Immunomodulatory Effects of Hexane Insoluble Fraction of Ficus septica Burm. F. in Doxorubicin-treated Rats

Abstract

The use of chemotherapeutics induces cardiotoxicity and affects immune functions, therefore development of combinatorial agents against cardiotoxicity and immunosuppression needs to be explored. Previous studies of the hexane insoluble fraction (HIF) of an ethanolic extract of Ficus septica leaves showed anticancer effects singly and in combination with doxorubicin on T47D breast cancer cells. In this present study, it was evaluated for its immunomodulatory activities in doxorubicin-treated rats. Thirty male Sprague Dawley rats were divided into five groups consisting of six rats each as follows: Group 1, receiving oral saline 10 ml/kg BW (control group); Group 2, receiving HIF dose 750 mg/kg BW orally, once daily; Group 3, receiving HIF dose 1.500 mg/kg BW orally, once daily; Group 4, given oral saline 10 ml/kg BW (normal group); Group 5, receiving HIF dose 1.500 mg/kg BW orally, once daily. The rats of group 1-3 were intramuscularly administered with doxorubicin at a dose of 4.67 mg/kg BW at the days 1 and 4 to suppress immune functions. Concomitantly, the rats were treated with saline or HIF for seven consecutive days (1 to 7). Treatment of HIF succeeded in reducing side effects of doxorubicin based on increasing lymphocyte density and phagocytosis activity and capacity of macrophages, as well as increasing the CD8+ blood level and decreasing spleen IL-10 expression. Hexane insoluble fraction of of ethanolic extract of Ficus septica leaves has potential as a protective agent combined with doxorubicin.

Immune-modulating Effect of Korean Red Ginseng by Balancing the Ratio of Peripheral T Lymphocytes in Bile Duct or Pancreatic Cancer Patients With Adjuvant Chemotherapy

“…A decreased CD4 + /CD8 + ratio has been reported to be correlated with decreased immune function (28,29). Wang et al showed that patients with non-small cell lung cancer had a significantly lower CD4 + /CD8 + ratio than healthy controls and patients with advanced-stage cancer had a significantly lower CD4 + /CD8 + ratio than those with earlystage cancer (27).…”

Variation of Blood T Lymphocyte Subgroups in Patients with Non- small Cell Lung Cancer

“…It is suggested that CD4+/CD8+ ratio is crucial to be kept in a dynamic balance, in terms of maintaining the immunological function stable (Dou et al, 2013). the ratio decreased is reported to link with a low immunological function (Erdem et al, 2012;Nugroho et al, 2012). Our study demonstrated that patients with NSCLC had a significantly lower level of CD3+, CD4+, and CD4+/ CD8+ ratio compared with healthy controls; patients with advanced staged NSCLC had a significantly lower level of CD3+, CD4+, and CD4+/CD8+ ratio compared with those with early stage.…”

The role of chemotherapy and operation on lymphocytes accumulation in peripheral blood obtained from patients with oral squamous cell carcinoma

“…It is suggested that CD4 + /CD8 + ratio is crucial to be kept in a dynamic balance, in terms of maintaining the immunological function stable (Dou et al 2013 ). The ratio decreased is reported to link with a low immunological function (Erdem et al 2012 ; Nugroho et al 2012 ). In this study, The patients with OSCC received 2 cycles CT or radical operation had a significantly higher level of CD4 + /CD8 + ratio compared with that received no treatment or 1 cycle CT. NK cell is also designated as natural killer cells.…”