Immunomodulatory Effects of Adipose-Derived Stem Cells: Fact or Fiction?

Barone, Angelo A. Leto; Khalifian, Saami; Lee, W. P. Andrew; Brandacher, Gerald

doi:10.1155/2013/383685

Cited by 96 publications

(86 citation statements)

References 81 publications

(128 reference statements)

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“…Several recognized clinical studies have thus been performed to investigate the potential of BMSCs for the treatment of GVHD [15,[70][71][72][73]. In addition, both preclinical and clinical studies have been performed to evaluate the use of ASCs to prevent acute GVHD, as reviewed by Leto Barone et al [74]. Nonetheless, if ASCs are used for patients for immunomodulatory purposes, it is important to carefully assess the risk for the possibility of tumor formation connected with ASC-mediated suppression.…”

Section: Discussionmentioning

confidence: 99%

Different Culture Conditions Modulate the Immunological Properties of Adipose Stem Cells

Patrikoski

Sivula

Huhtala

et al. 2014

Stem Cells Translational Medicine

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The potential of human adipose stem cells (ASCs) for regenerative medicine has received recognition owing to their ease of isolation and their multilineage differentiation capacity. Additionally, low immunogenicity and immunosuppressive properties make them a relevant cell source when considering immunomodulation therapies and allogeneic stem cell treatments. In the current study, immunogenicity and immunosuppression of ASCs were determined through mixed lymphocyte reactions. The immunogenic response was analyzed after cell isolation and expansion in fetal bovine serum (FBS), human serum (HS)-supplemented medium, and xeno-free and serum-free (XF/SF) conditions. Additionally, the immunophenotype and the secretion of CXC chemokine ligand 8 (CXCL8), CXCL9, CXCL10, C-C chemokine ligand 2 (CCL2), CCL5, interleukin 2 (IL-2), IL-4, IL-6, IL-10, IL-17A, tumor necrosis factora, interferon-g, transforming growth factor-b1, indoleamine 2,3-deoxygenase, Galectin-1, and Galectin-3 were analyzed. The results showed that ASCs were weakly immunogenic when expanded in any of the three conditions. The significantly strongest suppression was observed with cells expanded in FBS conditions, whereas higher ASC numbers were required to display suppression in HS or XF/SF conditions. In addition, statistically significant differences in protein secretion were observed between direct versus indirect cocultures and between different culture conditions. The characteristic immunophenotype of ASCs was maintained in all conditions. However, in XF/SF conditions, a significantly lower expression of CD54 (intercellular adhesion molecule 1) and a higher expression of CD45 (lymphocyte common antigen) was observed at a low passage number. Although culture conditions have an effect on the immunogenicity, immunosuppression, and protein secretion profile of ASCs, our findings demonstrated that ASCs have low immunogenicity and promising immunosuppressive potential whether cultured in FBS, HS, or XF/SF conditions. STEM CELLS TRANSLATIONAL

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Section: Discussionmentioning

confidence: 99%

Different Culture Conditions Modulate the Immunological Properties of Adipose Stem Cells

Patrikoski

Sivula

Huhtala

et al. 2014

Stem Cells Translational Medicine

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“…The niche of ASCs in adipose tissue has not been clarified. However, a number of recent studies indicate that it can be identified in adipose tissue vascularization process like shown by (Leto Barone et al 2013; Lin et al 2008a; Zimmerlin et al 2010). Zimmerlin et al (2010) identified the ASCs on outer adventitia of blood vessels.…”

Section: Phenotype and Niche Of Ascsmentioning

confidence: 92%

Adipose-Derived Stem Cells as a Tool in Cell-Based Therapies

Bajek

Gurtowska

Olkowska

et al. 2016

Arch. Immunol. Ther. Exp.

144

108

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Recent development in stem cell isolation methods and expansion under laboratory conditions create an opportunity to use those aforementioned cells in tissue engineering and regenerative medicine. Particular attention is drawn towards mesenchymal stem cells (MSCs) being multipotent progenitors exhibiting several unique characteristics, including high proliferation potential, self-renewal abilities and multilineage differentiation into cells of mesodermal and non-mesodermal origin. High abundance of MSCs found in adipose tissue makes it a very attractive source of adult stem cells for further use in regenerative medicine applications. Despite immunomodulating properties of adipose-derived stem cells (ASCs) and a secretion of a wide variety of paracrine factors that facilitate tissue regeneration, effectiveness of stem cell therapy was not supported by the results of clinical trials. Lack of a single, universal stem cell marker, patient-to-patient variability, heterogeneity of ASC population combined with multiple widely different protocols of cell isolation and expansion hinder the ability to precisely identify and analyze biological properties of stem cells. The above issues contribute to conflicting data reported in literature. We will review the comprehensive information concerning characteristic features of ASCs. We will also review the regenerative potential and clinical application based on various clinical trials.

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“…In addition, they are available in higher quantities and exhibit more cell homogeneity. Therefore, a number of studies have investigated the effects of AMSCs in tissue regeneration and immunomodulation [7,8].…”

Section: Introductionmentioning

confidence: 99%