Immunomodulatory and anti-infective effects of Cratylia mollis lectin (Cramoll) in a model of wound infection induced by Staphylococcus aureus

Carneiro, Mariela Andrea Medeiros Suarez; Silva, Lucas dos Santos; Diniz, Roseana Muniz; Saminez, Warlison Felipe da Silva; Oliveira, Patrícia Vieira de; Mendonça, Juliana Silva Pereira; Colasso, Arthur Henrique Mendes; Silva, Izadora Souza Soeiro; Jandú, Jannyson José Braz; Sá, Joicy Cortez de; Figueiredo, Cristiane Santos Silva e Silva; Correia, Maria Tereza dos Santos; Silva, Luís Cláudio Nascimento da

doi:10.1016/j.intimp.2021.108094

Cited by 13 publications

(5 citation statements)

References 51 publications

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“…TNF-α is strongly associated with the severity of skin infections caused by S. aureus [ 34 ]. Studies have shown that Cratylia mollis lectin significantly improved the severity of S. aureus infected lesions by inhibiting the expression of TNF-α in the lesions [ 35 ]. Similarly, in this study we demonstrated that topical treatment of juglone also significantly inhibited the level of IL-6 and IL-1β in mice, which is consistent with previous studies showing that juglone has anti-inflammatory effects [ 7 ].…”

Section: Discussionmentioning

confidence: 99%

“…Animal experiments were approved by the Animal Protection and Utilization Committee of Northwest Agriculture and Forestry University and were conducted following the guidelines of the Animal Protection and National Institutes of Health [ 35 ]. Twenty-five healthy Kunming mice (six to eight weeks old) were housed in a ventilated room with an average temperature of 21 °C and a light cycle of 12 h, with free access to food and water.…”

Section: Methodsmentioning

confidence: 99%

“…The histological assessment method was as described by Suarez Carneiro et al with minor modifications [ 35 ]. Skin fragments (covering experimentally induced lesion areas and intact skin) were collected at 14 dpi (14 d after the wound was inoculated with S. aureus (ATCC29213).…”

Section: Methodsmentioning

confidence: 99%

“…We referred to the method described by Suarez Carneiro et al [ 35 ]. Wound skin tissue was collected at 2 dpi and placed in 1 mL of PBS, vortexed for five cycles (30 s each), and then centrifuged at 3000 rpm for 5 min.…”

Section: Methodsmentioning

confidence: 99%

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Antibacterial Activity of Juglone Revealed in a Wound Model of Staphylococcus aureus Infection

Wan

Wang

Liu

et al. 2023

IJMS

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A serious problem currently facing the field of wound healing is bacterial infection, especially Staphylococcus aureus (S. aureus) infection. Although the application of antibiotics has achieved good effects, their irregular use has resulted in the emergence of drug-resistant strains. It is thus the purpose of this study to analyze whether the naturally extracted phenolic compound, juglone, can inhibit S. aureus in wound infection. The results show that the minimum inhibitory concentration (MIC) of juglone against S. aureus was 1000 μg/mL. Juglone inhibited the growth of S. aureus by inhibiting membrane integrity and causing protein leakage. At sub-inhibitory concentrations, juglone inhibited biofilm formation, the expression of α-hemolysin, the hemolytic activity, and the production of proteases and lipases of S. aureus. When applied to infected wounds in Kunming mice, juglone (50 μL juglone with a concentration of 1000 μg/mL) significantly inhibited the number of S. aureus and had a significant inhibitory effect on the expression of inflammatory mediators (TNF-α, IL-6 and IL-1β). Moreover, the juglone-treated group promoted wound healing. At the same time, in animal toxicity experiments, juglone had no obvious toxic effects on the main tissues and organs of mice, indicating that juglone has good biocompatibility and has the potential to be used in the treatment of wounds infected with S. aureus.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Antibacterial Activity of Juglone Revealed in a Wound Model of Staphylococcus aureus Infection

Wan

Wang

Liu

et al. 2023

IJMS

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“…coli), Staphylococcus aureus (S. aureus), and even the drug-resistant bacteria. − Moreover, wound infections induced by multi-drug resistant bacteria and the uncontrollable and potential cytotoxic nature of antibiotics have become important threats to wound healing . So, it is an urgent need to develop safe antimicrobial strategies to inhibit bacterial growth at the wound site, especially the drug-resistant bacteria.…”

Section: Introductionmentioning

confidence: 99%

Photocatalytic Ag/AgBr-MBG for Rapid Antibacterial and Wound Repair

Chen

Bai

et al. 2023

ACS Biomater. Sci. Eng.

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In daily life and during surgery, the skin, as the outermost organ of the human body, is easily damaged to form wounds. If the wound was infected by the bacteria, especially the drug-resistant bacteria such as methicillin-resistant staphylococcus aureus (MRSA), it was difficult to recover. Therefore, it was important to develop the safe antimicrobial strategy to inhibit bacterial growth in the wound site, in particular, to overcome the problem of bacterial drug resistance. Here, the Ag/AgBr-loaded mesoporous bioactive glass (Ag/AgBr-MBG) was prepared, which had excellent photocatalytic properties under simulated daylight for rapid antibacterial activity within 15 min by generating reactive oxygen species (ROS). Meanwhile, the killing rate of Ag/AgBr-MBG against MRSA was 99.19% within 15 min, which further reduced the generation of drug-resistant bacteria. In addition, Ag/ AgBr-MBG particles could disrupt bacterial cell membranes, showing the broad-spectrum antibacterial properties and promoting tissue regeneration and infected wound healing. Ag/AgBr-MBG particles might have potential applications as a light-driven antimicrobial agent in the field of biomaterials.

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Cratylia mollis lectin reduces inflammatory burden induced by multidrug-resistant Staphylococcus aureus in diabetic wounds

dos Santos Silva,

dos Santos Castelo Branco,

Silva

et al. 2024

Histochem Cell Biol

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Immunomodulatory and anti-infective effects of Cratylia mollis lectin (Cramoll) in a model of wound infection induced by Staphylococcus aureus

Cited by 13 publications

References 51 publications

Antibacterial Activity of Juglone Revealed in a Wound Model of Staphylococcus aureus Infection

Antibacterial Activity of Juglone Revealed in a Wound Model of Staphylococcus aureus Infection

Photocatalytic Ag/AgBr-MBG for Rapid Antibacterial and Wound Repair

Cratylia mollis lectin reduces inflammatory burden induced by multidrug-resistant Staphylococcus aureus in diabetic wounds

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