Immunomodulatory effect of short-term supplementation with Bacillus toyonensis BCT-7112T and Saccharomyces boulardii CNCM I-745 in sheep vaccinated with Clostridium chauvoei

Santos, Francisco Denis Souza; Maubrigades, Lucas Reichert; Gonçalves, Vitória Sequeira; Ferreira, Marcos Roberto Alves; Brasil, Carolina Litchina; Cunha, Rodrigo Casquero; Conceição, Fabrício Rochedo

doi:10.1016/j.vetimm.2021.110272

Cited by 12 publications

(10 citation statements)

References 52 publications

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“…Likewise, the Lactococcus lactis strain decreased severity and symptoms in volunteers with Dengue fever (DF) compared to the placebo group, promoted IFN-γ and TGF-β cytokines secretion, and reduced serum IgE and IL-4 cytokine levels in mice models (111,112). Bacillus toyonensis (B. toyonensis) BCT-7112 was also enabled to improve the humoral immune response of ewes against the clostridium perfringens epsilon toxin (rETX) vaccine and boost higher neutralizing antibody titers (113). B. toyonensis and Saccharomyces boulardii also successfully boosted antibody production and expression of IFN-γ, IL2, and Bcl6 genes in Clostridium chauvoei vaccinated sheep (114).…”

Section: The E Ects Of Probiotics On Vaccine E Cacymentioning

confidence: 99%

“…toyonensis ) BCT-7112 was also enabled to improve the humoral immune response of ewes against the clostridium perfringens epsilon toxin (rETX) vaccine and boost higher neutralizing antibody titers ( 113 ). B. toyonensis and Saccharomyces boulardii also successfully boosted antibody production and expression of IFN-γ, IL2, and Bcl6 genes in Clostridium chauvoei vaccinated sheep ( 114 ). Likewise, Bacillus velezensis significantly reduced the pigeon circovirus (PiCV) viral load in the feces and spleen of pigeons and promoted TLR 2&4 expression ( 115 ).…”

Section: The Effects Of Probiotics On Vaccine Efficacymentioning

confidence: 99%

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Probiotics and probiotic-based vaccines: A novel approach for improving vaccine efficacy

2022

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Vaccination is defined as the stimulation and development of the adaptive immune system by administering specific antigens. Vaccines' efficacy, in inducing immunity, varies in different societies due to economic, social, and biological conditions. One of the influential biological factors is gut microbiota. Cross-talks between gut bacteria and the host immune system are initiated at birth during microbial colonization and directly control the immune responses and protection against pathogen colonization. Imbalances in the gut microbiota composition, termed dysbiosis, can trigger several immune disorders through the activity of the adaptive immune system and impair the adequate response to the vaccination. The bacteria used in probiotics are often members of the gut microbiota, which have health benefits for the host. Probiotics are generally consumed as a component of fermented foods, affect both innate and acquired immune systems, and decrease infections. This review aimed to discuss the gut microbiota's role in regulating immune responses to vaccination and how probiotics can help induce immune responses against pathogens. Finally, probiotic-based oral vaccines and their efficacy have been discussed.

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Section: The E Ects Of Probiotics On Vaccine E Cacymentioning

confidence: 99%

Section: The Effects Of Probiotics On Vaccine Efficacymentioning

confidence: 99%

Probiotics and probiotic-based vaccines: A novel approach for improving vaccine efficacy

2022

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“…IL2 promotes growth and development of peripheral immune cells, initiating a defensive immune response through survival and division of regulatory T cells (Treg) and proliferation of cytotoxic T cells [32]. Santos et al [33] also reported high levels of IL2 mRNA transcription by PBMCs (Human Peripheral Blood Mononuclear Cells) from animals supplemented with S. boulardii.…”

Section: Yeasts Interact With Raw Macrophages In Different Waysmentioning

confidence: 99%

The beneficial role ofCandida intermediaandSaccharomyces boulardiiyeasts on the immune response of mice vaccinated with a SARS-CoV-2 experimental vaccine

Piraine

Conrad

Gonçalves

et al. 2021

Preprint

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Yeasts are a group of microorganisms with structural and metabolic characteristics that influence their recognition by immune cells resulting in a species-specific response. Although Saccharomyces boulardii is a widely studied probiotic yeast, immunostimulation by non-Saccharomyces yeasts still underexplored. Therefore, the aim of this study was to characterize the response induced in macrophages stimulated by yeasts Pichia kluyveri, Hanseniaspora uvarum, Candida intermedia and their derivatives: heat-killed cells, supernatant and DNA. RAW 264.7 murine macrophages were stimulated in vitro for 24 h and the response generated was evaluated by analyzing mRNA transcription of cytokines (IL2, IL4, IL10, IL13, IL23, TNF-alpha), transcription factors (Bcl6, NFkB;, STAT3), Toll-like receptor 2 (TLR2) and YM1 protein. Viable and heat-killed cells of P. kluyveri and H. uvarum were responsible for high levels of relative mRNA transcription of transcription factors and TLR2 (between 2-8-fold increase), however were able to induce only low transcription levels for analyzed cytokines (approximately 2-fold increase). Viable cells of C. intermedia were able to stimulate a significant transcription of IL4 (7.6-fold increase) and Bcl6 (4-fold increase), while heat-killed cells stimulated the highest level of TNF-alpha (2.4-fold increase) among yeasts and their derivatives. Furthermore, supernatant from C. intermedia culture induced significant (p < 0.05) levels of TLR2 (4.4-fold increase), being the only one among supernatants to present high levels of relative mRNA transcription of TLR2. Data found in this work arouse interest in further studies on interaction between non-Saccharomyces yeasts and immune system cells, mainly referring to immunomodulatory capacity.

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“…6,7 This microorganism acts by modulating the systemic and local levels of immunoglobulins, cytokines and immune cells. 8,9 The aim of this study was to evaluate the immune response of sheep supplemented with S. boulardii against infection with the nematode H. contortus.…”

Section: Introductionmentioning

confidence: 99%

Sheep immune‐stimulated with Saccharomyces boulardii show reduced prolificacy of Haemonchus contortus

Pinheiro

Gaspar

Minho

et al. 2022

Parasite Immunology

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Haemonchus contortus is the most pathogenic parasite for sheep. The objective was to evaluate immunomodulation of the probiotic Saccharomyces boulardii in sheep experimentally infected with H. contortus. Twenty-four sheep were divided into three groups: one infected with 500 H. contortus larvae/day for 26 days and supplemented with S. boulardii (40 ml with 1 Â 10 8 CFU/ml/day); a control group only infected with H. contortus but not supplemented; and a naïve group that never came into contact with either parasites or S. boulardii. To assess the humoral immune response, production of specific serum IgG anti-somatic H. contortus antigen was evaluated through indirect ELISA. To assess the cellular immune response, cell populations and cytokine (IL-4, IL-5 and IL-10) production were evaluated through flow cytometry. For parasitological analyses, the counts of eggs per gram of faeces (EPG) and larvae per faecal culture were assessed. At all the study points, the concentration of IgG anti-H. contortus was higher (p < .05) in the S. boulardii group than in the other groups. The cell analysis revealed that there were significantly higher numbers (p < .05) of cells expressing MHC-II and significantly higher numbers (p < .05) of eosinophils in the mucosa in the S. boulardii group.Significant expression of IL-10 was observed only in the control infected group. There were significant reductions (p < .05) in EPG and larval counts in the S. boulardii supplemented group. These results show that S. boulardii supplementation modulated the immune response against H. contortus, thereby reducing its infection.

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Immunomodulatory effect of short-term supplementation with Bacillus toyonensis BCT-7112T and Saccharomyces boulardii CNCM I-745 in sheep vaccinated with Clostridium chauvoei

Cited by 12 publications

References 52 publications

Probiotics and probiotic-based vaccines: A novel approach for improving vaccine efficacy

Probiotics and probiotic-based vaccines: A novel approach for improving vaccine efficacy

The beneficial role ofCandida intermediaandSaccharomyces boulardiiyeasts on the immune response of mice vaccinated with a SARS-CoV-2 experimental vaccine

Sheep immune‐stimulated with Saccharomyces boulardii show reduced prolificacy of Haemonchus contortus

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