Immunomodulation in Mytilus galloprovincialis by non-toxic doses of hexavalent Chromium

Ciacci, Caterina; Barmo, Cristina; Fabbri, Rita; Canonico, Barbara; Gallo, Gabriella; Canesi, Laura

doi:10.1016/j.fsi.2011.09.002

Cited by 46 publications

(24 citation statements)

References 49 publications

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“…Cellular immunity of Drosophila comprises hemocytes [41], therefore, the observed reduction in total hemocyte count in Cr(VI) exposed Drosophila indicates adverse impact of Cr(VI) on cellular immunity. This is corroboration with earlier studies on different model organisms [13], [42]. The altered hemocyte population in exposed Drosophila was found to be consistent with the down-regulation of hemese , which is expressed in the hemocytes of Drosophila larvae [43], in Cr(VI) exposed organism.…”

Section: Discussionsupporting

confidence: 91%

Over-Expression of Superoxide Dismutase Ameliorates Cr(VI) Induced Adverse Effects via Modulating Cellular Immune System of Drosophila melanogaster

et al. 2014

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The evolutionarily conserved innate immune system plays critical role for maintaining the health of an organism. However, a number of environmental chemicals including metals are known to exert adverse effects on immune system. The present study assessed the in vivo effect of a major environmental chemical, Cr(VI), on cellular immune response using Drosophila melanogaster and subsequently the protective role of superoxide dismutase (SOD) based on the comparable performance of the tested anti-oxidant enzymes. The immuno-modulatory potential of Cr(VI) was demonstrated by observing a significant reduction in the total hemocyte count along with impaired phagocytic activity in exposed organism. Concurrently, a significant increase in the percentage of Annexin V-FITC positive cells, activation of DEVDase activity, generation of free radical species along with inhibition of anti-oxidant enzyme activities was observed in the hemocytes of exposed organism. In addition, we have shown that ONOO− is primarily responsible for Cr(VI) induced adverse effects on Drosophila hemocytes along with O2 −. While generation of O2 −/ONOO− in Cr(VI) exposed Drosophila hemocytes was found to be responsible for the suppression of Drosophila cellular immune response, Cr(VI) induced alteration was significantly reduced by the over-expression of sod in Drosophila hemocytes. Overall, our results suggest that manipulation of one of the anti-oxidant genes, sod, benefits the organism from Cr(VI) induced alteration in cellular immunity. Further, this study demonstrates the applicability of D. melanogaster to examine the possible effects of environmental chemicals on innate immunity which can be extrapolated to higher organisms due to evolutionary conservation of innate immune system between Drosophila and mammals.

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Section: Discussionsupporting

confidence: 91%

Over-Expression of Superoxide Dismutase Ameliorates Cr(VI) Induced Adverse Effects via Modulating Cellular Immune System of Drosophila melanogaster

et al. 2014

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“…Using NR assay, the results showed that antidpressants induced a significant permeabilization of lysosomes in almost 100 % of the haemocytes at LC 50 . A decrease in the lysosomal membrane stability was also observed in M. galloprovincialis haemocytes exposed in vitro to different concentrations of chromium (0.1, 1, 10 and 100 lM) (Ciacci et al 2011). …”

Section: Cell Viabilitymentioning

confidence: 72%

The cell cultures and the use of haemocytes from marine molluscs for ecotoxicology assessment

Ladhar-Chaabouni

Hamza-Chaffai

2015

Cytotechnology

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Among aquatic organisms suitable for biological monitoring, molluscs occupy a prominent place due to their wide geographic distribution, their abundance and accessibility in the field as well as in aquaculture. Molluscs reflect the degree of environmental contamination and are the most useful bioindicator tools. The study of modulation of immune system or immunomodulation in marine molluscs has become one of the privileged ways for evaluating the physiological effects of environmental factors. Physiological responses of molluscs to environmental stresses could be mediated by haemocytes. These cells are continually exposed to the external environment due to the open circulatory system of molluscs and are affected by pollutants. In fact, several studies showed the effects of different environmental contaminants on haemocyte functions (viability, phagocytosis, ROS production) as well as on proteins involved in cytoskeletal structure maintenance using the in vitro approaches. In ecotoxicology, in vitro approach is an alternative to animal testing due to the reduced use of experimental animals, low cost and rapid performance. Although several studies showed the importance of using in vitro cell models to determine the effects of different environmental contaminants on haemocyte parameters in marine molluscs, a few reviews highlight these effects. The main purpose of this paper is to summarize the recent data on the effect of some xenobiotics on haemocyte parameters in some mollusc species and then suggest future research prospects.

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“…They reported that there was higher concentration of the metal in the haemolymph of the animal which had some significant effects on the hemocyte density and oxidative burst activity. Ciacci et al [62] also reported a decreased phagocytic activity and total hemocyte counts after exposing Mytilus galloprovincialis to Chromium concentrations ranging from 0.1-10 µg L -1…”

Section: Biochemical Biomarkers Immunotoxic/genotoxic Biomarkersmentioning

confidence: 94%

“…They reported more DNA damage in abalone kept in high oxygen, 7.7 mg/L at 19°C than 14°C and control, 16°C sections. According to Hooper et al [8], after subjecting hybrid Australian abalone to a thermal stress (26°C) from its normal 16°C, they observed a significant decline in antibacterial, phagocytic, phenoloxidase activities but high total haemocyte counts [62]. Leucine aminopeptidase activity between the experimental and control treatments was not significantly different.…”

Section: Biochemical Biomarkers Immunotoxic/genotoxic Biomarkersmentioning

confidence: 97%

Physiological, Immunological, Genotoxic and Histopathological Biomarker Responses of Molluscs to Heavy Metal and Water-Quality Parameter Exposures: A Critical Review

Os¹

2017

J Oceanogr Mar Res

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Immunomodulation in Mytilus galloprovincialis by non-toxic doses of hexavalent Chromium

Cited by 46 publications

References 49 publications

Over-Expression of Superoxide Dismutase Ameliorates Cr(VI) Induced Adverse Effects via Modulating Cellular Immune System of Drosophila melanogaster

Over-Expression of Superoxide Dismutase Ameliorates Cr(VI) Induced Adverse Effects via Modulating Cellular Immune System of Drosophila melanogaster

The cell cultures and the use of haemocytes from marine molluscs for ecotoxicology assessment

Physiological, Immunological, Genotoxic and Histopathological Biomarker Responses of Molluscs to Heavy Metal and Water-Quality Parameter Exposures: A Critical Review

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