Immunomodulatory effect of Artemisia argyi polysaccharide on peripheral blood leucocyte of broiler chickens

Abstract

This study was conducted to investigate the immunomodulatory effect of a water-soluble polysaccharide extracted from Artemisia argyi (AAP) in vitro. The effect was assessed in peripheral blood leucocytes (PBLs) of broilers, which were incubated with different AAP concentrations (0, 25, 50, 100, and 200 μg/ml) for 24 hr at 37°C in a 5% CO incubator. The results showed that, compared with the control group, immunoglobulin M (IgM) concentration was increased in the supernatant of the 100 μg/ml AAP-treated group (p < .05), and immunoglobulin G (IgG) concentration was increased in the supernatant of the 200 μg/ml of AAP group (p < .05). In terms of cytokine production, production of interleukin-1beta (IL-1β), interleukin-6 (IL-6) and tumour necrosis factor-alpha (TNF-α) in the supernatant was enhanced in the AAP group in a dose-dependent function, as well as enhanced mRNA expressions were showed in the cells (p < .05). The highest concentration of these three cytokines was observed in different AAP groups (IL-1β for 25 μg/ml of AAP, IL-6 for 100, and 200 μg/ml of AAP, and TNF-α for 100 μg/ml of AAP respectively). The concentration of nitric oxide (NO) was increased when using AAP at the concentration of 100 μg/ml (p < .05) as compared to the control group. No significant effects on inducible nitric oxide synthase, Toll-like receptor 4 (TLR4), myeloid differentiation factor 88 and nuclear factor Kappa B (NF-κB) mRNA level were observed at each concentration of AAP. In conclusion, we found that AAP can specifically promote the production of immunoglobulins (IgM and IgG), cytokines (IL-1β, IL-6 and TNF-α), as well as the NO concentration in vitro, but not through the activation of the TLR4/NF-κB signalling pathway.

A novel polysaccharide isolated from Flammulina velutipes, characterization, macrophage immunomodulatory activities and its impact on gut microbiota in rats

“…The extraction method of crude polysaccharide from F. velutipes was in compliance with our previous literature (Zhang, Shi, et al, ). Crude polysaccharides (100 mg) was weighted and dissolved in 10 ml of distilled water.…”

“…The extraction method of crude polysaccharide from F. velutipes was in compliance with our previous literature (Zhang, Shi, et al, 2018).…”

Water extract of Artemisia ordosica enhances antioxidant capability and immune response without affecting growth performance in weanling piglets

“…Among the bio‐active components, methoxyflavones which belong to flavonoids have been reported to be involved in preventing against oxidative damage and inflammation (Li, Lo, & Ho, ; Wang et al, ). Furthermore, total polysaccharides were recently extracted from A. argyi and their immunomodulatory activity was confirmed (Zhang et al, ). Previous study reported that the antioxidant protection of apple, similar to that of many other dietary plants, may be due largely to the presence of phenolics (Eberhardt, Lee, & Liu, ; Tsao, Yang, JCY, & Zhu, ).…”

“…Artemisia species are one of the most popular plants in Chinese traditional herbal medicine and are frequently used for the treatment of diseases such as malaria, hepatitis, cancer, inflammation and infections caused by fungi, bacteria and viruses (Wright, ). Owing to their rich nutrients and bio‐active components, the genus Artemisia plants and their extracts can be used as natural Chinese herbal medicine feed additive, which plays important roles in promoting animal growth performance, improving the quality of animal products and enhancing the immune function and antioxidant activity (Bhakuni, Jain, Sharma, & Kumar, ; Brisibe, Umoren, Owai, & Brisibe, ; Gouveia & Castilho, ; Kim, Adesogan, & Shin, ; Kim et al, ; Li et al, ; Sani, Mohammadi, Sendi, Abolghasemi, & Mehr, ; zhang et al, , ). Among genus Artemisia plants, Artemisia ordosica , a perennial herb, is one of the main shrubs growing in north and northwest areas in China.…”

“…Other properties of the plant include accelerating plant community succession, protecting eco‐environment, improving environmental conditions and fixing sand dune in desert region (Yang et al, ). Moreover, Artemisia ordosica is rich in polysaccharides, flavonoids, organic acids and other bio‐active compositions (zhang et al, ). Immunomodulatory activity of water extracts from the air‐dried acrial parts of Artemisia ordosica has been demonstrated (Li et al, ).…”