Immunomodulatory and antioxidant effects of Astragalus polysaccharide liposome in large yellow croaker (Larimichthys crocea)

Zhang, Weini; Zhang, Mengxin; Cheng, Anyi; Hao, Entian; Huang, Xiaohong; Chen, Xinhua

doi:10.1016/j.fsi.2020.03.004

Cited by 48 publications

(15 citation statements)

References 46 publications

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“…The operational approach referred to Zuo et al (24). The primer sequences for proliferating cell nuclear antigen (pcna), tight zonula occludens-1 (zo-1), tight zonula occludens-2 (zo-2), occludin, ornithine decarboxylase (odc), interleukin-1b (il-1b), interleukin-6 (il-6), interleukin-8 (il-8), interferon g (ifng), cyclooxygenase-2 (cox-2) and b-actin were designed and synthesized based on the corresponding sequences in GenBank and published papers ( Supplementary Table 2) (20,25,26). The fluorescence data acquired during the extension phase were normalized to b-actin via 2 −DDCT methods (27).…”

Section: Cdna Synthesis and Real-time Quantitative Polymerase Chain Rmentioning

confidence: 99%

Early Life Intervention Using Probiotic Clostridium butyricum Improves Intestinal Development, Immune Response, and Gut Microbiota in Large Yellow Croaker (Larimichthys crocea) Larvae

Yin

Liu

et al. 2021

Front. Immunol.

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Marine fish larvae are vulnerable during the early life period. The early intervention using probiotics may be a promising method to improve growth of fish larvae. In this study, a 30-day feeding trial was conducted to evaluate the effects of early life intervention using probiotic Clostridium butyricum (CB) on growth performance, intestinal development, immune response and gut microbiota of large yellow croaker (Larimichthys crocea) larvae. Four isonitrogenous and isolipidic diets were formulated with the supplementation of four different levels of CB (5 × 109 CFU g−1), 0.00% (Control), 0.10% (CB1), 0.20% (CB2), and 0.40% (CB3). Results showed that larvae fed diets with CB had significant higher final length than the control group. Meanwhile, larvae fed the diet with 0.10% CB had significant higher final weight and specific growth rate (SGR) than the control group. However, no significant difference in survival rate was observed among dietary treatments. CB supplementation significantly increased the height of intestinal villus and the length of intestinal enterocyte. Similarly, CB supplementation significantly increased the expression of tight zonula occludens-2 (zo-2) and ornithine decarboxylase (odc) than the control group. Larvae fed the diet with 0.20% CB had significant higher lipase and leucine-aminopeptidase (LAP) activity than the control group. Moreover, CB supplementation significantly improved immune enzyme activities than the control group. Sequencing of bacterial 16S rRNA V4-5 region indicated that dietary CB altered intestinal microbiota profile and decreased intestinal microbial diversities of larvae. CB supplementation could effectively increase the abundance of CB, and decrease the abundance of some potential pathogenic bacteria in larval gut. These results revealed that early life intervention using 0.10–0.20% CB could promote growth of large yellow croaker larvae probably through promoting intestinal development, improving immune enzyme activities and modulating gut microbiota.

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Section: Cdna Synthesis and Real-time Quantitative Polymerase Chain Rmentioning

confidence: 99%

Early Life Intervention Using Probiotic Clostridium butyricum Improves Intestinal Development, Immune Response, and Gut Microbiota in Large Yellow Croaker (Larimichthys crocea) Larvae

Yin

Liu

et al. 2021

Front. Immunol.

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“…In a similar sense, Jia et al reported that dietary supplementation with AS (1.5 and 3 g/kg) considerably increased the reduced serum TP and ALB values in CCl 4 -exposed common carps ( 31 ). Also, dietary AS increased serum TP and ALB in large yellow croakers ( 59 ). Increased serum proteins are closely related to the enhanced immunity by AS feeding.…”

Section: Discussionmentioning

confidence: 98%

“…Increased serum LYZ activity was also noticed in sea cucumber-based diets supplemented with AS or AM ( 25 ). Furthermore, dietary AS enhanced NO production, increased the phagocytic activity of head kidney macrophages and serum LYZ activities in large yellow croakers ( 59 ).…”

Section: Discussionmentioning

confidence: 99%

Immunosuppressive Effects of Thallium Toxicity in Nile Tilapia Fingerlings: Elucidating the Rescue Role of Astragalus membranaceus Polysaccharides

et al. 2022

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This study evaluated the immunotoxic effects of thallium (Tl) in Nile tilapia fingerlings and the recovery role of dietary Astragalus membranaceus polysaccharides (ASs). An 8-week experiment was designed where 180 fishes were randomly and equally assigned in triplicates into the six groups: the control group (CNT) was reared in unpolluted water and fed a commercial diet, two groups were fed a well-balanced commercial diet plus 1.5 and 3.0 g AS/kg diet (AS0.15 and AS0.30), respectively, the fourth group was exposed to a sublethal dose of Tl (41.9 μg l−1) [equal to 1/10 of 96-h lethal concentration 50 (LC50)], and the last two groups were fed 0.15 and 0.3% AS, respectively, and concurrently exposed to Tl (41.9 μg l−1) (AS0.15+Tl and AS0.30+Tl). Fish hematobiochemical parameters, serum immunity [nitric oxide, total immunoglobulin M (IgM) levels, and lysozyme activity], transcription of hepatic interferon-γ (IFN-γ), interleukin-1β (IL-1β), and tumor necrosis factor-α (TNF-α), and resistance to Aeromonas hydrophila (A. hydrophila) were assessed. Hematobiochemical parameters and serum immune indices were significantly decreased in the fish group exposed to sublethal Tl concentration compared to the CNT group. Furthermore, Tl exposure significantly induced overexpression of IL-1β, TNF-α, and IFN-γ genes (4.22-, 5.45-, and 4.57-fold higher, respectively) compared to CNT values. Tl exposure also increased the cumulative mortality (%) in Nile tilapia challenged with A. hydrophila. Remarkably, the groups fed AS0.15+Tl and AS0.30+Tl significantly ameliorated all the aforementioned parameters, but did not reach CNT values. Our findings suggest the possible immunomodulating roles of dietary AS in recovering the immunotoxic effects of Tl in Nile tilapia. We can conclude that dietary AS would be useful for maintaining the immunity of Nile tilapia fingerlings.

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“…Many studies have shown that Astragalus extract has biological functions such as improving immune function, anti-oxidation, anti-stress, anti-virus, improving intestinal structure, and promoting food and growth [39]. Oral consumption of A. hamosus root extract has been widely prescribed in traditional medicine system [40].…”

Section: Discussionmentioning

confidence: 99%

The effectiveness of the Astragalus root phenolic extracts on mice blood profile

AbdulSatar

Al-Shahwany

Ismaeel

2022

ijhs

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The aim of this study was to determine the effectiveness of Astragalus hamosus root phenolic extracts (APE) on blood profiles in albumin mice. Twenty male mice were divided into 4 groups, each with five mice, that were treated orally with a plant extract in three doses (0.3,0.5 and 1.3 mg.ml-1). The chemical compositions of (APE) were analyzed using Gas chromatography-mass spectrometry (GC–MS). In contrast to the results, Thymol a was the main active volatile monoterpenoid phenol ingredient in the phenolic compounds. Also, the results showed that the dose of 1.3 mg.ml-1 of (APE) was the powerful extract that reduced levels of cholesterol (CHO), low-density lipoprotein (LDL), very-low-density lipoprotein (VLDL), and triglycerides (TG), respectively, compared with the control treatment. Besides, the effective extract increased high-density lipoprotein (HDL) level with high significant differences compared with the control in serum mice blood. Also, the dose 1.3 mg.ml-1 increased the Monocyte count, granulocyte count, Hemoglobin with no changing in RBC, WBC level compared to the control treatment. In addition, of (APE) reduce Platelet count and Lymphocyte in the blood of the mice after four weeks.

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Immunomodulatory and antioxidant effects of Astragalus polysaccharide liposome in large yellow croaker (Larimichthys crocea)

Cited by 48 publications

References 46 publications

Early Life Intervention Using Probiotic Clostridium butyricum Improves Intestinal Development, Immune Response, and Gut Microbiota in Large Yellow Croaker (Larimichthys crocea) Larvae

Early Life Intervention Using Probiotic Clostridium butyricum Improves Intestinal Development, Immune Response, and Gut Microbiota in Large Yellow Croaker (Larimichthys crocea) Larvae

Immunosuppressive Effects of Thallium Toxicity in Nile Tilapia Fingerlings: Elucidating the Rescue Role of Astragalus membranaceus Polysaccharides

The effectiveness of the Astragalus root phenolic extracts on mice blood profile

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