Immunomodulator Effect of Picroliv and its Potential in Treatment Against Resistant Plasmodium yoelii (MDR) Infection in Mice

Dwivedi, Varun; Khan, Mukhtar A.; Vasco, Azevedo; Fatima, Nishat; Soni, Vikas; Dangi, Anil; Misra‐Bhattacharya, Shailja; Owais, Mohammad

doi:10.1007/s11095-008-9631-2

Cited by 8 publications

(5 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This potentially makes the Nigella sativa oil extract a more likely antimalarial agent as an adjuvant. The present finding is consistent with the work of Dwived et al .,[10] in which they reported that the co-administration of CQ and an immune stimulant Picrurhiza kurrua extract enhanced the efficacy of CQ in murine mice. Also, studies of co-administration of various antimalarial tested medicinal plant extracts in Madagascar and Kenya revealed a reversal in chloroquine resistance in animals.…”

Section: Discussionsupporting

confidence: 94%

“…[7] Complementary use of antioxidants of natural origin with conventional antimalarial like chloroquine has been used in the past, particularly with those whose safety and efficacies are known and are employed in the treatment of malaria as an adjunct. [8910] The use of an antioxidant will undoubtedly reduce the oxidative stress caused by parasite and chloroquine. Hence, improving host antioxidant status will help to decrease parasite load by suppressing parasite multiplication.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Dietary supplementation of chloroquine with nigella sativa seed and oil extracts in the treatment of malaria induced in mice with plasmodium berghei

et al. 2014

View full text Add to dashboard Cite

Background:The aim of the study was to investigate the effects of dietary combination of Nigella sativa seed and oil extracts with chloroquine (CQ), and how these combinations enhance CQ efficacy in mice infected with Plasmodium berghei and their survival rates.Materials and Methods:Chloroquine sensitive P. berghei, NK65 strain was used for the study. This was passaged intraperitoneally into albino mice with a 0.2ml standard inoculum consisting of 106 parasitized erythrocyte suspension in phosphate buffer solution (PBS). Parasitaemia was ascertained by microscopical examination of blood films under oil immersion at X100 magnification.Results:Nigella sativa seed in feed (NSSF), NSSF + CQ on day 4, produced 86.1% and 86.0% suppression respectively, while Nigella sativa oil extract in feed (NSOF) and in combination with CQ had 86.0% and 99.9% suppression respectively. The degree of suppression with the combination was significantly higher compared to CQ alone (P < 0.001) (36.1%). Complete parasitaemia clearance was obtained on the 20th and 15th day of treatment for NSSF, NSSF + CQ respectively, while that for NSOF and NSOF + CQ was on days 26 and 12 respectively. For CQ parasite clearance was 12 days with treatment. Also, the combinastion of 10 mg/kg Nigella sativa oil treatment injected intraperitoneally with oral CQ produced very significant parasite suppression (P < 0.0001) (93%). Survival rate in NSSF and NSOF and in combination with CQ groups was 100 and 60.0% for CQ alone.Conclusions:This study shows that the use of Nigella sativa seed and oil extract as dietary supplements in combination with CQ has a potential in enhancing the efficacy of CQ and could be of benefit in management of malaria.

show abstract

Section: Discussionsupporting

confidence: 94%

Section: Introductionmentioning

confidence: 99%

Dietary supplementation of chloroquine with nigella sativa seed and oil extracts in the treatment of malaria induced in mice with plasmodium berghei

et al. 2014

View full text Add to dashboard Cite

show abstract

“…Picroliv, a standardized fraction isolated from the ethanol extract of the root and the rhizome of Picrorhiza kurroa (Scrophulariaceae) has been reported to activate the host immune system. Co-administration of Picroliv was found to enhance efficacy of chloroquine against experimental murine malaria [64]. A traditional Chinese mixture known as Juzen-taiho-to suppressed murine malaria by increasing the production of antibodies and interferon gamma [65].…”

Section: Complementary Mechanisms Of Actionmentioning

confidence: 99%

Whole plant extracts versus single compounds for the treatment of malaria: synergy and positive interactions

Rasoanaivo

Wright

Willcox

et al. 2011

Malar J

422

262

View full text Add to dashboard Cite

BackgroundIn traditional medicine whole plants or mixtures of plants are used rather than isolated compounds. There is evidence that crude plant extracts often have greater in vitro or/and in vivo antiplasmodial activity than isolated constituents at an equivalent dose. The aim of this paper is to review positive interactions between components of whole plant extracts, which may explain this.MethodsNarrative review.ResultsThere is evidence for several different types of positive interactions between different components of medicinal plants used in the treatment of malaria. Pharmacodynamic synergy has been demonstrated between the Cinchona alkaloids and between various plant extracts traditionally combined. Pharmacokinetic interactions occur, for example between constituents of Artemisia annua tea so that its artemisinin is more rapidly absorbed than the pure drug. Some plant extracts may have an immunomodulatory effect as well as a direct antiplasmodial effect. Several extracts contain multidrug resistance inhibitors, although none of these has been tested clinically in malaria. Some plant constituents are added mainly to attenuate the side-effects of others, for example ginger to prevent nausea.ConclusionsMore clinical research is needed on all types of interaction between plant constituents. This could include clinical trials of combinations of pure compounds (such as artemisinin + curcumin + piperine) and of combinations of herbal remedies (such as Artemisia annua leaves + Curcuma longa root + Piper nigum seeds). The former may enhance the activity of existing pharmaceutical preparations, and the latter may improve the effectiveness of existing herbal remedies for use in remote areas where modern drugs are unavailable.

show abstract

“…About 100-200 g of fresh leaves or shoots of khat are chewed per session and juice of the khat is swallowed while the plant residue is retained in mouth [ 5 ]. Khat induces a mild euphoric state giving the chewer a feeling of being more focused, energetic and communicative [ 1 , 4 ].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of immunomodulatory activities of methanolic extract of khat (Catha edulis, Forsk) and cathinone in Swiss albino mice

et al. 2015

View full text Add to dashboard Cite

BackgroundThe objective of this study was to explore the immunomodulatory effect of khat and its active component, cathinone, using mice.Materials and methodsFemale Swiss albino mice aged 7–8 weeks weighing 25-30 g were used in the study. Mice were randomized into eight groups of 6 each and oral treatment of khat crude extract and cathinone were given daily for four weeks. Physical, hematological, biochemical, and immunological parameters were measured. Immunological studies included humeral immunity (IgG and IgM), cellular immune response (delay type hypersensitivity), phagocytic activities of reticuloendothelial system, and determination of T-lymphocyte population: CD3+, CD4+, CD8+ count and CD4+/CD8+ status.ResultsFindings of this study showed that, khat and its major metabolite, cathinone, can positively affect immune system in dose dependent manner. When doses of crude khat extract and cathinone increase, the induction of humeral (IgG and IgM) and cellular immune responses were up-regulated significantly (P < 0.05), while at higher dose of khat (200 mg/kg) cellular immune response was suppressed. In support of this, as doses of the two test substances increased, the count of T helper cells (CD4+) was significantly increased (P < 0.05), while higher dose significantly reduced whole white blood cell (WBC), CD8+, and CD3+ counts.ConclusionAt relatively lower dose (50-100 mg/kg), crude khat extract has immune stimulating property, although higher dose (200 mg/kg) leads to suppression of cellular immune response. Cathinone also share all immune modulating property of its parent compound, khat, but with intense strength. Thus, it calls for further detailed investigation of khat for practical application of the same in human medication.Electronic supplementary materialThe online version of this article (doi:10.1186/s12865-015-0072-5) contains supplementary material, which is available to authorized users.

show abstract

Immunomodulator Effect of Picroliv and its Potential in Treatment Against Resistant Plasmodium yoelii (MDR) Infection in Mice

Abstract: The activation of host immune system can increase the efficacy of chloroquine for suppression of drug resistant malaria infection in BALB/c mice.

Cited by 8 publications

References 26 publications

Dietary supplementation of chloroquine with nigella sativa seed and oil extracts in the treatment of malaria induced in mice with plasmodium berghei

Dietary supplementation of chloroquine with nigella sativa seed and oil extracts in the treatment of malaria induced in mice with plasmodium berghei

Whole plant extracts versus single compounds for the treatment of malaria: synergy and positive interactions

Evaluation of immunomodulatory activities of methanolic extract of khat (Catha edulis, Forsk) and cathinone in Swiss albino mice

Contact Info

Product

Resources

About