Immunomodulating polyphenols from Sideritis scardica

Moussavi, Nastaran; Azizullah, Hasina; Malterud, Karl Egil; Inngjerdingen, Kari Tvete; Wangensteen, Helle

doi:10.1016/j.jff.2022.105197

Cited by 8 publications

(8 citation statements)

References 50 publications

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“…Depending on the region of origin, in Bulgaria, infusions from this herb are known as “Mursalski tea”, “Pirinski tea” or “Alibotushki tea”. In the Republic of North Macedonia, it is commonly named “Sharplaninsi chaj”, whereas in Greece, it can be called “Greek Mountain tea” or “Greek Olympus Tea” [ 5 , 6 , 7 ]. Sideritis raeseri is another popular herb variety endemic to the Balkans and the Iberian Peninsula, also cultivated in Greece [ 8 , 9 ].…”

Section: Introductionmentioning

confidence: 99%

Comparative Study on Assisted Solvent Extraction Techniques for the Extraction of Biologically Active Compounds from Sideritis raeseri and Sideritis scardica

et al. 2023

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The plants in the Sideritis genus are postulated to exhibit several important medicinal properties due to their unique chemical composition. To isolate the targeted phytochemical compounds, the selection of a suitable extraction method is of primary importance. In this work, a comparative study on the phytochemical profiles of various Sideritis raeseri and Sideritis scardica extracts has been carried out. An untargeted metabolomics approach based on ultra-high performance liquid chromatography coupled with high-resolution mass spectrometry was applied to investigate the metabolic differences between extracts obtained by conventional extraction and extractions assisted by microwaves, ultrasounds and high pressure. Additionally, the influence of extraction solvents on HPLC antioxidant profiles obtained following the derivatization of analytes with ABTS reagent was evaluated. A total of 102 metabolites have been putatively identified. The major secondary metabolites groups were classified as flavonoids, terpenoids, phenylethanoid glycosides and phenolic acids. The main antioxidants in the extracts were isoscutellarein and hypolaetin derivatives as well as verbascoside and chlorogenic acid. The results showed that 70% ethanol was the most effective extractant for different classes of phytochemicals including antioxidants. In addition, extraction supported with microwaves, ultrasounds or high pressure improved the overall recovery of metabolites by about 3 times compared to the conventional extraction method.

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Section: Introductionmentioning

confidence: 99%

Comparative Study on Assisted Solvent Extraction Techniques for the Extraction of Biologically Active Compounds from Sideritis raeseri and Sideritis scardica

et al. 2023

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“…Specifically, in mice, SS extracts have been shown to decrease soluble Aβ 1-42 and Aβ aggregation by enhancing the phagocytic microglia response of Aβ and inducing the expression of a-secretase ADAM10, which cleaves Aβ [36]. Here, we show that four SS extracts (SSDM, SSM, SSW1, and SSDE) can also reduce the cytotoxic activity of Aβ [25][26][27][28][29][30][31][32][33][34][35] , with SSW1 showing this potential in differentiated cells as well. In addition, for the first time, we present neuroprotectivity in SS extracts generated by using less-polar solvents, such as dichloromethane.…”

Section: Discussionmentioning

confidence: 68%

“…Extracts derived from SS have high contents of flavonoids and phenolics, substances with many pharmacological effects, such as antioxidant, anticancer, and antiviral properties [27][28][29]. Furthermore, methanolic and natural deep eutectic extracts of SS have demonstrated antiaging potential as well; ethanolic extracts showed remarkable anti-inflammatory, antimicrobial, and antioxidant capacities, while an SS dichloromethane extract exhibited anti-inflammatory activity [30][31][32][33]. SS hydroalcoholic extracts inhibit the reuptake of serotonin, noradrenaline, and dopamine.…”

Section: Introductionmentioning

confidence: 99%

Sideritis scardica Extracts Demonstrate Neuroprotective Activity against Aβ25–35 Toxicity

Ververis

Ioannou

Kyriakou

et al. 2023

Plants

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Alzheimer’s disease (AD) is the most prevalent neurodegenerative condition, primarily affecting seniors. Despite the significant time and money spent over the past few decades, no therapy has been developed yet. In recent years, the research has focused on ameliorating the cytotoxic amyloid beta (Aβ) peptide aggregates and the increased elevated oxidative stress, two interconnected main AD hallmarks. Medicinal plants constitute a large pool for identifying bioactive compounds or mixtures with a therapeutic effect. Sideritis scardica (SS) has been previously characterized as neuroprotective toward AD. We investigated this ability of SS by generating eight distinct solvent fractions, which were chemically characterized and assessed for their antioxidant and neuroprotective potential. The majority of the fractions were rich in phenolics and flavonoids, and all except one showed significant antioxidant activity. Additionally, four SS extracts partly rescued the viability in Aβ25–35-treated SH-SY5Y human neuroblastoma cells, with the initial aqueous extract being the most potent and demonstrating similar activity in retinoic-acid-differentiated cells as well. These extracts were rich in neuroprotective substances, such as apigenin, myricetin-3-galactoside, and ellagic acid. Our findings indicate that specific SS mixtures can benefit the pharmaceutical industry to develop herbal drugs and functional food products that may alleviate AD.

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“…The contribution of phenylethanoid glycosides to total phenolic content was around 50% for all samples, except for S. lanata where it accounted only for around 7% [ 6 ]. Eleven acetylated glycosides of isoscutellarein, hypolaetin, methylhypolaetin and methylisoscutellarein were previously isolated from 80% EtOH extract [ 30 ]. The differences between previous studies and our results can be ascribed to the different extraction methods and solvents.…”

Section: Resultsmentioning

confidence: 99%

A Comprehensive Phytochemical Analysis of Sideritis scardica Infusion Using Orbitrap UHPLC-HRMS

Zheleva-Dimitrova,

Voynikov,

Gevrenova

et al. 2023

Molecules

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Sideritis scardica Griseb, also known as “mountain tea” and “Olympus tea” (Lamiaceae family) is an endemic plant from the mountainous regions of the Balkan Peninsula. In this study, we focused on an in-depth phytochemical analysis of S. scardica infusion using ultra-high-performance liquid chromatography hyphenated with high-resolution mass spectrometry (UHPLC–HRMS). Quantitative determination of the main secondary metabolites was carried out by UHPLC–HRMS analyses using the external standard method. The results revealed more than 100 metabolites, including five sugar acids and saccharides, 21 carboxylic, hydroxybenzoic, hydroxycinnamic acids, and derivatives, 15 acylquinic acids, 10 phenylpropanoid glycosides, four iridoid glycosides, 28 flavonoids, seven fatty acids, and four organosulfur compounds. Furthermore, a dereplication and fragmentation patterns of five caffeic acids oligomers and four acylhexaric acids was performed for the first time in S. scardica. Regarding the quantitative analysis, the phenylethanoid verbascoside (53) (151.54 ± 10.86 mg/g lyophilized infusion, li), the glycosides of isoscutellarein (78) (151.70 ± 14.78 mg/g li), methylisoscutelarein (82) (107.4 ± 9.07 mg/g li), and hypolaetin (79) (78.33 ± 3.29 mg/g li), as well as caffeic acid (20) (87.25 ± 6.54 mg/g li), were found to be the major compounds in S. scardica infusion. The performed state-of-the-art phytochemical analysis of S. scardica provides additional knowledge for the chemical constituents and usage of this valuable medicinal plant.

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Immunomodulating polyphenols from Sideritis scardica

Cited by 8 publications

References 50 publications

Comparative Study on Assisted Solvent Extraction Techniques for the Extraction of Biologically Active Compounds from Sideritis raeseri and Sideritis scardica

Comparative Study on Assisted Solvent Extraction Techniques for the Extraction of Biologically Active Compounds from Sideritis raeseri and Sideritis scardica

Sideritis scardica Extracts Demonstrate Neuroprotective Activity against Aβ25–35 Toxicity

A Comprehensive Phytochemical Analysis of Sideritis scardica Infusion Using Orbitrap UHPLC-HRMS

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