Immunomodulatory effects of Lactobacillus plantarum on human colon cancer cells

Paolillo, Rossella; Carratelli, Caterina Romano; Sorrentino, Sabato; Mazzola, Nello; Rizzo, Antonietta

doi:10.1016/j.intimp.2009.07.008

Cited by 114 publications

(80 citation statements)

References 64 publications

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“…Studies on transcriptional responses of human epithelial cells to probiotic bifidobacteria are scarce and inconclusive (35,43). More studies, however, have been performed with Lactobacillus strains, observing differences both in vitro (28,30,39) and in vivo (8,45,46) after bacterial treatment. In fact, the inducing effect on a number of genes coding for chemotactic cytokines observed in this work has also been described in in vivo studies with probiotic lactobacilli (8).…”

Section: Discussionmentioning

confidence: 99%

Interaction of Bifidobacterium bifidum LMG13195 with HT29 Cells Influences Regulatory-T-Cell-Associated Chemokine Receptor Expression

López

González-Rodríguez

Sánchez

et al. 2012

Appl Environ Microbiol

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Section: Discussionmentioning

confidence: 99%

Interaction of Bifidobacterium bifidum LMG13195 with HT29 Cells Influences Regulatory-T-Cell-Associated Chemokine Receptor Expression

López

González-Rodríguez

Sánchez

et al. 2012

Appl Environ Microbiol

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“…The consumption of Lactobacillus plantarum has been related to significant improvements in the health of humans and animals (16)(17)(18)(19). This species was the first of the genus to have its genome sequenced (20).…”

mentioning

confidence: 99%

Potential Fate of Ingested Lactobacillus plantarum and Its Occurrence in Human Feces

Costa

Marcelino-Guimarães

Vilas-Bôas

et al. 2014

Appl Environ Microbiol

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e Lactobacillus plantarum has been used in human clinical trials to promote beneficial effects in the immune system, to alleviate intestinal disorders, and to reduce the risk of cardiovascular disease. It is also involved in many fermentation processes in the food industry. However, information on the fate of ingested L. plantarum is limited. In this study, 61 subjects received daily doses of fermented milk containing 2 ؋ 10 11 cells of L. plantarum Lp115 for different periods of time. The target microorganism was monitored in the fecal microbiota via quantitative PCR (qPCR). L. plantarum was detected and quantified in all of the subjects during the ingestion periods. The differences between the L. plantarum levels at time zero and during all the different ingestion periods were statistically significant (P ‫؍‬ 0.001). However, at 15 and 45 days after discontinuing supplementation, the number of lactobacilli was reduced to the baseline level (those at time zero). A longer period with L. plantarum in the diet did not result in increased levels of this bacterium in the stool, based on postconsumption evaluations (P ‫؍‬ 0.001). The qPCR method was specific and sensitive for L. plantarum quantification in such a complex microbial environment as the gastrointestinal tract.

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“…Like other commensals [10] LB were able to attenuate inflammation mediated through TLR-2 receptor on nasopharyngeal epithelial cells (Figure 3, not all shown), as in other tissues [39]. Only L. plantarum and L. salivarious, however, were able to significantly attenuate both IL-6 and TNF-α responses (Figure 1) and none of the LB…”

Section: Inflammationmentioning

confidence: 80%

Oral <i>Lactobacillus plantarum</i> NCIMB 8825 Inhibits Adhesion, Invasion and Metabolism of <i>Neisseria meningitidis</i> Serogroup B and Affords Anti-Inflammatory and Cytotoxic Protection to Nasopharyngeal Epithelial Cells

Bekele¹,

Keith²,

Adelina³

et al. 2014

AiM

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In this study, we investigate the potential for oral Lactobacilli (LB) to afford innate protection against nasopharyngeal coloniser Neisseria meningitidis serogroup B (NmB), which causes the bulk of UK meningococcal disease. Oral isolates of L. plantarum, L. salivarious, L. casei, L. rhamnosus, L. gasseri and gut probiotic L. rhamnosus GG were assessed for their ability to suppress nasopharyngeal epithelial inflammatory responses to pathogenic NmB. The specificity of attenuation was examined using TLR 2 ligand, Pam3Cys, and early response cytokine IL1β; and the mechanism of attenuation was explored using heat-killed organisms and conditioned medium. Pro-inflammatory IL-6 and TNFα cytokine secretion was quantified by ELISA and associated cell death was quantified by PI staining and LDH release. NmB adhesion, invasion and metabolism were determined using standard gentamicin protection with viable counts, and bioluminescence, respectively. L. plantarum and L. salivarious suppressed IL-6 and TNFα secretions from NmB-infected epithelial cells. LB did not need to be alive and could suppress using secretions, which were independent of TLR2 or IL1β receptor signalling. L. plantarum, in particular, reduced NmB-induced necrotic cell death of epithelial monolayers. Like L. salivarious, it significantly inhibited NmB adhesion but uniquely L. plantarum abolished NmB invasion. Using bioluminescence as a reporter of pathogen metabolism, L. plantarum and its secretions were found to inhibit NmB metabolism during cell invasion assays. We conclude that oral L. plantarum and its secretions could be used to help reduce the burden of meningococcal disease by removing the intracellular nasopharyngeal reservoir of NmB.

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Immunomodulatory effects of Lactobacillus plantarum on human colon cancer cells

Cited by 114 publications

References 64 publications

Interaction of Bifidobacterium bifidum LMG13195 with HT29 Cells Influences Regulatory-T-Cell-Associated Chemokine Receptor Expression

Interaction of Bifidobacterium bifidum LMG13195 with HT29 Cells Influences Regulatory-T-Cell-Associated Chemokine Receptor Expression

Potential Fate of Ingested Lactobacillus plantarum and Its Occurrence in Human Feces

Oral <i>Lactobacillus plantarum</i> NCIMB 8825 Inhibits Adhesion, Invasion and Metabolism of <i>Neisseria meningitidis</i> Serogroup B and Affords Anti-Inflammatory and Cytotoxic Protection to Nasopharyngeal Epithelial Cells

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Immunomodulatory effects of Lactobacillus plantarum on human colon cancer cells

Cited by 114 publications

References 64 publications

Interaction of Bifidobacterium bifidum LMG13195 with HT29 Cells Influences Regulatory-T-Cell-Associated Chemokine Receptor Expression

Interaction of Bifidobacterium bifidum LMG13195 with HT29 Cells Influences Regulatory-T-Cell-Associated Chemokine Receptor Expression

Potential Fate of Ingested Lactobacillus plantarum and Its Occurrence in Human Feces

Oral &lt;i&gt;Lactobacillus plantarum&lt;/i&gt; NCIMB 8825 Inhibits Adhesion, Invasion and Metabolism of &lt;i&gt;Neisseria meningitidis&lt;/i&gt; Serogroup B and Affords Anti-Inflammatory and Cytotoxic Protection to Nasopharyngeal Epithelial Cells

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Oral <i>Lactobacillus plantarum</i> NCIMB 8825 Inhibits Adhesion, Invasion and Metabolism of <i>Neisseria meningitidis</i> Serogroup B and Affords Anti-Inflammatory and Cytotoxic Protection to Nasopharyngeal Epithelial Cells