Immunomodulatory activity of polyphenols derived from Cassia auriculata flowers in aged rats

Abstract

The immunomodulatory activity of Cassia auriculata (CA)-derived polyphenols was tested on aged rats. Rats (24-26 months old) were given CA polyphenols supplementation at doses of 25, 50, and 100 mg/kg for 28 days. Flow cytometry analysis of CA polyphenols-treated aged rats showed increased T and B cells percentage along with enhanced proliferation of splenocytes in both resting and LPS-stimulated cells. Increased percentage of pan T cells is further supported by an elevation of CD4+, CD8+, and CD4+CD25+ regulatory cells. In terms of innate immune cell activity, CA polyphenol supplementation reduced the oxidative burst activity of neutrophils in response to PMA and Escherichia coli activation. Our results collectively show that polyphenols derived from CA boost T cell immunity by increasing the number of T cells and its sensitivity towards stimulants and decreasing ROS production by neutrophils that could potentially harm multiple biological systems in aged individuals.

Antiinflammatory and antioxidant activities of a polyphenol‐rich extract fromZizyphus lotusL fruit pulp play a protective role against obesity

“…Hence, PolyP exerted immunomodulatory effects, i.e., decreased the level of pro‐inflammatory cytokines with increase in antiinflammatory cytokines. Our observation is in agreement with a previous study by John, Sandrasaigaran, Tong, Adam, & Ramasamy, , who have also shown the immunomodulatory activity of polyphenols derived from Cassia auriculata flowers in aged rats (John et al, ). Furthermore, PolyP significantly curtailed LPS‐induced mRNA expression of iNOS and production of NO.…”

Modulation of Immune Function by Polyphenols: Possible Contribution of Epigenetic Factors

“…In mice receiving oral treatment with polyphenols rich extracts from date palm tree, an increment of the immunocompetent cells, incluinding T helper 1 (Th1), natural killer (NK), macrophages and dendritic cells (DCs) in both Peyer’s patches and spleen [12] was observed. Similar effects were obtained in response to treatment of aged rats with polyphenols from Cassia auriculata , increasing splenic T and B cells [13]. Other studies have been reported mechanisms associated to specific cell groups among the different immune cells modulated by polyphenols.…”

“…Many studies support the immune modulation actions by polyphenols from diverse sources, describing immune modulator effects on different populations of immune cells [12,13]. In mice receiving oral treatment with polyphenols rich extracts from date palm tree, an increment of the immunocompetent cells, incluinding T helper 1 (Th1), natural killer (NK), macrophages and dendritic cells (DCs) in both Peyer’s patches and spleen [12] was observed.…”

(Anti)mutagenic and immunomodulatory properties of quercetin glycosides

“…T cell subsets. This correlates well with the results of Karasawa et al , who observed an incremental increase in the immunocompetent T helper 1 (Th1) cells, NK cells, macrophages and dendritic cells in both Peyer's patches in the gut‐associated lymphoid tissue (GALT) and in germinal centers of the spleen, and John et al , who described an increase in T and B splenic cells in aged rats, both in response to the treatment of experimental animals with polyphenols. Zunino and Storms described an increase in proliferation and immunoglobulin synthesis in B cells in adult individuals treated with pokeweed mitogen (a stimulator of B cells) as a consequence of treatment with resveratrol.…”