Immunomodulatory Effect of Propolis Extract on Population of IL-10 and TGF-β Expression in CD4+CD25+ Regulatory T Cells in DMBA-induced Breast Cancer in Female Sprague-Dawley Rats

Kusnul, Zauhani; Rahayu, Pudji; Rifa’i, Muhaimin; Widjajanto, Edi

doi:10.25002/tji.2017.573

Cited by 5 publications

(6 citation statements)

References 9 publications

(11 reference statements)

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“…There is also evidence that propolis affects immune response in the presence of breast cancers. 66 Kusnul et al 96 reported that propolis significantly reduced the relative number of CD4+, CD25+, FoxP3+ regulatory T cells expressing IL-10 and TGF-β in mice with breast cancer. The suppression of IL-10, which is an immunosuppressive cytokine, is thought to be beneficial in treating cancers.…”

Section: Cytotoxicity Against Breast Cancer Cellsmentioning

confidence: 99%

The Potential Use of Propolis as an Adjunctive Therapy in Breast Cancers

et al. 2022

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Propolis is a resinous beehive product that has a wide range of biological activities, namely antimicrobial, antioxidant, and anti-inflammatory properties. Propolis is collected by the bees from plant resin and exudates to protect hives and maintain hive homeostasis. The aim of the present systematic scoping review is to explore the potential and suitability of propolis as an adjunctive treatment in breast cancers, based on the latest available experimental evidence (2012-2021). After applying the exclusion criteria, a total of 83 research publications were identified and retrieved from Scopus, Web of Science, and Pubmed. Several relevant key themes identified from the included studies were cytotoxicity, synergistic/combination treatment, improvement in bioavailability, human clinical trials, and others. A majority of the studies identified were still in the in vitro and in vivo stages. Nonetheless, we managed to identify 4 human clinical trials that demonstrated the successful use of propolis in alleviating side effects of chemotherapy and radiotherapy while increasing the quality of life of breast cancer patients, with minimal adverse effects. In conclusion, propolis, as an adjunctive treatment, may have therapeutic benefits in alleviating symptoms related to breast cancers. However, further clinical trials, preferably with higher number of participants/subjects/patients, are urgently needed.

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Section: Cytotoxicity Against Breast Cancer Cellsmentioning

confidence: 99%

The Potential Use of Propolis as an Adjunctive Therapy in Breast Cancers

et al. 2022

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“…8 Secara in vivo ekstrak propolis juga telah dibuktikan mampu meningkatkan respon imun anti kanker pada tikus Spraque-Dawley yang diinduksi mengalami kanker payudara dengan menggunakan DMBA. 10 Selain itu keamanan penggunaan ekstrak propolis terhadap organ ginjal dan hepar juga telah diuji dengan uji toksisitas subkronik pada tikus wistar dan hasilnya menunjukkan bahwa ekstrak propolis aman untuk dikonsumsi (data belum dipublikasi). nyata.…”

Section: Pendahuluanunclassified

Ekstrak Propolis Memperbaiki Profil Berat Badan Tikus Model Kanker Payudara yang Diinduksi dengan 7,12-dymethyilbenz(a) antracene (DMBA)

Kusnul¹,

Suryono²,

Tamsuri³

2019

mpk

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Body weight is a general indicator for assessing health status. Various diseases cause drastic weight loss, including cancer. Propolis is a bee product that has various therapeutic effects such as; anti-bacterial, antitumor, antioxidant and immunomodulatory. Propolis is also reported to be able to reduce digestive organ disorders, increase appetite and improve metabolic processes. Chemicals such as 7.12-dymethyilbenz (a) antracene (DMBA) are widely reported to have strong carcinogenic effects, especially in Sprague–Dawley rat. This study aims to assess the effect of propolis extract on the weight of Sprague–Dawley female rat induced with DMBA (7,12-dymethylbenz (a) antracene). Twenty-four female Sprague–Dawley rats 45-50 days old were induced by DMBA with a combination of injection and oral methods, as negative controls 6 Sprague–Dawley rats without DMBA induction. At the 11th week randomized negative control rats and DMBA treated rats were taken for histopathological examination of breast tissue. After it was found that rat with DMBA treatment were positive for breast cancer, in the 12th week the rat that had received DMBA treatment were divided into 4 groups, 3 groups received oral propolis extract through a gastric sonde with doses 50, 100 and 200 mg in 1 ml of corn oil, 1 group as a positive control did not get the treatment of propolis extract. Body weight is weighed before starting treatment and monitored every two weeks to 15 weeks. The results of weighing showed that the group of rat that received DMBA increased their body weight lower than the group without DMBA, and then the treatment group of propolis extract increased their body weight higher than the group without the treatment of propolis extract. The results showed that the treatment of propolis extract had a potency to improve the body weight profile of rat breast cancer model induced by DMBA. Abstrak Berat badan merupakan indikator umum untuk menilai status kesehatan. Berbagai penyakit menyebabkan penurunan berat badan yang drastis, diantaranya adalah kanker. Propolis merupakan produk lebah yang memiliki berbagai efek terapi seperti; anti bakteri, anti tumor, antioksidan dan imunomodulator. Propolis juga dilaporkan mampu menurunkan gangguan organ pencernaan, peningkatan nafsu makan, dan perbaikan proses metabolisme. Bahan kimia seperti 7,12-dymethyilbenz(a)antracene (DMBA) banyak dilaporkan memiliki efek karsinogenik yang kuat khususnya terhadap tikus Sprague–Dawley. Penelitian ini bertujuan untuk menilai pengaruh pemberian ekstrak propolis terhadap berat badan tikus Sprague–Dawley betina yang diinduksi untuk mengalami kanker payudara dengan DMBA. Sebanyak 24 ekor tikus Sprague– Dawley betina berumur 45-50 hari diinduksi dengan DMBA dengan kombinasi metode injeksi dan oral, sebagai kontrol negatif 6 ekor tikus Sprague–Dawley tanpa induksi DMBA. Pada minggu ke-11 diambil secara acak tikus kontrol negatif dan tikus perlakuan DMBA untuk dilakukan pemeriksaan histolopatogi jaringan payudara. Setelah didapatkan bahwa tikus dengan perlakuan DMBA positif mengalami kanker payudara, pada minggu ke-12 tikus yang telah mendapat perlakuan DMBA dibagi menjadi 4 kelompok, 3 kelompok mendapat ekstrak propolis oral melalui sonde dengan dosis masing-masing 50, 100, dan 200 mg dalam 1 ml minyak jagung, 1 kelompok sebagai kontrol positif tidak mendapat perlakuan ekstrak propolis. Berat badan ditimbang sebelum mulai perlakuan dan dipantau tiap dua minggu sampai 15 minggu. Hasil penimbangan berat badan menunjukkan bahwa kelompok tikus yang mendapat DMBA peningkatan berat badannya lebih rendah dibanding kelompok tanpa DMBA, dan selanjutnya kelompok perlakuan ekstrak propolis kenaikan berat badannya lebih tinggi dibanding kelompok tanpa perlakuan ekstrak propolis. Hasil penelitian menunjukkan bahwa perlakuan ekstrak propolis memiliki potensi memperbaiki profil berat badan tikus model kanker payudara yang diinduksi dengan DMBA.

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“…Dalam dunia pengobatan, propolis sudah digunakan sejak dahulu kala dan hingga kini telah diketahui propolis bisa menjadi obat alami dengan kemampuan anti bakteri (Hasson, 2014), anti tumor (Borges et al, 2011;Khacha-ananda et al, 2013), antioksidan (Padmavathi et al, 2006), dan imunomodulator (Sforcin, Kaneno dan Funari, 2011;Kusnul et al, 2017).…”

Section: Pendahuluanunclassified

PREDIKSI INTERAKSI MOLEKUER CAPE DENGAN IL-2, CD25, IL-10, CTLA-4, IDO, TGFβ, and CCL2 DENGAN SOFTWARE DOCKING MOLEKULER

Kusnul

2019

SAINTEKBU

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The use of herbal medicine as a nutritious ingredient to treat various diseases is still mostly done traditionally based on hereditary information. The development of technology supports the exploration of natural materials as a nutritious material with various scientific evidences. The development of computational and bioinfrmatic technology simulates interactions between various active compounds and target molecules virtually commonly known as molecular docking, a virtual screening based on structure used to predict the interaction of three-dimensional structures of small molecules (compounds) with the target molecular structure. Propolis is a substance produced by bees from resins collected from plants and combined with wax and secretions from bee salivary glands which are rich in various enzymes. In the world of medicine, propolis has been used since time immemorial and until now it has been known that propolis can be a natural medicine with anti-bacterial, anti-tumor, antioxidant and immunomodulatory abilities. Molecular docking between one of the active compounds in propolis, namely caffeic acid phenethyl ester (CAPE) with various target molecules such as IL-2, CD25, IL10, TGFβ, CTLA-4, CCL21, and IDO using the DockingServer software provides information that CAPE has inhibitory activity against IL-2, CD25, IL-10, CTLA-4, IDO, TGFβ, and CCL2 so that it has the potential to be further developed as a candidate for immunomodulatory therapy.

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Immunomodulatory Effect of Propolis Extract on Population of IL-10 and TGF-β Expression in CD4+CD25+ Regulatory T Cells in DMBA-induced Breast Cancer in Female Sprague-Dawley Rats

Abstract: Introduction: The aim of this study was to investigate the effect of propolis extract to the population of regulatory T cells (CD4 + CD25 + FoxP3 + ) expressing IL-10 and TGF-β in vivo (DMBA-induced breast cancer in Female SpragueDawley Rats).

Cited by 5 publications

References 9 publications

The Potential Use of Propolis as an Adjunctive Therapy in Breast Cancers

The Potential Use of Propolis as an Adjunctive Therapy in Breast Cancers

Ekstrak Propolis Memperbaiki Profil Berat Badan Tikus Model Kanker Payudara yang Diinduksi dengan 7,12-dymethyilbenz(a) antracene (DMBA)

PREDIKSI INTERAKSI MOLEKUER CAPE DENGAN IL-2, CD25, IL-10, CTLA-4, IDO, TGFβ, and CCL2 DENGAN SOFTWARE DOCKING MOLEKULER

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