Immunomodulatory effects of specific bacterial components ofLactobacillus plantarumKFCC11389P on the murine macrophage cell line RAW 264·7

Abstract

Aims: The objective of this study was to investigate the ability of specific bacterial components of Lactobacillus plantarum KFCC11389P to induce anti‐inflammatory mediators in cell cultures of the murine macrophage cell line, RAW 264·7. Methods and Results: The RAW 264·7 cells were stimulated with viable bacterial cells (VC), heat‐killed (HK) cells, cell walls (CW) or ultrafiltrates of metabolic products (UF). An increase in the levels of tumour necrosis factor (TNF)‐α was observed in VC, HK and CW, but this effect was much lower in UF. VC stimulated higher levels of interleukin (IL)‐6 releases as well as nitric oxide production than HK. In contrast, UF and its separated molecule, fraction 4, were much strong IL‐10 inducers. Fraction 4 (8·1 kDa), especially, inhibited the production of pro‐inflammatory cytokines, IL‐6 (89% decrease) and TNF‐α (55% decrease), in lipopolysaccharide (LPS)‐stimulated murine macrophages. Conclusions: The results of this study indicate that metabolic products of Lact. plantarum KFCC11389P could influence the immune‐modulating activity via IL‐10, and pretreatment with this specific molecule could inhibit LPS‐induced release of IL‐6 and TNF‐α. Significance and Impact of the Study: Our findings suggest that the specific molecules of Lact. plantarum KFCC11389P may be useful for the treatment of acute inflammatory responses such as Crohn’s disease or ulcerative colitis.

Lactobacilli Reduce Chemokine IL-8 Production in Response to TNF-αandSalmonellaChallenge of Caco-2 Cells

“…These results show that both the structural components and the soluble cellular content of lactobacilli have an important function in anti-inflammatory effects. This observation is consistent with previous studies, which suggested that live probiotics are not necessarily required for anti-inflammatory effects [15, 33, 34]. However, Ma et al suggested that only live Lactobacillus reuteri inhibit TNF- α -induced IL-8 production in T84 and HT-29 cells and the inhibitory effect was not reproduced using CM, bacterial lysates, and heat-killed or gamma-irradiated lactobacilli [31].…”

In vitroadhesion and anti-inflammatory properties of nativeLactobacillus fermentumandLactobacillus delbrueckiispp.

“…This suggests that the stimulus required for induction of IL‐10 is different from that of IL‐6, TNF‐α and IL‐1β and that some secreted component of the bacteria may be responsible for its induction (Chon et al . ). Similar observation was made by Suzuki et al .…”

Oral administration of Lactobacillus plantarum K68 ameliorates DSS-induced ulcerative colitis in BALB/c mice via the anti-inflammatory and immunomodulatory activities

“…L. plantarum HY115 may inhibit the production of NO in LPSstimulated RAW 264.7 cells [22]. The ultrafiltrates of L. plantarum KFCC11389P metabolic products inhibited the production of TNF-α and IL-6 in LPS-induced RAW 264.7 cells [23]. In this study, the in vitro anti-inflammatory capacities of K68 isolated from fu-tsai were assessed by measuring the levels of the pro-inflammatory mediators NO, TNF-α, and PGE 2 .…”