Immunomodulatory activity of polysaccharides from Brassica rapa by activating Akt/NF-κB signaling

Guo, Weiwei; Qianxiao, Zhang; Du, Yu; Guo, Junting; Zhao, Tingting; Bai, Liping; XiQiang, An

doi:10.1016/j.chmed.2021.10.003

Cited by 12 publications

(11 citation statements)

References 33 publications

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“…This stimulation of immunity cell proliferation was probably mediated by a lectin (glycoprotein) which is present in vegetal seeds and particularly in B. rapa as indicated by Raval et al, 2004 24 and for Lepidium sativum (Brassicaceae specie). 10 Similar immunostimulation of cells was also reported by Wang et al, 2021 12 by measuring the weight of the spleen and thymus under polysaccharides from B. rapa. Another study 32 with petroleum extract from B. rapa showed an immunostimulatory effect by increasing the proportions of B cells, CD4+ T cells, CD8+ T cells, and activated CD8+ T cells in the spleens of tumor-bearing mice.…”

Section: Discussionsupporting

confidence: 77%

“…26 On isolated macrophages, ethanolic and ethyl acetate extracts of B. rapa seeds both inhibited macrophage proliferation. In contrast, other authors observed stimulation of macrophages under Brassica roots extract 12,28 or by aqueous extract of total plant from Brassica campestris with the experiments conducted on macrophage cell line RAW 264.7 and not on isolated macrophages 11 We indicated, for the first time, an inhibition of freshly isolated macrophage proliferation under B. rapa seeds extracts leading to an antiinflammatory effect of compounds present in B. rapa extracts. This conclusion is confirmed by previous publications indicating an antiinflammatory effect of B. rapa root compounds on cultured macrophage cell lines.…”

Section: Discussioncontrasting

confidence: 69%

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Immunostimulant effect of Brassica rapa and Raphanus sativus seeds on thymic cells and their cytotoxicity

Laaraj

Ouahidi

Aarab

2023

EJI

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Immunomodulators from natural products are widely used for the treatment of infectious diseases, allergies, and cancer in traditional Moroccan medicine. The purpose of this study was the study of two plant seeds of Brassica rapa (Turnip) and Raphanus sativus (Radish) used by Moroccans in traditional medicine to enhance immunity. We have prepared three different extracts from seeds using ethanol, Ethyl Acetate, or water. Immunomodulatory effects of these two plant seeds were tested on rabbit immunity cell proliferation (splenocytes, thymocytes, and macrophages) and their functions (IgG production, cytotoxicity, and phagocytosis). The results obtained indicated that only aqueous extract of B. rapa seeds revealed an immunostimulant effect on both splenocyte and thymocyte proliferation with an increase in cytotoxicity of thymocytes (MLR assay). With R. sativus seeds, we observed an important stimulation of thymocyte proliferation and their cytotoxicity under aqueous extract without effect on splenocyte or macrophages. We concluded that aqueous extract of both seeds (B. rapa and R. sativus) possessed immunostimulant properties leading to stimulation of cellular immunity responsible for defense against viruses.

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Section: Discussionsupporting

confidence: 77%

Section: Discussioncontrasting

confidence: 69%

Immunostimulant effect of Brassica rapa and Raphanus sativus seeds on thymic cells and their cytotoxicity

Laaraj

Ouahidi

Aarab

2023

EJI

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“…B. rapa has been clinically proven to have anti‐tumor and immunity‐enhancing activity (Sun et al, 2010; Xiao & Zhang, 2010; Zhang, Ma & Zhang, 2013), and the polysaccharides of B. rapa (BRP) have been proved to exhibit antioxidative and immunomodulatory effects on RAW 264.7 cells. Our previous study has demonstrated that BRP possesses phagocytic capacity and has potent immunomodulatory activity via the activation of Akt and NF‐κB (Guo et al, 2022). In the present study, BRP has been shown to exhibit some anti‐tumor effect, while it has no direct inhibition on the growth of 4 T1 and LLC tumor cells.…”

Section: Discussionmentioning

confidence: 99%

“…Besides of STAT1, NF‐κB participates in M1 macrophage polarization. We previously demonstrated that BRP exerted immunomodulatory activities in RAW264.7 cells via activation of NF‐κB signaling pathways (Guo et al, 2022). In this study, we found that the increasing expressions of M1 marker genes and STAT1 phosphorylation upon BRP treatment were reversed via blockade of NF‐κB signaling pathway by the treatment with the NF‐κB inhibitor BAY11‐7082.…”

Section: Discussionmentioning

confidence: 99%

“…B. rapa was collected in April 2015 from Keping county, Xinjiang Uyghur Autonomous Region (79_2048''E, 40_30030''N), and identified by associate professor Jiang He (Xinjiang Institute of Materia Medica). BRP was extracted and isolated from the water extraction and EtOH (95%) precipitation of the dry powder of root of B. rapa using the previously described protocol, which comprised three main fractions analyzed by size exclusive chromatograph (Guo et al, 2022).…”

Section: Methodsmentioning

confidence: 99%

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Polysaccharides of Brassica rapa L. attenuate tumor growth via shifting macrophages to M1‐like phenotype

Guo

Liu

Guo

et al. 2022

Phytotherapy Research

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Macrophages are the major tumor‐infiltrating leukocytes, and tumor‐associated macrophages (TAM) play a critical role in cancer‐related inflammation since they show alternative polarization to M1 (tumor‐inhibited macrophages) or M2 (tumor‐promoted macrophages) phenotype. Brassica rapa L. (B. rapa) has been clinically proven to have anti‐tumor and immunity‐enhancing activity, and the polysaccharides of B. rapa (BRP) have been reported to have an immunoregulatory effect on macrophages. In this study, we focus on macrophage polarization to investigate the mechanism of anti‐tumor response of BRP in vivo and in vitro. We found that BRP improved the expression of M1 markers, including iNOS, COX‐2, HLA‐DR, CD11b and M1‐related cytokines. The expression of M2 markers Arg‐1, CD206 and CD163 induced by IL‐4 were inhibited by BRP treatment, resulting in the inhibition of tumor growth both in vivo and in co‐culture experiments in vitro. The activation of STAT signaling pathway were significantly regulated by BRP, which are important signals in TAM polarization. Overall, the results indicated that BRP has anti‐tumor effect through mediating macrophage polarization.

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Prophylactic Effects of Astragalus Polysaccharides on Depression‐Like Behaviors in Rats With Chronic Unpredictable Mild Stress: A Role of Gut‐Microbiota–Brain Axis

Liu,

Xu,

Zhao

et al. 2024

Food Frontiers

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Radix Astragalus (RA) has been used in the treatment of depression. Astragalus polysaccharide (APS) is one of the effective components of RA. However, the antidepressant mechanism of APS is still unclear. This research utilized 16S rRNA analysis, microbial analysis, and metabolomics analysis to elucidate the antidepressant mechanism of APS at both micro and macro levels. Correlation analysis was performed on the perspective of “behavioral indicators‐intestinal bacteria‐immune factors‐differential metabolites” to show the relationships among various indicators. The results showed that APS could significantly regulate the depressive behaviors of depressed rats, alleviate the imbalance of Th17/Treg, increase the expression level of anti‐inflammatory factor IL‐10, while reduce the expression level of pro‐inflammatory factor IL‐22. APS significantly reduced the expression levels of lysine, alanine, and arginine of depressed rats, as well as the abundance of Aerococcus. Lysine and arginine were the most closely related to behaviors, immune factors, and gut bacteria. The current findings revealed the antidepressant mechanism of APS from the perspective of the “gut microbiome–immunity–metabolome” axis. This study provides a new strategy for proving the antidepressant effects of plant polysaccharides and lays a solid foundation for the discovery of new drugs from botanical drugs and the improvement of patients' quality of life.

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Immunomodulatory activity of polysaccharides from Brassica rapa by activating Akt/NF-κB signaling

Cited by 12 publications

References 33 publications

Immunostimulant effect of Brassica rapa and Raphanus sativus seeds on thymic cells and their cytotoxicity

Immunostimulant effect of Brassica rapa and Raphanus sativus seeds on thymic cells and their cytotoxicity

Polysaccharides of Brassica rapa L. attenuate tumor growth via shifting macrophages to M1‐like phenotype

Prophylactic Effects of Astragalus Polysaccharides on Depression‐Like Behaviors in Rats With Chronic Unpredictable Mild Stress: A Role of Gut‐Microbiota–Brain Axis

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