Immunomodulating Effects of Vilon and Its Analogue in the Culture of Human and Animal Thymus Cells

Sevostianova, N. N.; Linkova, N. S.; Polyakova, V. O.; Chervyakova, N. A.; Kostylev, A. V.; Durnova, A. O.; Kvetnoy, I. M.; Abdulragimov, R. I.; Khavinson, V. Kh.

doi:10.1007/s10517-013-2000-0

Cited by 10 publications

(5 citation statements)

References 9 publications

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“…Also, it has been observed that the KE peptide is capable of regulating gene expression of Interleukin-2 in blood lymphocytes, indicating its immunomodulating activity (35). The KE peptide also regulates expression of СD4, CD5 and CD8 glycoproteins in thymic cell culture and induces immune cell differentiation (36). In addition, the KE peptide was found to be capable to activate heterochromatin in the cell nuclei in senile patients and facilitate the “release" of genes suppressed as a result of heterochromatinization of chromosome euchromatin areas (14).…”

Section: Resultsmentioning

confidence: 99%

Systematic search for structural motifs of peptide binding to double-stranded DNA

Колчина

Khavinson

Linkova

et al. 2019

Nucleic Acids Research

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A large variety of short biologically active peptides possesses antioxidant, antibacterial, antitumour, anti-ageing and anti-inflammatory activity, involved in the regulation of neuro-immuno-endocrine system functions, cell apoptosis, proliferation and differentiation. Therefore, the mechanisms of their biological activity are attracting increasing attention not only in modern molecular biology, biochemistry and biophysics, but also in pharmacology and medicine. In this work, we systematically analysed the ability of dipeptides (all possible combinations of the 20 standard amino acids) to bind all possible combinations of tetra-nucleotides in the central part of dsDNA in the classic B-form using molecular docking and molecular dynamics. The vast majority of the dipeptides were found to be unable to bind dsDNA. However, we were able to identify 57 low-energy dipeptide complexes with peptide-dsDNA possessing high selectivity for DNA binding. The analysis of the dsDNA complexes with dipeptides with free and blocked N- and C-terminus showed that selective peptide binding to dsDNA can increase dramatically with the peptide length.

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Section: Resultsmentioning

confidence: 99%

Systematic search for structural motifs of peptide binding to double-stranded DNA

Колчина

Khavinson

Linkova

et al. 2019

Nucleic Acids Research

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“…This limits the glycolysis, biosynthesis, activation, and proliferation of T cells with aging [10,17]. Thus, the previously identified immunomodulatory and geroprotective effect of KE peptide on immune cells [2,4,5] can be realized via an increase in the functional activity of mitochondria.…”

Section: Area Of Mitochondrial Mitotracker Red Staining and The Expre...mentioning

confidence: 99%

“…The dipeptide vilon stimulates the cellular immunity and nonspecific resistance of the organism and also has a stimulating effect on macrophages and neutrophils [1,2]. The mechanism of action of KE peptide is associated with its activating effect on T cells, which contributes to the recognition of the complex of the peptide epitope with the molecule of the major histocompatibility complex located on the macrophage surface.…”

Section: Ribosome Functioning With Agingmentioning

confidence: 99%

Influence of AEDG and KE Peptides on Mitochondrial Staining and the Expression of Ribosomal Protein L7A with Aging of the Human Pineal Gland and Thymus Cell In Vitro

et al. 2021

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New molecular targets for the geroprotective activity of AEDG (epitalon) and KE (vilon) peptides were verified via confocal laser scanning microscopy. It was shown that the aging of pineal and thymic cells in vitro led to a decrease in the staining of MitoTracker Red mitochondria and that there is a compensatory increase in the synthesis of L7A ribosomal protein. AEDG peptide increased the area of MitoTracker Red mitochondrial staining by 1.5 times and decreased by 22% the expression of ribosomal protein L7A in cultures of human pineal-gland cells with aging. KE peptide increased the area of MitoTracker Red mitochondrial staining by 1.5 times and decreased by 15% the expression of ribosomal protein L7A in cultures of human thymic cells with aging. The area of MitoTracker Red mitochondrial staining decreased and the compensatory expression of L7A ribosomal protein increased with aging of the pineal gland and thymic cells. Presumably, AEDG and KE peptides have a tissue-specific effect that normalizes the functions of mitochondria and ribosomes of pinealocytes and thymocytes.

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“…The tetra peptide Epitalon was obtained from the peptide complex Epitalamin, derived from a bovine pineal gland, and it is involved mostly in the neuroendocrine system [ 32 , 33 , 34 ]. The tripeptide Chonluten, a synthetic bronchial bio-regulator, involves the respiratory system as the principal biological targets [ 35 , 36 ]. The synthetic dipeptide Vilon is implicated in regeneration of eye retinal cells and brain neurons and promotes cell proliferation and wound healing [ 37 , 38 , 39 ].…”

Section: Introductionmentioning

confidence: 99%

Peptides Regulating Proliferative Activity and Inflammatory Pathways in the Monocyte/Macrophage THP-1 Cell Line

Avolio

Martinotti

Хавинсон

et al. 2022

IJMS

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This study evaluates the effects of five different peptides, the Epitalon® tetrapeptide, the Vilon® dipeptide, the Thymogen® dipeptide, the Thymalin® peptide complex, and the Chonluten® tripeptide, as regulators of inflammatory and proliferative processes in the human monocytic THP-1, which is a human leukemia monocytic cell line capable of differentiating into macrophages by PMA in vitro. These peptides (Khavinson Peptides®), characterized by Prof. Khavinson from 1973 onwards, were initially isolated from animal tissues and found to be organ specific. We tested the capacity of the five peptides to influence cell cultures in vitro by incubating THP-1 cells with peptides at certain concentrations known for being effective on recipient cells in culture. We found that all five peptides can modulate key proliferative patterns, increasing tyrosine phosphorylation of mitogen-activated cytoplasmic kinases. In addition, the Chonluten tripeptide, derived from bronchial epithelial cells, inhibited in vitro tumor necrosis factor (TNF) production of monocytes exposed to pro-inflammatory bacterial lipopolysaccharide (LPS). The low TNF release by monocytes is linked to a documented mechanism of TNF tolerance, promoting attenuation of inflammatory action. Therefore, all peptides inhibited the expression of TNF and pro-inflammatory IL-6 cytokine stimulated by LPS on terminally differentiated THP-1 cells. Lastly, by incubating the THP1 cells, treated with the peptides, on a layer of activated endothelial cells (HUVECs activated by LPS), we observed a reduction in cell adhesion, a typical pro-inflammatory mechanism. Overall, the results suggest that the Khavinson Peptides® cooperate as natural inducers of TNF tolerance in monocyte, and act on macrophages as anti-inflammatory molecules during inflammatory and microbial-mediated activity.

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Immunomodulating Effects of Vilon and Its Analogue in the Culture of Human and Animal Thymus Cells

Cited by 10 publications

References 9 publications

Systematic search for structural motifs of peptide binding to double-stranded DNA

Systematic search for structural motifs of peptide binding to double-stranded DNA

Influence of AEDG and KE Peptides on Mitochondrial Staining and the Expression of Ribosomal Protein L7A with Aging of the Human Pineal Gland and Thymus Cell In Vitro

Peptides Regulating Proliferative Activity and Inflammatory Pathways in the Monocyte/Macrophage THP-1 Cell Line

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