Immunomodulatory Effect of Tremella Polysaccharides against Cyclophosphamide-Induced Immunosuppression in Mice

Abstract

Polysaccharides are closely associated with immune regulation, but there are different polysaccharide effects from different sources. In this study, the aim was to investigate the effect of tremella polysaccharides (TP) in cyclophosphamide-induced immunodeficient mice. We observed the thymus and spleen index, liver and spleen pathological changes, and the levels of IL-2, IL-12, INF-γ, TGF-β and Ig G in serum, and we also noted the mRNA expression of IL-1β, IL-4, IL-12 and TGF-β in liver and spleen. Besides, we also measured the best effects of different doses of TP (Low-TP was 20 mg/kg·BW, Middle-TP was 40 mg/kg·BW, and High-TP was 80 mg/kg·BW) on cyclophosphamide-induced immunosuppressed mice. The results were remarkable, and suggested that TP had a significant effect for enhancing immunity in cyclophosphamide-induced immunosuppression, and the immune enhancement of High-TP had the best results in TP-treated mice. It could significantly increase the thymus and spleen index, alleviate pathological features of immunosuppression such as the arrangement of liver sinusoid and hepatic plates was disordered, massive inflammatory cells infiltrated and fatty degeneration of hepatocytes in liver, and red pulp and white pulp were intermixed, splenic corpuscles demolished and disappeared, splenic sinusoid extended, and lymphocytes of spleen were reduced in spleen. Besides, it could also up-regulate serum levels of IL-2, IL-12, INF-γ and Ig G, reduce the level of TGF-β in serum, markedly promote mRNA expression of IL-1β, IL-4 and IL-12 in liver and spleen, and suppress mRNA expression of TGF-β. Above all, TP showed preventive effect for cyclophosphamide-induced immunosuppressed mice.

Effect of Tea Polyphenol on Serum Hormone, Serum Enzyme Activity and Antioxidant-Related Gene Expression in Chinese Yellow Chicken under Heat Stress

“…It not only activates macrophages but also activates NK cells [54]. And it is a highly potent antiviral cytokine with broad-spectrum immunomodulatory effects [55]. It is reported that the beginning of IL-2 function depends on B and T lymphocytes, which is one of the most important lymphoid factors regulating the immune function of the body [56].…”

Influence of drying methods on the physicochemical properties and nutritional composition of instant Tremella fuciformis

“…T. fuciformis is rich in active polysaccharides (Wu et al, 2019). On recent years, many reports had shown that T. fuciformis polysaccharides possessed various bioactivities (Wu et al, 2019), including antioxidation, anti-tumor (Chen, 2010), anti-aging (Wen et al, 2016), immunomodulatory (Zhou et al, 2018), and anti-inflammator (Lee et al, 2016;Yang et al, 2018).…”

Immunostimulatory Effect of Zanthoxylum schinifolium‐Based Complex Oil Prepared by Supercritical Fluid Extraction in Splenocytes and Cyclophosphamide‐Induced Immunosuppressed Rats

“…Administration of Cy can lead to a sudden change in Th1/Th2 bias, resulting in immunosuppression [ 19 ]. In previous studies, the immunological effects of Cy were found to decrease the absolute number of T cells, proliferation of lymphocytes, Th1 cell produced cytokines (IL-2, IL-12, and IFN- γ ), and Th2 produced cytokine (IL-4) [ 20 ].…”