Immunomodulator Activity of Puguntano (Picria fel-terrae Lour.) Extract in White Male Mice By Carbon Clearance Method

Utama, Rezza Fikrih; Rosidah,; Yuandani, Yuandani

doi:10.32734/idjpcr.v3i2.4306

idjpcr

2020

DOI: 10.32734/idjpcr.v3i2.4306

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Immunomodulator Activity of Puguntano (Picria fel-terrae Lour.) Extract in White Male Mice By Carbon Clearance Method

Rezza Fikrih Utama

Rosidah Rosidah

Yuandani Yuandani

Abstract: Poguntano Herb Plants (Picria fel-terrae Lour.) contains flavonoids compounds that have been potentially to developed into immunomodulators. The purpose of this study was to determine the immunomodulatory activity of Poguntano herb extract (Picria fel-terrae Lour.), with the method of carbon clearance. n-hexane extract puguntano herb(ENHHP), extract of ethyl acetate of Poguntano herb (EEAHP), and ethanol extract of puguntano herb(EEHP) Poguntano multistage results in the test of immunomodulatory activity with … Show more

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“…test preparation to excite the non-specific immune system in phagocytosing carbon ink colloidal particles(Utama, Rosidah and Yuandani, 2020).…”

mentioning

confidence: 99%

The effect of natural ingredients extracts on the phagocytosis index of the carbon clearance animal models

Vikasari

2024

jai

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The immune system has a very important role to fight foreign substances in the body. Immunostimulants are needed to stimulate the activity of the immune system and immunosuppressants are needed to suppress the immune system. The aims of this study is to evaluate phagocitytosis index of several natural ingredient extract using carbon clearance method. The natural ingredients are the ethanol extract of Ageratum conyzoides leaves 160 mg/kgBW, ethanol extract of Artocarpus heterophyllus leaves 500 mg/kgBW, ethanol extract of Nephelium lappaceum peel 650 mg/kgBW, ethanol extract of Cucurbita moschata seed 342 mg/kgBW and ethanol extract of Citrullus lanatus rind 55 mg/kgBW. Tests were carried out on male mice Balb/C strains using the carbon clearance method, where colloidal carbon ink acts as an antigen. The phagocytic activity of macrophages in eliminating carbon ink was measured based on the stimulation index value and then the immunomodulatory effect was classified according to Wagner's criteria. The stimulation index of A.heterophyllus was 1.423; A. conyzoides was 1.201; N. lappaceum was 1.236; C.lanatus was 0.909 and C. moschata was 1.299. The potential extract as immunostimulant was A.heterophyllus, as immunosupressant was C. lanatus. A.conyzoides and probably had no effect (act as imunorestorant) and C. moschata 342 mg/kgBW had slight immunostimulant effect.

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“…test preparation to excite the non-specific immune system in phagocytosing carbon ink colloidal particles(Utama, Rosidah and Yuandani, 2020).…”

mentioning

confidence: 99%

The effect of natural ingredients extracts on the phagocytosis index of the carbon clearance animal models

Vikasari

2024

jai

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Immunomodulatory Effects of Combined Ethanol Extracts of Curcuma mangga and Picria fel-terrae on Cellular- and Humoral-Mediated Immunity in Wistar Rats and Mice

Yuandani

Jantan

Laila

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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Previous studies have shown that the extracts of Curcuma mangga Valeton & Zijp rhizomes and Picria fel-terrae Lour. leaves could modulate cellular- and humoral-mediated immunity in macrophages and animal models. In the present study, the immunomodulatory effects of combined ethanol extracts of C. mangga rhizomes and P. fel-terrae leaves were investigated on cellular- and humoral-mediated immunity in Wistar rats and mice. The phytochemical constituents of the ethanol extracts of C. mangga and P. fel-terrae, and combined extracts were analyzed by high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). Mice were orally administered with combined extracts of C. mangga and P. fel-terrae (1 : 1) at doses of 25, 50, and 100 mg/kg·bw for 7 days, and the carbon clearance method was used to investigate their phagocytosis activity. Wistar rats were treated orally with the combined extracts 72 h prior to sensitization with Staphylococcus aureus and continued for 14 days. The effect of extracts on delayed-type hypersensitivity (DTH) response was determined by the paw edema method, while the effects on antibody (IgG and IgM) and interleukin-2 (IL-2) production were analyzed using enzyme-linked immunosorbent assay (ELISA). Picfeltarraenin VI and ferruginol were the major components in the extracts of P. fel-terrae and C. mangga, respectively. The combined extracts at 1 : 1 ratio demonstrated a dose-dependent stimulation of both cellular- and humoral-mediated immunity in both animal models. The combined extracts displayed the strongest stimulation on DTH response and phagocytosis activity at 100 mg/kg·bw, which were comparable with those of the positive control, levamisole. IgG and IgM production and IL-2 release were also stimulated after treatment with extracts. The combined extracts of C. mangga and P. fel-terrae possess strong stimulatory activities on cellular- and humoral-mediated immunity and may be developed as a potential nutraceutical for the modulation of immune responses.

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Immunomodulator Activity of Puguntano (Picria fel-terrae Lour.) Extract in White Male Mice By Carbon Clearance Method

Cited by 2 publications

References 9 publications

The effect of natural ingredients extracts on the phagocytosis index of the carbon clearance animal models

The effect of natural ingredients extracts on the phagocytosis index of the carbon clearance animal models

Immunomodulatory Effects of Combined Ethanol Extracts of Curcuma mangga and Picria fel-terrae on Cellular- and Humoral-Mediated Immunity in Wistar Rats and Mice

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