity based on the current knowledge about food-derived anti-inflammatory peptides and their underlying molecular mechanisms of action. Practical applicationsWhile research in the food-derived bioactive peptide is gaining momentum, but the ability to translate these new findings into the commercial product such as nutraceuticals and functional foods, remains delayed. The most prominent reasons for this delay are the lack of detailed research on, (i) the structure-function relationship of the peptide and the underlying molecular mechanisms of these bioactive peptides, and (ii) the interaction of these peptides with different cellular elements in the disease pathophysiology. This review gives an insight into the structure-activity relationship of bioactive peptides involved in anti-inflammatory responses. The information provided here would be highly beneficial to describe the possible anti-inflammatory activity of any newly identified peptides from different food sources. K E Y W O R D Santi-inflammation, bioactive peptides, food proteins, inflammatory responses, structure-function relationships

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“…Amongst them, peptides with immunomodulatory ability had been focused on by more and more researchers. Casein-derived peptides QEPVL and QEPV could significantly increase mice lymphocyte proliferation rate and cAMP levels in vitro and also inhibited LPS-induced inflammation by regulating nitric oxide release and the production of the many cytokines in vivo (Zhou et al, 2014). Novel peptides isolated and characterized from the chicken bursa of Fabricius promoted the development of mice B cell and enhanced the production of specific Avian Influenza Virus antibody and cytokines, T-cell immunophenotyping at reachable concentrations (Liu et al, 2013).…”

Section: Introductionmentioning

confidence: 98%

Immunomodulatory activity of small molecular (≤3 kDa)Coixglutelin enzymatic hydrolysate

et al. 2016

CyTA - Journal of Food

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2017)Immunomodulatory activity of small molecular (≤3 kDa) Coix glutelin enzymatic hydrolysate, CyTA -Journal of Food, 15:1, 41-48, ABSTRACT In this study, small molecular (≤3 kDa) Coix glutelin hydrolysate (CGH) was obtained by the method of enzymatic hydrolysis and then immunomodulatory activity was evaluated in vitro and in vivo. Stimulation index of mice splenocytes was increased in a dose-dependent manner after treated with 3.125-25 μg/mL CGH. Acid phosphatase in mice peritoneal macrophages was significantly improved after stimulated with CGH at some concentration. The CGH stimulated the secretion of NO from Raw264.7 in a concentration-dependent manner and also significantly inhibited lipopolysaccharideinduced cell excessive activation at some extent. After successive administration of CGH for 21 days, the spleen index of ICR was significantly increased to 3.73 mg/g for the group administrated 800 mg/kg CGH. Malondialdehyde (MDA) assay displayed that 200 mg/kg CGH increased mice serum MDA concentration, while the variation was obsolete in the other CGH groups. All results demonstrated that CGH took essential effects on immunomodulatory activities.Actividad inmunomoduladora de hidrolisato glutelina encimático de moléculas pequeñas de Coix (≤ 3 kDa) RESUMEN En este estudio, se obtuvo hidrolisato glutelina de Coix (CGH) de molécula pequeña (≤ 3 kDa) mediante el método de hidrólisis enzimática y después se evaluó la actividad sinmunomoduladora in vitro y in vivo. El índice de estimulación (SI) de los esplenocitos de ratón aumentó de forma dependiente a la dosis después ser tratados con 3,125-25 μg/mL de CGH. La fosfatasa ácida (ACP) en los macrófagos peritoneales de ratón mejoró significativamente después de que se estimulara con CGH en algunas concentraciones. El CGH estimuló la secreción de NO a partir de Raw264.7 de forma dependiente de la concentración e inhibió de forma significativa la activación excesiva de células inducidas con LPS en cierta medida. Después de una administración sucesiva de CGH durante 21 días, el índice esplénico de ICR aumentó significativamente a 3,73 mg/g para el grupo al cual se había administrado 800 mg/kg de CGH. El ensayo de malondialdehído (MDA) mostró que los 200 mg/kg de CGH hicieron aumentar la concentración de MDA del suero de ratón, mientras que la variación fue obsoleta en los otros grupos de CGH. Todos los resultados demostraron que CGH obtuvo efectos esenciales en las actividades inmunomoduladoras. ARTICLE HISTORY

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Immunomodulating effects of casein-derived peptides QEPVL and QEPV on lymphocytes in vitro and in vivo

Cited by 48 publications

References 27 publications

Enzymatic preparation of immunomodulatory hydrolysates from defatted wheat germ (Triticum Vulgare) globulin

Enzymatic preparation of immunomodulatory hydrolysates from defatted wheat germ (Triticum Vulgare) globulin

Structural-features of food-derived bioactive peptides with anti-inflammatory activity: A brief review

Immunomodulatory activity of small molecular (≤3 kDa)Coixglutelin enzymatic hydrolysate

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