Immunomodulatory Effects of Tigecycline in Balb/C Mice

Abstract

Tigecycline is a glycylcycline antibiotic approved by the FDA for the treatment of complicated infections. Despite its effectiveness, the FDA announced a warning of increasing mortality associated with its use. There is, however, no clear explanation for this side effect. Previous reports found a possible effect of tigecycline on leukocyte proliferation and proinflammatory cytokine release. We t herefore i nvestigated the effect of tigecycline on the immune components and response in Balb/c mice in vivo and in vitro. It was found that tigecycline enhanced lymphocyte proliferation and significantly increased cellular infiltration within the footpad, as based on DTH testing, but reduced the hemagglutination titer. In splenocyte cultures, tigecycline suppressed splenocyte proliferation with IC50 3-5 mmol L-1, significantly increased IL-2 secretion and reduced IL-17 secretion in a dose dependent mode. In conclusion, tigecycline is safe at therapeutic and sub-therapeutic doses, but it could still have an immunomodulatory effect at higher doses. Use of higher doses of tigecycline requires further investigation.

Pregabalin inhibits in vivo and in vitro cytokine secretion and attenuates spleen inflammation in Lipopolysaccharide/Concanavalin A -induced murine models of inflammation

“…Spleens were minced between the frosted edges of two microscopic slides and the cells were then pooled and centrifuged at 1200 rpm for 7 min at 4 °C. The red blood cells (RBC) within the pellets were then lysed at RT for 3 min using RBC lysis buffer as previously described 27,28 . Splenocytes were then suspended in 1 mL of RPMI-10% FBS and viable splenocyte count was determined using trypan blue dye exclusion method 29 .…”