Immunomodulation in Mice by Experimental Infection with <i>Yersinia enterooolitica</i>

Ruiz‐Bravo, Alfonso; Jiménez-Valera, Marı́a; Cienfuegos, Gerardo Álvarez de; Ruíz, Concepción; Kouwatli, Khalil; Ramos‐Cormenzana, A.

doi:10.1111/j.1348-0421.1985.tb00899.x

Cited by 17 publications

(8 citation statements)

References 26 publications

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“…with 5 )< 107 bacteria in 0.1 ml saline [9]. Experimental groups comprised at least 6 mice of 6-8 weeks of age.…”

Section: Mice and Experimental Infectionmentioning

confidence: 99%

“…Mice were killed 24 h after the eliciting treatment, and peritoneal cells were harvested as described previously [9], except that Hanks' solution with sodium heparinate was used; peritoneal leukocytes were counted in a hemocytometer chamber.…”

Section: Peritoneal Leucocyte Responsementioning

confidence: 99%

“…The ability to elicit an inflammatory response is considered as an important characteristic of many * To whom correspondence should be addressed. into mice [9]. Recently, we have described the adjuvancy of Y. enterocolitica and its induction of an inflammatory reaction when injected i.p.…”

Section: Introductionmentioning

confidence: 99%

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Role of bone marrow in inflammatory response to experimental Yersinia enterocolitica infection in mice

Ruiz-Bravo

1986

FEMS Microbiology Letters

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Mice infected intraperitoneally (i.p.) with Yersinia enterocolitica developed an inflammatory response, as revealed by a large influx of leukocytes in the peritoneal cavity. When the infection was preceded by the administration of Y. enterocolitica by the same route 4 days before, this resulted in a poor inflammatory reaction. On the other hand, the response of previously immunized animals to infection resembled to those of primoinfected mice. Bone marrow cellularity was decreased after the infection with Y. enterocolitica. Since bone marrow depletion by pre‐treatment with cyclophosphamide decreased the inflammatory response to Y. enterocolitica, we concluded that marrow cell reserve was necessary for the inflammatory reaction, whereas specific immunity did not affect this response.

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“…with 5 )< 107 bacteria in 0.1 ml saline [9]. Experimental groups comprised at least 6 mice of 6-8 weeks of age.…”

Section: Mice and Experimental Infectionmentioning

confidence: 99%

Section: Peritoneal Leucocyte Responsementioning

confidence: 99%

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Role of bone marrow in inflammatory response to experimental Yersinia enterocolitica infection in mice

Ruiz-Bravo

1986

FEMS Microbiology Letters

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“…We have previously demonstrated that low‐virulence Y. enterocolitica strains possess immunomodulatory properties [7–9]. Interestingly, experimental infection with IP383, a pYV‐bearing strain belonging to the low‐virulence serotype O9, confers on BALB/c mice significant resistance levels against transplantation of the syngeneic lymphoma LSTRA or challenge with a lethal dose of the intracellular pathogen Listeria monocytogenes [8,9].…”

Section: Introductionmentioning

confidence: 99%

Immunomodulation byYersinia enterocolitica: comparison of live and heat-killed bacteria

Ruiz‐Bravo

Bujalance

Romero

et al. 2003

FEMS Immunology &Amp; Medical Microbiology

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This study compared the immunomodulating properties of viable and killed Yersinia enterocolitica O9 in BALB/c mice. At 10 days after infection by the intragastric route, ex vivo assays showed a suppression of spleen cell proliferation in response to Salmonella lipopolysaccharide, concanavalin A and heat-killed yersiniae. Mice infected with Y. enterocolitica O9 for 10 days resisted the challenge with a lethal dose of Listeria monocytogenes. In contrast, intravenous administration of heat-killed yersiniae did not modify the ability of spleen cells to proliferate in response to lipopolysaccharide or concanavalin A, and proliferation in response to killed yersiniae was significantly increased. By 3 days after administration of a single dose of heat-killed yersiniae, the resistance of mice to L. monocytogenes challenge was significantly increased. Our findings show profound differences in immunomodulation by viable and heat-killed yersiniae, but suggest that killed yersiniae retain interesting immunomodulating properties.

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“…The effects of experimental yersiniosis on immunity parameters, such as the size of spleen cell subsets or the production of immune cytokines, have been documented in mice infected with serotype O8 strains (Autenrieth et al., 1994 ; Bohn et al, 1998). In comparison, lowvirulence serotypes have received little attention, although they possess immunomodulatory properties (Ruiz-Bravo et al, 1985. In humans, enteric colonization with low-virulence serotypes may persist for periods of up to 14 weeks without symptoms (Morris et al, 1991) ; however, there is no information available about the possible effects of the intestinal carriage of these strains on the immune condition of the hosts.…”

Section: Introductionmentioning

confidence: 99%

Intestinal infection of BALB/c mice with Yersinia enterocolitica O9 causes major modifications in phenotype and functions of spleen cells

2001

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INTRODUCTIONYersinia enterocolitica is a common cause of intestinal infections in humans (Cover & Aber, 1989 ;Bottone, 1997). Extraintestinal and systemic infections are most often caused by strains belonging to so-called highvirulence serotypes, such as serotype O8 (Heesemann et al., 1993), although low-virulence serotypes, such as O3 and O9, may occasionally cause bactaeremia in patients with iron overload or other underlying diseases (Rabson et al., 1975 ;Bouza et al., 1980). The presence of a 70 kb plasmid (pYV) that carries a number of virulence genes is required for Y. enterocolitica strains to become virulent (Cornelis et al., 1998).Non-septic sequelae of human yersiniosis include reactive arthritis, erythema nodosum, Reiter's syndrome, autoimmune thyroiditis and other immunopathological complications (Winblad, 1973 ;Bech et al., 1974 ;Toivanen et al., 1985). Alterations in the immune networks following infection with a number of microorganisms are likely to play a role in the genesis of immunopathological diseases (Schwartz, 1993). The effects of experimental yersiniosis on immunity parameters, such as the size of spleen cell subsets or the production of immune cytokines, have been documented in mice infected with serotype O8 strains (Autenrieth et al., 1994 ; Bohn et al., 1998). In comparison, lowvirulence serotypes have received little attention, although they possess immunomodulatory properties (Ruiz-Bravo et al., 1985. In humans, enteric colonization with low-virulence serotypes may persist for periods of up to 14 weeks without symptoms (Morris et al., 1991) ; however, there is no information available about the possible effects of the intestinal carriage of these strains on the immune condition of the hosts. In this study, we used a murine intestinal infection model (Ruiz-Bravo et al., 1999) A. RUIZ-BRAVO, E. MORENO and M. JIME; NEZ-VALERA of mice infected with a low-virulence serotype of Y. enterocolitica. METHODSMice. Six-to eight-week-old female BALB\c mice were provided by the Unit of Animal Experimentation, University of Granada (Granada, Spain). They were maintained under pathogen-free conditions. Experimental infection with Y. enterocolitica. Strain IP383 of Y. enterocolitica was used in all experiments. It is a serotype O9 strain that carries the virulence plasmid pYV (Mazigh et al., 1983). Bacteria were grown on Tryptic soy agar (Difco) at 25 mC for 24 h, harvested, washed and resuspended in sterile water to obtain 10* bacteria ml − ". Mice were infected via the oral route, as described previously (Ruiz-Bravo et al., 1999). Phenotypic analysis of spleen cells. Spleens were homogenized in PBS containing 1 % (w\v) BSA (Sigma). Splenocytes were sedimented by centrifugation, resuspended in red blood cell lysing buffer (Sigma) for 10 min, washed and resuspended in PBS containing BSA. Cell suspensions from individual mice were adjusted to 10( viable cells ml − ". Aliquots (100 µl) were incubated with 5 µl fluorescein-isothiocyanate-conjugated monoclonal antibodies. The monoclonal antibodies use...

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Immunomodulation in Mice by Experimental Infection with Yersinia enterooolitica

Cited by 17 publications

References 26 publications

Role of bone marrow in inflammatory response to experimental Yersinia enterocolitica infection in mice

Role of bone marrow in inflammatory response to experimental Yersinia enterocolitica infection in mice

Immunomodulation byYersinia enterocolitica: comparison of live and heat-killed bacteria

Intestinal infection of BALB/c mice with Yersinia enterocolitica O9 causes major modifications in phenotype and functions of spleen cells

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