Immunomodulatory Activities of Body Wall Fatty Acids Extracted from <i>Halocynthia aurantium</i> on RAW264.7 Cells

Monmai, Chaiwat; Jang, A-yeong; Kim, Jieun; Lee, Sang‐Min; You, SangGuan; Kang, SeokBeom; Park, Woo Jung

doi:10.4014/jmb.2007.07032

Cited by 8 publications

(10 citation statements)

References 46 publications

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“…This implies that a full PLANT diet could provide more vitamin B6 and vitamin PP than the COMB and SILAGE diets. Arachidonic acid is the precursor for the synthesis of prostaglandin, thromboxane, and leukotriene derivatives and plays an important role in the cardiovascular and immune systems of animals [ 46 , 47 ]. However, the PLANT diet has a relatively low starch content, resulting from significantly decreased quantities of maltotriose.…”

Section: Discussionmentioning

confidence: 99%

Effect of Different Dietary Regimes on the Gut Microbiota and Fecal Metabolites of Père David’s Deer

Zhen

Ren

Zhang

et al. 2022

Animals

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A deep understanding of the effect of seasonal dietary changes on the nutrition and health of Père David’s deer in Dafeng Reserve will contribute greatly to Père David’s deer’s protection. In this reserve, there were three seasonal dietary regimes: feeding on naturally occurring plants (PLANT diet), silage (SILAGE diet), and a combination of natural plants and silage (COMB diet). To some extent, the COMB diet reflects the seasonal transition from silage to the all-natural plant diet, especially in early spring. However, little is known regarding the gut microbiota changes and metabolic consequences under the COMB diet. Based on 16S rRNA sequencing and ultra-high performance liquid chromatography combined with tandem mass spectrometry, the gut microbiota and fecal metabolites of Père David’s deer under these three diets were compared. Results showed the alpha diversity of the gut microbiota was significantly lower under the COMB diet compared to either the SILAGE or PLANT diets. Although no significant changes were observed in the core phyla, Firmicutes and Bacteroidetes, among the three dietary regimes, a significant lower abundance of several other phyla (Spirochaetes, Melainabacteria, Proteobacteria, and Verrucobacteria) was observed in the COMB diet compared to the SILAGE diet. A greater number of fecal metabolite differences was identified between the COMB and SILAGE or COMB and PLANT diets than between the SILAGE and PLANT diets, suggesting that the COMB diet had more of an effect on the metabolism of Père David’s deer. The integrated pathway analysis showed that several metabolic pathways were significantly affected by the different dietary regimes, such as tryptophan metabolism, vitamin metabolism, and the platelet activation pathways. These metabolic changes reflect the responses and adaptations of Père David’s deer to different diets. Taken overall, our data reveal the difference in the gut microbiota and metabolic pathways of Père David’s deer under three dietary regimes in Dafeng Reserve, which provides important information for Père David’s deer conservation.

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Section: Discussionmentioning

confidence: 99%

Effect of Different Dietary Regimes on the Gut Microbiota and Fecal Metabolites of Père David’s Deer

Zhen

Ren

Zhang

et al. 2022

Animals

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“…CCP increased the expression of COXs especially COX-2 in the gene level, and thus leading to the accumulation of PGE 2 and PGD 2 ( Figure S7 ). PGE 2 plays an important role in immune response and it stimulates secretion of cytokines (such as TNF-α and IL-6) which regulates the innate immunity ( Monmai et al, 2020 ). PGE 2 can also exert an immunomodulatory effect by promoting macrophages phenotype conversion and by modulating regulatory T cells development and function ( AN et al, 2018 ).…”

Section: Resultsmentioning

confidence: 99%

A new polysaccharide from Caulerpa chemnitzia induces molecular shifts of immunomodulation on macrophages RAW264.7

Hao

et al. 2022

Food Chemistry: X

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“…The PCR reaction included 5 ng of cDNA template and 0.375 µM of each specific primer and was conducted as follows: predenaturation (95 °C for 10 min), 40 cycles of PCR (denaturation: 95 °C for 20 s; annealing: 60 °C for 20 s; extension: 72 °C for 30 s), and final extension (72 °C for 5 min). The sequences of IL-1β , IL-6 , COX-2 , iNOS , TNF-α , and β-actin were used as described by Monmai et al [ 25 ]; moreover, the sequences of TLR-4 (NM_021297.3; forward: 5′-CGC TCT GGC ATC ATC TTC AT-3’; reverse: 5′-GTT GCC GTT TCT TGT TCT TCC-3′) were used. The results were analyzed using CFX Maestro software, and β-actin was used as the reference gene.…”

Section: Methodsmentioning

confidence: 99%

Transgenic Rice Seed Extracts Exert Immunomodulatory Effects by Modulating Immune-Related Biomarkers in RAW264.7 Macrophage Cells

2022

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Protopanaxadiol (PPD), a native active triterpenoid present in Panax ginseng, has been reported to exert immune-related effects. We previously created PPD-producing transgenic rice by introducing the P. ginseng protopanaxadiol synthase and dammarenediol-II synthase genes into Dongjin rice. In the present study, the seeds of the T4 generation of this transgenic rice were tested for their immunomodulatory effects in RAW264.7 macrophage cells. Treatment with transgenic rice seed extract in RAW264.7 cells (i) significantly enhanced nitric oxide (NO) production in a dose-dependent manner without any cytotoxicity (up to 100 µg/mL), (ii) upregulated the expression of immune-related genes and increased production of the inflammation mediator prostaglandin E2 (PGE2), and (iii) activated nuclear factor-κB (NF-κB) and mitogen-activated protein kinase (MAPK) by promoting the phosphorylation of NF-κB p65, p38 MAPK, and c-Jun N-terminal protein kinase (JNK). In lipopolysaccharide (LPS)-treated RAW264.7 cells used to mimic the inflammation condition, treatment with transgenic rice seed extract significantly reduced NO production, proinflammatory cytokine expression, and PGE2 production, all of which are LPS-induced inflammation biomarkers, by inhibiting the phosphorylation of NF-κB p65, p38 MAPK, and JNK. Collectively, these results indicate that PPD-producing transgenic rice has immunomodulatory effects.

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Immunomodulatory Activities of Body Wall Fatty Acids Extracted from Halocynthia aurantium on RAW264.7 Cells

Cited by 8 publications

References 46 publications

Effect of Different Dietary Regimes on the Gut Microbiota and Fecal Metabolites of Père David’s Deer

Effect of Different Dietary Regimes on the Gut Microbiota and Fecal Metabolites of Père David’s Deer

A new polysaccharide from Caulerpa chemnitzia induces molecular shifts of immunomodulation on macrophages RAW264.7

Transgenic Rice Seed Extracts Exert Immunomodulatory Effects by Modulating Immune-Related Biomarkers in RAW264.7 Macrophage Cells

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